[1]姚彬,葛腾,李越,等.中药干预对骨质疏松症大鼠骨密度及Wnt/β-catenin信号通路影响的Meta分析[J].中医正骨,2023,35(07):16-29.
　YAO Bin,GE Teng,LI Yue,et al.Effects of traditional Chinese drugs intervention on bone mineral density and Wnt/β-catenin signaling pathway in osteoporosis rats:a meta analysis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2023,35(07):16-29.
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中药干预对骨质疏松症大鼠骨密度及Wnt/β-catenin信号通路影响的Meta分析()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第35卷
期数:
2023年07期
页码:
16-29
栏目:
文献研究
出版日期:
2023-07-20

文章信息/Info

Title:
Effects of traditional Chinese drugs intervention on bone mineral density and Wnt/β-catenin signaling pathway in osteoporosis rats:a meta analysis
作者:
姚彬1; 葛腾1; 李越1; 康武林2; 杨锋2; 袁普卫2
(1.陕西中医药大学第一临床医学院,陕西 咸阳 712046; 2.陕西中医药大学附属医院,陕西 咸阳 712046)
Author(s):
YAO Bin1; GE Teng1; LI Yue1; KANG Wulin2; YANG Feng2; YUAN Puwei2
1.The First Clinical Medical College of Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi,China 2.The Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi,China
关键词:
骨质疏松;  模型; 动物;  大鼠;  中药;  Wnt信号通路;  骨密度;  专题Meta分析
Keywords:
osteoporosis;  models; animal;  rats;  traditional Chinese drugs;  Wnt signaling pathway;  bone density;  meta-analysis as topic
摘要:
目的:系统评价中药干预对骨质疏松症(osteoporosis,OP)大鼠骨密度及Wnt/β-联蛋白(β-catenin)信号通路的影响。方法:应用计算机检索中国知网、维普网、万方数据库、中国生物医学文献服务系统、PubMed、Embase和Cochrane Library中关于中药干预大鼠OP的文献,检索时限均为建库至2023年2月。实验组的干预措施为中药(包括中药复方、单味药、中药有效成分)灌胃,对照组的干预措施为生理盐水或蒸馏水灌胃。依据文献检索及筛选方案筛选出符合要求的文献后,由2名研究人员分别独立进行数据提取和质量评价。采用Stata13.0软件进行Meta分析。结果:共检索到210篇文献,最终纳入30篇文献,共涉及1105只大鼠,其中实验组750只,对照组355只。Meta分析结果显示,实验组大鼠股骨骨密度、腰椎骨密度及骨组织Wnt、β-catenin、Runt相关转录因子2、低密度脂蛋白受体相关蛋白5的mRNA和蛋白表达水平均高于对照组[SMD=1.75,95%CI(1.54,1.95),P=0.000; SMD=1.35,95%CI(1.07,1.64),P=0.000; SMD=3.82,95%CI(2.31,5.32),P=0.000; SMD=2.36,95%CI(2.00,2.72),P=0.000; SMD=3.07,95%CI(2.55,3.60),P=0.000; SMD=1.77,95%CI(1.40,2.14),P=0.000; SMD=5.11,95%CI(3.99,6.22),P=0.000; SMD=5.42,95%CI(4.04,6.81),P=0.000; SMD=1.74,95%CI(1.06,2.42),P=0.000; SMD=2.44,95%CI(1.47,3.41),P=0.000]。结论:中药干预能够提高OP大鼠的骨密度,其作用机制可能与其能够上调Wnt/β-catenin信号通路相关蛋白的表达有关。
Abstract:
Objective:To systematically review the effects of traditional Chinese drugs(TCDs)intervention on bone mineral density(BMD)and Wnt/β-catenin signaling pathway in osteoporosis(OP)rats.Methods:All the articles about intervention on OP rats with TCDs included from database establishing to February 2023 were retrieved from China National Knowledge Internet,Vip Database,Wanfang Database,Chinese Biomedical Literature Service System,PubMed,Embase and Cochrane Library through computer.The rats in experiment group were intervened by intragastric administration with TCDs including compounds(TCD),simplex drug and Chinese medicine effective constituents,while the ones in control group with normal saline or distilled water.The articles were screened according to the retrieval and screening scheme.The information was extracted and the methodological quality of researches in the articles was evaluated independently by two researchers,and if any disagreement was found between them,discussion was conducted or another researcher was invited for making a final decision,and then a Meta-analysis was conducted by using Stata13.0 software.Results:Two hundred and ten articles were searched out.After screening,30 articles(1105 rats)were included in the final analysis,750 rats in experiment group and 355 ones in control group.The results of Meta-analysis revealed that the BMDs of femur and lumbar vertebrae as well as the mRNA and protein expression levels of Wnt,β-catenin,Runt-related transcription factor 2(RUNX2)and low-density lipoprotein receptor-related protein 5(LRP5)in bone tissues were higher in experiment group compared to control group(SMD=1.75,95%CI(1.54,1.95),P=0.000; SMD=1.35,95%CI(1.07,1.64),P=0.000; SMD=3.82,95%CI(2.31,5.32),P=0.000; SMD=2.36,95%CI(2.00,2.72),P=0.000; SMD=3.07,95%CI(2.55,3.60),P=0.000; SMD=1.77,95%CI(1.40,2.14),P=0.000; SMD=5.11,95%CI(3.99,6.22),P=0.000; SMD=5.42,95%CI(4.04,6.81),P=0.000; SMD=1.74,95%CI(1.06,2.42),P=0.000; SMD=2.44,95%CI(1.47,3.41),P=0.000).Conclusion:TCDs intervention can improve the BMD of OP rats,and its mechanism of action may be that it can up-regulate the expression of Wnt/β-catenin signaling pathway-related proteins in OP rats.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81973889); 中医药“双链融合”中青年科研创新团队项目(2022-SLRH-LJ-001); 陕西省中医药重点研究室建设项目(陕中医药发〔2018〕32号) 通讯作者:袁普卫 E-mail:spine_surgeon@163.com
更新日期/Last Update: 1900-01-01