[1]周勇,任菲菲,丰凡翔,等.血管内皮生长因子和骨形态发生蛋白2 在非创伤性股骨头坏死不同区域的表达[J].中医正骨,2015,27(08):7-10.
 ZHOU Yong,REN Feifei,FENG Fanxiang,et al.Expressions of vascular endothelial growth factor and bone morphogenetic protein 2 in different zones of femoral head with non-traumatic osteonecrosis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(08):7-10.
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血管内皮生长因子和骨形态发生蛋白2 在非创伤性股骨头坏死不同区域的表达()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期数:
2015年08期
页码:
7-10
栏目:
基础研究
出版日期:
2015-08-30

文章信息/Info

Title:
Expressions of vascular endothelial growth factor and bone morphogenetic protein 2 in different zones of femoral head with non-traumatic osteonecrosis
作者:
周勇1任菲菲1丰凡翔2徐祖健2
1.四川省双流县中医医院,四川 双流 610200;
2.泸州医学院附属中医医院,四川 泸州 646000
Author(s):
ZHOU Yong1REN Feifei1FENG Fanxiang2XU Zujian2
1.Shuangliu Hospital of Traditional Chinese Medicine,Shuangliu 610200,Sichuan,China
2.The affiliated hospital of traditional Chinese medicine of Luzhou medical college,Luzhou 646000,Sichuan,China
关键词:
股骨头坏死 血管内皮生长因子类 骨形态发生蛋白质2 免疫组织化学
Keywords:
femur head necrosis vascular endothelial growth factors bone morphogenetic protein 2 immunohistochemistry
摘要:
目的:探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)和骨形态发生蛋白2(bone morphogenetic protein 2,BMP-2)在非创伤性股骨头坏死不同区域的表达情况。方法:将从单侧人工髋关节置换术中取得的40个非创伤性坏死股骨头沿冠状面自正中用摆锯剖开,分别在坏死区、交界区和正常区各凿取1块约1 cm×1 cm×0.4 cm的骨块,制成石蜡切片,采用免疫组织化学法测定不同区域VEGF和BMP-2的表达情况。结果:①VEGF表达结果。3个区域VEGF阳性面积百分比比较,差异有统计学意义[(6.25±2.61)%,(19.30±4.56)%,(11.63±4.12)%; χ2=81.100,P=0.000]; 坏死区阳性面积百分比小于交界区和正常区(χ2=81.040,P=0.000; χ2=22.200,P=0.000),交界区大于正常区(χ2=18.410,P=0.000)。3个区域VEGF表达平均灰度比较,差异有统计学意义(86.30±6.84,31.85±4.89,73.93±5.78; F=939.984,P=0.000); 坏死区平均灰度大于交界区和正常区(P=0.000; P=0.000),交界区小于正常区(P=0.000)。②BMP-2的表达结果。3个区域的BMP-2阳性面积百分比比较,差异有统计学意义[(5.20±2.46)%,(17.43±4.77)%,(10.03±4.32)%; χ2=76.450,P=0.000]; 坏死区阳性面积百分比小于交界区和正常区(χ2=76.320,P=0.000; χ2=21.950,P=0.000),交界区大于正常区(χ2=27.400,P=0.000)。3个区域BMP-2表达平均灰度比较,差异有统计学意义(88.65±6.61,41.25±5.00,76.80±5.33; F=751.985,P=0.000); 坏死区BMP-2表达平均灰度大于交界区和正常区(P=0.000; P=0.000),交界区小于正常区(P=0.000)。结论:VEGF和BMP-2在非创伤性股骨头坏死交界区表达强度高于坏死区和正常区,交界区可能是影响股骨头坏死预后的关键区域。
Abstract:
Objective:To explore the expressions of vascular endothelial growth factor(VEGF)and bone morphogenetic protein 2(BMP-2)in different zones of femoral head with non-traumatic osteonecrosis.Methods:Forty non-traumatic necrotic femoral head achieved via unilateral artificial hip replacement were splited along the median coronal plane by using swing saw,and a 1.0×1.0×0.4-cm specimen was fetched from the necrotic zone,junctional zone and normal zone respectively and was made into paraffin sections.Then the expressions of VEGF and BMP-2 were detected by using immunohistochemical method.Results:There was statistical difference in the positive area percentage of VEGF between the 3 zones(6.25+/-2.61,19.30+/-4.56,11.63+/-4.12%; χ2=81.100,P=0.000),and the necrotic zone was inferior to the junctional zone and normal zone(χ2=81.040,P=0.000; χ2=22.200,P=0.000)while the junctional zone surpassed the normal zone(χ2=18.410,P=0.000).There was statistical difference in the average gray value of VEGF expression between the 3 zones(86.30+/-6.84,31.85+/-4.89,73.93+/-5.78; F=939.984,P=0.000),and the necrotic zone surpassed the junctional zone and normal zone(P=0.000; P=0.000)while the junctional zone was inferior to the normal zone(P=0.000).There was statistical difference in the positive area percentage of BMP-2 between the 3 zones(5.20+/-2.46,17.43+/-4.77,10.03+/-4.32%; χ2=76.450,P=0.000),and the necrotic zone was inferior to the junctional zone and normal zone(χ2=76.320,P=0.000; χ2=21.950,P=0.000)while the junctional zone surpassed the normal zone(χ2=27.400,P=0.000).There was statistical difference in the average gray value of BMP-2 expression between the 3 zones(88.65+/-6.61,41.25+/-5.00,76.80+/-5.33; F=751.985,P=0.000),and the necrotic zone surpassed the junctional zone and normal zone(P=0.000; P=0.000)while the junctional zone was inferior to the normal zone.Conclusion:The expression intensity of VEGF and BMP-2 of the junctional zone of non-traumatic femoral head necrosis is higher than that of necrotic zone and normal zone,therefore the junctional zone may be the key zone which will influence the prognosis of femoral head necrosis.

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备注/Memo

备注/Memo:
2015-05-08收稿 2015-07-17修回
基金项目:四川省卫生厅科研项目(100238)
通讯作者:徐祖健 E-mail:xuzujian@21cn.com
更新日期/Last Update: 2015-08-30