[1]史晓林,李春雯,张志强.弱阳离子磁珠分离技术和基质辅助激光解吸电离飞行时间质谱技术在原发性Ⅰ型骨质疏松症血清标志蛋白筛选中的应用[J].中医正骨,2014,26(03):5-8.
　Shi Xiaolin*,Li Chunwen,Zhang Zhiqiang..Application of magnetic beads based weak cation exchange separation technology and matrix-assisted laser desorption-ionization time of flight mass spectrometry technology in screening serum protein markers of primary type-Ⅰ osteoporosis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(03):5-8.
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弱阳离子磁珠分离技术和基质辅助激光解吸电离飞行时间 质谱技术在原发性Ⅰ型骨质疏松症血清标志蛋白筛选中 的应用()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年03期
页码:
5-8
栏目:
基础研究
出版日期:
2014-03-30

文章信息/Info

Title:
Application of magnetic beads based weak cation exchange separation technology and matrix-assisted laser desorption-ionization time of flight mass spectrometry technology in screening serum protein markers of primary type-Ⅰ osteoporosis
作者:
史晓林1; 李春雯2; 张志强2
1.浙江中医药大学附属第二医院,浙江 杭州 310005;
2.浙江中医药大学,浙江 杭州 310053
Author(s):
Shi Xiaolin*; Li Chunwen; Zhang Zhiqiang.
*The Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou 310005,Zhejiang,China
关键词:
骨质疏松; 绝经后 弱阳离子磁珠 光谱法; 质量; 基质辅助激光解吸电离 蛋白质组学
Keywords:
Osteoporosis; Postmenopausal;  Magnetic beads based weak cation exchange;  Spectrometry; mass; matrix-assisted laser desorption-ionization;  Proteomics
摘要:
目的:探讨弱阳离子磁珠分离技术和基质辅助激光解吸电离飞行时间质谱技术在原发性Ⅰ型骨质疏松症血清标志蛋白筛选中的应用价值,寻找对原发性Ⅰ型骨质疏松症进行筛查和早期诊断的新方法。方法:应用弱阳离子磁珠分离技术和基质辅助激光解吸电离飞行时间质谱技术对16例绝经后骨质疏松症患者及9例绝经后健康妇女的血清蛋白进行筛选、分析,将经支持向量机模型预测的约登指数最高的差异蛋白峰作为候选标志物建立诊断模型,并对模型的判别效果进行验证。结果:2组血清蛋白标本共初选出123个递加质谱蛋白质峰。筛选出强度差异具有统计学意义的蛋白质峰16组,约登指数最高的4组差异蛋白质峰的质荷比分别为8 909.047、8 690.658、13 745.48、15 114.52。以这4个标志物作为候选标志物建立诊断模型,模型特异性、敏感性均为100%。2组标本在支持向量机结果散点图上可清晰区分。结论:采用弱阳离子磁珠分离技术和基质辅助激光解吸电离飞行时间质谱技术可筛选出原发性Ⅰ型骨质疏松症血清标志蛋白、建立诊断模型,为骨质疏松症的筛查和诊断提供了一种全新的血清学方法。
Abstract:
Objective:To evaluate the value of magnetic beads based weak cation exchange separation technology and matrix-assisted laser desorption-ionization time of flight mass spectrometry(MALDI-TOF-MS)technology in screening serum protein markers of primary type-Ⅰ osteoporosis,so as to find new methods for screening and early diagnosis of primary type-Ⅰ osteoporosis.Methods:The serum protein of 16 patients with postmenopausal osteoporosis and 9 postmenopausal healthy women were screened and analyzed using magnetic beads based weak cation exchange separation technology and MALDI-TOF-MS technology,and the differential protein peaks with the highest Youden index that predicted by support vector machine(SVM)model were chosen as candidate markers to build the diagnostic model,and then the distinguish effectiveness of the model was checked.Results:Totally 123 progressive protein peaks were originally selected from the protein peaks of 2 groups of serum protein specimens in the mass spectrogram.There was statistical differences in the intensity between 16 groups of protein peaks,and the mass charge ratio of 4 groups of differential protein peaks with the highest Youden index were 8 909.047,8 690.658,13 745.48 and 15 114.52 respectively.Then the 4 markers were used as candidate markers to build diagnostic model,and the specificity and sensitivity of the model were both 100%.The specimen in the 2 groups could be clearly distinguished by using scatterplot of SVM results.Conclusion:By using magnetic beads based weak cation exchange separation technology and MALDI-TOF-MS technology,serum protein markers of primary type-Ⅰ osteoporosis can be selected to build the diagnostic model,which provide a brand-new serological method for screening and diagnosis of osteoporosis.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81373878); 2012年浙江省自然科学基金项目(LY12H29007),2013年浙江省自然科学基金项目(LY13H290009)
通讯作者:李春雯 E-mail:chunwen2007@163.com
更新日期/Last Update: 2014-03-20