[1]尤卓,熊辉,吴海金,等.蠲痹历节清方对急性痛风性关节炎大鼠的影响及其作用机制[J].中医正骨,2018,30(02):1-6,15.
　YOU Zhuo,XIONG Hui,WU Haijin,et al.Effect of Juanbi Lijie Qing Fang(蠲痹历节清方)on rats with acute gouty arthritis and its mechanism of action[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(02):1-6,15.
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蠲痹历节清方对急性痛风性关节炎大鼠的影响及其作用机制()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第30卷
期数:
2018年02期
页码:
1-6,15
栏目:
基础研究
出版日期:
2018-02-20

文章信息/Info

Title:
Effect of Juanbi Lijie Qing Fang(蠲痹历节清方)on rats with acute gouty arthritis and its mechanism of action
作者:
尤卓1; 熊辉1; 吴海金1; 郭玉星2; 周彪1; 齐新宇1; 向黎黎2
1.湖南中医药大学,湖南 长沙 410208; 2.湖南中医药大学第二附属医院,湖南 长沙 410005
Author(s):
YOU Zhuo1; XIONG Hui1; WU Haijin1; GUO Yuxing2; ZHOU Biao1; QI Xinyu1; XIANG Lili2
1.Hunan Chinese Medical University,Changsha 410208,Hunan,China 2.The Second Affiliated Hospital of Hunan Chinese Medical University,Changsha 410005,Hunan,China
关键词:
关节炎; 痛风性;  蠲痹历节清方;  尿酸;  肿瘤坏死因子α;  白细胞介素1β;  白细胞介素6;  大鼠;  动物实验
Keywords:
Keywords arthritis; gouty;  Juanbi Lijie Qing Fang;  uric acid;  tumor necrosis factor-alpha;  interleukin-1beta;  interleukin-6;  rats;  animal experimentation
摘要:
目的:探讨蠲痹历节清方对急性痛风性关节炎大鼠的影响及其作用机制。方法:将96只2月龄雄性清洁级健康SD大鼠随机分为正常对照组、模型组、蠲痹历节清方组、依托考昔组,每组24只。模型组、蠲痹历节清方组、依托考昔组大鼠以氧嗪酸钾溶液连续腹腔注射后,于大鼠右侧踝关节腔内注射尿酸钠溶液; 正常对照组大鼠腹腔及右侧踝关节腔分别注入等剂量生理盐水。关节腔内注射后即刻给予药物干预,正常对照组和模型组以10 mL·kg-1生理盐水灌胃,蠲痹历节清方组以22 g·kg-1剂量的蠲痹历节清方悬混液灌胃,依托考昔组以5.5 mg·kg-1剂量的依托考昔悬混液灌胃; 每日2次,连续灌胃2 d。分别于药物干预开始后4 h、12 h、24 h、48 h采用免疫组化法检测各组大鼠踝关节滑膜组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素
Abstract:
ABSTRACT Objective:To explore the effects of Juanbi Lijie Qing Fang(蠲痹历节清方,JBLJQF)on rats with acute gouty arthritis and its mechanism of action.Methods:Ninety-six 2-month-old healthy clean-grade male SD rats were randomly divided into normal control group,model group,JBLJQF group and etoricoxib group,24 cases in each group.The rats in model group,JBLJQF group and etoricoxib group were intraperitoneal injected continuously with oteracil potassium solutions,and then the sodium urate solutions were injected into their right ankles,while the rats in normal control group were administrated with intraperitoneal injection and intra-articular injection of the same dose of normal saline(NS)respectively.Drug interventions were performed on the rats immediately after intra-articular injection.The rats in normal control group,model group,JBLJQF group and etoricoxib group were intragastric administrated with NS(10 mL/kg),NS(10 mL/kg),JBLJQF suspension(22 g/kg)and etoricoxib suspension(5.5 mg/kg)respectively,twice a day for 2 consecutive days.The contents of tumor necrosis factor-α(TNF-α)protein,interleukin-1β(IL-1β)protein and interleukin-6(IL-6)protein in ankle synovial tissues were detected by using immunohistochemical method at 4,12,24 and 48 hours after the beginning of drug intervention respectively.The blood uric acid(UA)contents were detected by using full-

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81574005) 通讯作者:熊辉 E-mail:xh_hn@sina.com
更新日期/Last Update: 2018-07-02