[1]李学朋,朱立国.骨疏康胶囊对去卵巢大鼠骨小梁的影响[J].中医正骨,2015,27(12):12-15.
　LI Xuepeng,ZHU Liguo.Effect of Gushukang Jiaonang(骨疏康胶囊)on bone trabecula in the ovariectomized rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(12):12-15.
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骨疏康胶囊对去卵巢大鼠骨小梁的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期数:
2015年12期
页码:
12-15
栏目:
基础研究
出版日期:
2015-12-30

文章信息/Info

Title:
Effect of Gushukang Jiaonang(骨疏康胶囊)on bone trabecula in the ovariectomized rats
作者:
李学朋; 朱立国
中国中医科学院望京医院,北京 100102
Author(s):
LI Xuepeng; ZHU Liguo
Wangjing Hospital of China Academy Of Chinese Medical Sciences,Beijing 100102,China
关键词:
骨质疏松;  骨疏康胶囊;  仙灵骨葆胶囊;  骨小梁;  形态计量法;  大鼠; Sprague-Dawley;  动物实验
Keywords:
osteoporosis;  Gushukang Jiaonang;  Xinling Gubao Jiaonang;  bone trabecula;  morphometry;  rats; Sprague-Dawley;  animal experimentation
摘要:
目的:观察骨疏康胶囊对去卵巢大鼠骨小梁的影响。方法:将120只6月龄雌性SD大鼠随机分为空白组、模型组、骨疏康组和仙灵骨葆组,每组30只。除空白组外,其余3组大鼠均切除双侧卵巢进行骨质疏松造模。造模手术3 d后,骨疏康组、仙灵骨葆组分别以骨疏康胶囊(4.32 g·kg-1·d-1)、仙灵骨葆胶囊(3 g·kg-1·d-1)与蒸馏水配成的药液灌胃,空白组和模型组均以等量蒸馏水灌胃,共持续30 d。药物干预结束后处死大鼠,取双侧股骨制成HE染色切片,测定并比较各组大鼠的股骨骨小梁面积、骨小梁厚度、骨小梁间距及骨小梁面积百分数。结果:4组大鼠股骨骨小梁厚度、骨小梁间距、骨小梁面积及骨小梁面积百分数比较,组间差异均有统计学意义[(118.74±8.93)μm,(52.00±6.49)μm,(107.00±9.02)μm,(85.00±4.38)μm,F=5.382,P=0.038;(258.37±78.48)μm,(564.19±135.83)μm,(301.37±89.54)μm,(426.48±98.32)μm,F=7.482,P=0.006;(648 092.35±11 353.46)μm2,(254 872.04±7 429.42)μm2,(589 435.58±10 374.58)μm2,(465 683.69±14 294.49)μm2,F=13.272,P=0.004;(0.49±0.02)%,(0.17±0.01)%,(0.41±0.03)%,(0.29±0.02)%,F=2.143,P=0.019]。模型组的骨小梁厚度、骨小梁面积及骨小梁面积百分数均小于空白组(P=0.002; P=0.001; P=0.014),骨小梁间距大于空白组(P=0.014); 骨疏康组和仙灵骨葆组的骨小梁厚度、骨小梁面积、骨小梁面积百分数均大于模型组(P=0.025; P=0.017; P=0.019; P=0.042; P=0.015; P=0.022),骨小梁间距均小于模型组(P=0.019; P=0.019); 骨疏康组的骨小梁厚度、骨小梁面积、骨小梁面积百分数均大于仙灵骨葆组(P=0.003; P=0.001; P=0.017),骨小梁间距小于仙灵骨葆组(P=0.016)。结论:骨疏康胶囊能明显增大去卵巢大鼠骨组织的骨小梁厚度、骨小梁面积及骨小梁面积百分数,减小骨小梁间距,其效果优于仙灵骨葆胶囊。
Abstract:
Objective:To observe the effect of Gushukang Jiaonang(骨疏康胶囊,GSKJN)on bone trabecula in the ovariectomized rats.Methods:One hundred and twenty 6-month-old female SD rats were randomly divided into blank group,model group,GSKJN group and Xianling Gubao Jiaonang(仙灵骨葆胶囊,XLGBJN)group,30 cases in each group.The rats in model group,GSKJN group and XLGBJN group were administrated with ovariectomy to build models of osteoporosis.Three days after modeling operation,the rats in GSKJN group and XLGBJN group were intragastric administrated with mixed solution of distilled water combined with GSKJN(4.32g/kg/day)and XLGBJN(3g/kg/day)respectively,while the rats in blank group and model group were intragastric administrated with equivalent distilled water for consecutive 30 days.After drug intervention,the rabbits were executed and their bilateral femurs were fetched out and sectioned for HE staining.Then the femoral trabecular area,trabecular thickness,trabecular interval and percentage of trabecular area were measured and compared between the 4 groups.Results:There were statistical differences in femoral trabecular thickness,trabecular interval,trabecular area and percentage of trabecular area between the 4 groups(118.74+/-8.93,52.00+/-6.49,107.00+/-9.02,85.00+/-4.38 μm,F=5.382,P=0.038; 258.37+/-78.48,564.19+/-135.83,301.37+/-89.54,426.48+/-98.32 μm,F=7.482,P=0.006; 648092.35+/-11353.46,254872.04+/-7429.42,589435.58+/-10374.58,465683.69+/-14294.49 μm(2),F=13.272,P=0.004; 0.49+/-0.02,0.17+/-0.01,0.41+/-0.03,0.29+/-0.02%,F=2.143,P=0.019).The trabecular thickness,trabecular area and percentage of trabecular area were less in model group compared with blank group(P=0.002; P=0.001; P=0.014),and the trabecular interval was greater in model group compared with blank group(P=0.014).The trabecular thickness,trabecular area and percentage of trabecular area were greater and the trabecular interval was less in GSKJN group and XLGBJN group compared with model group(P=0.025; P=0.017; P=0.019; P=0.042; P=0.015; P=0.022; P=0.019; P=0.019).The trabecular thickness,trabecular area and percentage of trabecular area were greater and the trabecular interval was less in GSKJN group compared with XLGBJN group(P=0.003; P=0.001; P=0.017; P=0.016).Conclusion:Oral application of GSKJN can obviously increase the trabecular thickness,trabecular area and percentage of trabecular area and decrease the trabecular interval in the ovariectomized rats,moreover,its therapeutic effect is better than that of XLGBJN.

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备注/Memo

备注/Memo:
2015-08-16收稿 2015-09-01修回
通讯作者:朱立国 E-mail:13583818129@163.com
更新日期/Last Update: 2015-12-30