[1]翟晨骏,刘磊,周鸣,等.围手术期外周静脉血中长链非编码RNA PAX8-AS1与微RNA-1252-5p表达水平对胫骨骨折内固定术后骨折愈合的影响[J].中医正骨,2023,35(08):8-13.
　ZHAI Chenjun,LIU Lei,ZHOU Ming,et al.Effects of expression levels of long non-coding RNA PAX8-AS1 and microRNA-1252-5p in perioperative peripheral venous blood on fracture healing after internal fixation for treatment of tibial fractures[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2023,35(08):8-13.
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围手术期外周静脉血中长链非编码RNA PAX8-AS1与微RNA-1252-5p表达水平对胫骨骨折内固定术后骨折愈合的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第35卷
期数:
2023年08期
页码:
8-13
栏目:
临床研究
出版日期:
2023-08-20

文章信息/Info

Title:
Effects of expression levels of long non-coding RNA PAX8-AS1 and microRNA-1252-5p in perioperative peripheral venous blood on fracture healing after internal fixation for treatment of tibial fractures
作者:
翟晨骏; 刘磊; 周鸣; 贺健; 陈俊
宜兴市人民医院,江苏 宜兴 214200
Author(s):
ZHAI Chenjun; LIU Lei; ZHOU Ming; HE Jian; CHEN Jun
Yixing People's Hospital,Yixing 214200,Jiangsu,China
关键词:
胫骨骨折;  骨折愈合;  RNA; 长链非编码;  微RNAs;  预测;  生物标志物
Keywords:
tibial fractures;  fracture healing;  RNA; long noncoding;  microRNAs;  forecasting;  biomarkers
摘要:
目的:探讨围手术期外周静脉血中长链非编码RNA(long non-coding RNA,lncRNA)PAX8-AS1及微RNA(microRNA,miRNA)-1252-5p表达水平对胫骨骨折内固定术后骨折愈合的影响。方法:以2018年1月至2020年12月在宜兴市人民医院接受同一组医生完成的钢板内固定或髓内钉内固定手术治疗的胫骨骨折患者为研究对象。收集患者的性别、年龄、骨折类型、损伤类型、手术方式、吸烟史、饮酒史、高血压史、糖尿病史等一般资料,随访观察骨折愈合情况。采用荧光定量PCR检测术前及术后1周、2周、4周、8周时患者外周静脉血中lncRNA PAX8-AS1、miRNA-1252-5p的表达水平。根据术后4个月时的骨折愈合情况,将纳入的病例分为正常愈合组及延迟愈合组。比较2组患者的一般资料及手术前后外周静脉血中lncRNA PAX8-AS1、miRNA-1252-5p表达水平,分析影响骨折愈合的因素,评价外周静脉血中lncRNA PAX8-AS1、miRNA-1252-5p表达水平对骨折延迟愈合的预测价值。结果:①一般结果。共纳入250例患者,正常愈合组210例、延迟愈合组40例。延迟愈合组合并糖尿病比例、开放性骨折比例、高能量损伤比例均高于正常愈合组(χ2=28.245,P=0.000; χ2=24.881,P=0.000; χ2=34.469,P=0.000)。②外周静脉血中lncRNA PAX8-AS1、miRNA-1252-5p表达水平检测结果。术后2周和术后4周时,延迟愈合组外周静脉血中lncRNA PAX8-AS1表达水平均高于正常愈合组(t=4.742,P=0.000; t=2.753,P=0.006),miRNA-1252-5p表达水平均低于正常愈合组(t=4.079,P=0.000; t=2.538,P=0.012)。③胫骨骨折内固定术后骨折愈合影响因素分析结果。广义估计方程分析结果显示,开放性骨折、高能量损伤及术后2周时外周静脉血中lncRNA PAX8-AS1表达水平升高和miRNA-1252-5p表达水平降低是胫骨骨折内固定术后骨折愈合的影响因素(β=29.382,Wald χ2=8.589,P=0.000; β=22.771,Wald χ2=9.766,P=0.000; β=57.418,Wald χ2=13.735,P=0.000; β=-81.524,Wald χ2=2.392,P=0.000)。④外周静脉血中lncRNA PAX8-AS1、miRNA-1252-5p表达水平对胫骨骨折内固定术后骨折延迟愈合的预测价值评价结果。受试者操作特征(receiver operating characteristic,ROC)曲线分析结果显示,术后2周时外周静脉血中lncRNA PAX8-AS1、miRNA-1252-5p表达水平预测胫骨骨折内固定术后骨折延迟愈合的ROC曲线下面积分别为0.728(P=0.000)和0.679(P=0.000),灵敏度分别为74.76%和53.33%,特异度分别为62.50%和75.00%。结论:胫骨骨折内固定术后2周时,外周静脉血中lncRNA PAX8-AS1表达水平升高和miRNANA-1252-5p表达水平降低,对预测胫骨骨折内固定术后骨折延迟愈合具有一定的价值。
Abstract:
Objective:To explore the effects of expression levels of long non-coding RNA(lncRNA)PAX8-AS1 and microRNA(miRNA)-1252-5p in perioperative peripheral venous blood(PVB)on fracture healing after internal fixation for treatment of tibial fractures.Methods:The patients who underwent internal fixation with steel plate or intramedullary nail for treatment of tibial fractures by the same surgeons in Yixing People's Hospital from January 2018 to December 2020 were selected as the subjects,and their information including gender,age,fracture type,injury type,surgery method,smoking history,drinking history,hypertension history and diabetes mellitus history was collected.The patients were followed up and the fracture healing was observed.The expression levels of lncRNA PAX8-AS1 and miRNA-1252-5p in PVB were detected by using fluorescent quantitative PCR method before the surgery and at postsurgical week 1,2,4 and 8.According to the fracture healing at postsurgical month 4,the included patients were divided into normal healing group and delayed healing group.The general data of patients and the presurgical and postsurgical expression levels of lncRNA PAX8-AS1 and miRNA-1252-5p in PVB were compared between the 2 groups.Moreover,the factors influencing fracture healing were analyzed,and the value of the expression levels of lncRNA PAX8-AS1 and miRNA-1252-5p in PVB in predicting delayed fracture healing was evaluated.Results:①Two hundred and fifty patients were enrolled in the study,210 cases in normal healing group and 40 cases in delayed healing group.The proportions of combined diabetes mellitus,open fracture and high-energy injury were higher in delayed healing group compared to normal healing group(χ2=28.245,P=0.000; χ2=24.881,P=0.000; χ2=34.469,P=0.000).②The expression level of lncRNA PAX8-AS1 in PVB was higher and the expression level of miRNA-1252-5p in PVB was lower in delayed healing group compared to normal healing group at postsurgical week 2 and 4(t=4.742,P=0.000; t=2.753,P=0.006; t=4.079,P=0.000; t=2.538,P=0.012).③The factors influencing fracture healing after internal fixation for treatment of tibial fractures were analyzed by using generalized estimating equation(GEE),and the results showed that open fracture,high-energy injury as well as increased expression level of lncRNA PAX8-AS1 and decreased expression level of miRNA-1252-5p in PVB at postsurgical week 2 were the factors influencing fracture healing after internal fixation for tibial fractures(β=29.382,Wald χ2=8.589,P=0.000; β=22.771,Wald χ2=9.766,P=0.000; β=57.418,Wald χ2=13.735,P=0.000; β=-81.524,Wald χ2=2.392,P=0.000).④The applied value of expression levels of lncRNA PAX8-AS1 and miRNA-1252-5p in PVB in predicting delayed fracture healing after internal fixation for treatment of tibial fractures was evaluated and analyzed by using receiver operating characteristic(ROC)curve,and the results showed that,at postsurgical week 2,the areas under the ROC curves were 0.728(P=0.000)and 0.679(P=0.000),the sensitivities were 74.76% and 53.33%,and the specificities were 62.50% and 75.00% respectively.Conclusion:At postsurgical week 2,the increased expression level of lncRNA PAX8-AS1 and the decreased expression level of miRNA-1252-5p in PVB have a certain application value in prediction of delayed fracture healing after internal fixation for treatment of tibial fractures.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(82202716) 通讯作者:陈俊 E-mail:staff928@yxph.com
更新日期/Last Update: 1900-01-01