[1]楼鹏强,邓小磊,魏波,等.2种冷冻时长进行液氮冷冻法犬股骨头坏死造模的效果比较[J].中医正骨,2022,34(03):3-7.
　LOU Pengqiang,DENG Xiaolei,WEI Bo,et al.A comparative analysis of canine femur head necrosis models induced by liquid nitrogen freezing with two freezing durations[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2022,34(03):3-7.
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2种冷冻时长进行液氮冷冻法犬股骨头坏死造模的效果比较()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第34卷
期数:
2022年03期
页码:
3-7
栏目:
基础研究
出版日期:
2022-03-20

文章信息/Info

Title:
A comparative analysis of canine femur head necrosis models induced by liquid nitrogen freezing with two freezing durations
作者:
楼鹏强; 邓小磊; 魏波; 汤朔; 侯德才
(辽宁中医药大学附属医院,辽宁 沈阳 110032)
Author(s):
LOU Pengqiang; DENG Xiaolei; WEI Bo; TANG Shuo; HOU Decai
The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China
关键词:
股骨头坏死;  模型; 动物;  犬;  液氮
Keywords:
femur head necrosis;  models; animal;  canis familiaris;  liquid nitrogen
摘要:
目的:比较2种冷冻时长进行液氮冷冻法犬股骨头坏死造模的效果。方法:将12只SPF级健康成年雄性比格犬随机分为2组,每组6只。均采用液氮冷冻法进行股骨头坏死造模,液氮冷冻时长分别为8 min(冷冻8 min组)和4 min(冷冻4 min组)。造模手术后观察并记录动物的一般情况。造模后9周,进行X线和MRI检查,并以MRI影像作为判断股骨头坏死的标准。影像学检查结束后,处死动物,取出造模侧股骨头,进行大体观察和病理学检查(HE染色)。结果:①一般情况。至最终处死前,2组均无动物死亡。造模手术后2 d内,所有动物均开始出现饮食减少、精神萎靡、活动减少等表现。冷冻4 min组动物的上述表现均在1周内改善; 冷冻8 min组动物的上述表现约在2周后逐步恢复正常,切口愈合缓慢,而且均出现了跛行现象。冷冻8 min组的1只动物在造模手术后第3天出现术区皮肤红肿,造模手术后1周时出现对应部位皮肤组织坏死及化脓,造模手术后4周时愈合。②影像学检查结果。X线片显示,冷冻8 min组动物造模侧股骨头均出现了不同程度的变化,如骨质硬化、关节间隙变窄,但变化不明显,且部分动物的股骨头出现了囊性变; 冷冻4 min组仅2只动物的股骨头出现了轻微的骨质硬化。MRI显示,冷冻8 min组所有动物T2WI上均可见股骨头骨髓水肿和关节腔积液表现,T1WI上均可见线样低信号,均达到股骨头坏死标准; 冷冻4 min组4只动物T2WI上可见股骨头骨髓水肿和关节腔积液表现,其余2只动物的T2WI上仅可见关节腔积液表现,T1WI上均无明显改变。③大体观察结果。冷冻8 min组股骨头扁平、表面较粗糙,部分股骨头出现软骨裂纹,部分股骨头软骨磨损严重; 冷冻4 min组股骨头形态无明显改变,表面较光滑,仅部分动物的股骨头出现了软骨裂纹或轻微软骨磨损。④病理学检查结果。冷冻8 min组股骨头组织骨陷窝内大部分骨细胞核消失,同时可见骨小梁断裂; 冷冻4 min组股骨头组织大部分骨陷窝内可见骨细胞核,骨小梁完整。冷冻8 min组股骨头组织空骨陷窝率高于冷冻4 min组[(20.21±2.6)%,(13.51±3.1)%,t=4.055,P=0.002]。结论:以液氮冷冻法进行犬股骨头坏死造模时,液氮冷冻8 min的造模成功率高于液氮冷冻4 min。
Abstract:
Objective:To compare the effects of canine femur head necrosis(FHN)models induced by liquid nitrogen freezing(LNF)with two freezing durations.Methods:Twelve SPF-grade healthy adult male beagle canines were selected and randomly assigned to 2 groups,6 ones in each group,and they were subjected to LNF for 8 minutes(8-min freezing group)and 4 minutes(4-min freezing group)respectively for inducing FHN.The general conditions of the model canines were then observed and recorded after the modeling surgery.At 9 weeks after the modeling,all model canines underwent X-ray examination and magnetic resonance imaging(MRI)examination and the MRI images were used as the standard for confirming whether the FHN models were built successfully.After the end of imaging examination,the model canines were sacrificed and their femur heads were harvested from the modeling side for gross observation and pathological examination(HE staining).Results:①No model canine died in the 2 groups before the final execution.Within 2 days after the modeling surgery,all model canines began to exhibit such symptoms as decreased food intake,mental fatigue and reduced movement,which was improved within 1 week in 4-min freezing group,and gradually returned to normal about 2 weeks later in 8-min freezing group with the symptoms of slow wound healing and claudication.One model canine in 8-min freezing group exhibited the symptoms as skin redness and swelling on the 3rd day after the modeling surgery,skin tissue necrosis and pyosis at the corresponding site at 1 week after the modeling surgery,and healed at 4 weeks after the modeling surgery.②The results of X-ray examination showed that the unremarkable changes in different degrees,manifesting as osteosclerosis and joint space narrowing,were observed in femur heads at the modeling side of model canine in 8-min freezing group,and cystic changes in the femur heads were further observed in some model canines; while the slight osteosclerosis in femur heads was observed in only 2 model canines in 4-min freezing group.As revealed by MRI images,the bone marrow edema(BME)of femur heads and articular cavity effusion on T2WI and cord-like low signals on T1WI were observed in all model canines of 8-min freezing group,which met the diagnosis criteria of FHN; while,for model canines in 4-min freezing group,the BME of femur heads and articular cavity effusion on T2WI were observed in only 4 ones,and articular cavity effusion alone on T2WI were observed in the other 2 ones,and no significant changes on T1WI was found in all model canines.③The flattened femur heads with rough surfaces were observed in model canines of 8-min freezing group,furthermore,some ones presented with cartilage cracks and the other ones with severely worn cartilage; while,for model canines in 4-min freezing group,the femur heads were comparatively smooth without obvious changes in the morphology,just cartilage cracks or slight cartilage wear were observed in only some ones.④Most of the osteocyte nuclei disappeared within the bone lacunae of femoral head tissues in model canines of 8-min freezing group,and the bone trabeculae fractures was observed; while the osteocyte nuclei was observed within most of the bone lacunae of femoral head tissues in model canines of 4-min freezing group,and the bone trabeculae were intact.The percentage of empty bone lacunae in femoral head tissues was higher in 8-min freezing group compared to 4-min freezing group(20.21±2.6 vs 13.51±3.1%,t=4.055,P=0.002).Conclusion:The success rate is higher in canine FHN model induced by LNF for 8 minutes compared to the ones for 4 minutes.

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(收稿日期:2021-11-14 本文编辑:李晓乐)

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备注/Memo

备注/Memo:
基金项目:辽宁省科学技术计划项目(2017225043) 通讯作者:侯德才 E-mail:lnzyhdc@163.com
更新日期/Last Update: 1900-01-01