[1]齐新宇,熊辉,周彪,等.蠲痹历节清方干预鸡急性痛风性关节炎模型的 实验研究[J].中医正骨,2015,27(03):5-11.
　QI Xinyu,XIONG Hui,ZHOU Biao,et al.Juanbilijieqing Fang(蠲痹历节清方)interfere with acute gouty arthritis chiken model[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):5-11.
点击复制

蠲痹历节清方干预鸡急性痛风性关节炎模型的 实验研究()
分享到：

《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期数:
2015年03期
页码:
5-11
栏目:
基础研究
出版日期:
2015-03-30

文章信息/Info

Title:
Juanbilijieqing Fang(蠲痹历节清方)interfere with acute gouty arthritis chiken model
作者:
齐新宇1; 熊辉2; 周彪1; 向黎黎1; 李腾龙1; 郭玉星1; 陆小龙2
1.湖南中医药大学,湖南 长沙 410208;
2.湖南中医药大学第二附属医院,湖南 长沙 410005
Author(s):
QI Xinyu1; XIONG Hui2; ZHOU Biao1; XIANG Lili1; LI Tenglong1; GUO Yuxing1; LU Xiaolong2
1.Hunan University of Traditional Chinese Medicine,Changsha 410208,Hunan,China
2.The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China
关键词:
关节炎; 痛风性;  蠲痹历节清方;  疾病模型; 动物;  尿酸;  黄嘌呤氧化酶;  动物实验
Keywords:
arthritis; gouty;  Juanbilijieqing Fang;  disease models; animal;  uric acid;  xanthine oxidase;  animal experimentation
摘要:
目的:观察蠲痹历节清方干预鸡急性痛风性关节炎模型的疗效,并探讨其可能的作用机制。方法:将160只30日龄雄性湘黄鸡随机分为对照组、模型组、蠲痹历节清方组及别嘌醇组,每组40只。对照组以正常饲料喂养; 其余3组以蛋白含量为50%的饲料喂养,连续喂养21 d制成急性痛风性关节炎模型。自造模结束后第1天开始,蠲痹历节清方组及别嘌醇组分别以蠲痹历节清方汤剂和别嘌醇溶液灌胃,其余2组均以生理盐水灌胃,每日2次,共21 d。分别于药物干预开始后1、7、14、21 d在每组随机选出10只鸡,分别观察其一般状态、踝关节周径、血尿酸含量和血清黄嘌呤氧化酶(xanthine oxidase,XOD)活性,随后处死动物,分离出左侧踝关节滑膜组织,光镜下观察其滑膜组织形态。结果:①一般状态。除对照组外,其余3组动物药物干预开始时精神萎顿,羽毛松乱、灰暗无光泽,饮食减退,双膝与双踝关节肿大,跛行,站立不稳,粪便中白色物质增多。至药物干预后21 d时,蠲痹历节清方组和别嘌醇组动物一般状态基本恢复正常; 模型组动物一般状态较开始时好转,但表现较蠲痹历节清方组和别嘌醇组差; 对照组动物除体质量增加外,其余表现与造模前一致。造模及实验过程中各组均无动物死亡。②踝关节周径。药物干预开始后各时点,4组动物踝关节周径比较,组间差异均有统计学意义(F=25.172,P=0.000; F=24.445,P=0.000; F=21.237,P=0.014; F=29.881,P=0.041)。药物干预开始后1、7、14 d时,模型组、蠲痹历节清方组及别嘌醇组踝关节周径均大于对照组(P=0.001,P=0.001,P=0.001; P=0.002,P=0.002,P=0.001; P=0.001,P=0.002,P=0.001); 药物干预开始后7、14 d时,蠲痹历节清方组和别嘌醇组踝关节周径均小于模型组(P=0.025,P=0.014; P=0.012,P=0.011); 药物干预开始后21 d时,对照组、蠲痹历节清方组及别嘌醇组踝关节周径均小于模型组(P=0.001,P=0.015,P=0.013); 其余各时点各组间两两比较,组间差异均无统计学意义。③血尿酸含量。药物干预开始后各时点,4组动物血尿酸含量比较,组间差异均有统计学意义(F=25.361,P=0.003; F=32.371,P=0.005; F=36.734,P=0.021; F=48.336,P=0.023)。药物干预开始后1、7、14 d时,模型组、蠲痹历节清方组及别嘌醇组血尿酸含量均大于对照组(P=0.000,P=0.001,P=0.001; P=0.001,P=0.001,P=0.002; P=0.001,P=0.000,P=0.001); 药物干预开始后7、14 d时,蠲痹历节清方组和别嘌醇组血尿酸含量均小于模型组(P=0.011,P=0.010; P=0.014,P=0.002); 药物干预开始后21 d时,对照组、蠲痹历节清方组及别嘌醇组血尿酸含量均小于模型组(P=0.001,P=0.012,P=0.011); 其余各时点各组间两两比较,组间差异均无统计学意义。④血清XOD活性。药物干预开始后各时点,4组动物血清XOD活性比较,组间差异均有统计学意义(F=45.721,P=0.001; F=50.634,P=0.002; F=49.448,P=0.013; F=63.124,P=0.027)。药物干预开始后1、7、14 d时,模型组、蠲痹历节清方组及别嘌醇组血清XOD活性均高于对照组(P=0.002,P=0.001,P=0.001; P=0.001,P=0.000,P=0.001; P=0.001,P=0.001,P=0.000); 药物干预开始后7、14 d时,蠲痹历节清方组和别嘌醇组血清XOD活性均低于模型组(P=0.001,P=0.013; P=0.002,P=0.015); 药物干预开始后21 d时,对照组、蠲痹历节清方组及别嘌醇组血清XOD活性均低于模型组(P=0.001,P=0.013,P=0.017); 其余各时点各组间两两比较,组间差异均无统计学意义。⑤滑膜组织形态。药物干预开始后各时点,4组动物滑膜中血管数量比较,组间差异均有统计学意义(F=24.772,P=0.032; F=33.176,P=0.021; F=32.672,P=0.003; F=44.351,P=0.000)。药物干预开始后1、7、14 、21 d时,模型组、蠲痹历节清方组及别嘌醇组滑膜中血管数量均多于对照组(P=0.002,P=0.001,P=0.001; P=0.001,P=0.001,P=0.002; P=0.001,P=0.000,P=0.001; P=0.001,P=0.003,P=0.002); 药物干预开始后7、14、21 d时,蠲痹历节清方组和别嘌醇组滑膜中血管数量均多于模型组(P=0.001,P=0.013; P=0.014,P=0.011; P=0.001,P=0.012); 其余各时点各组间两两比较,组间差异均无统计学意义。药物干预开始后各时点,4组动物滑膜中中性粒细胞数量比较,组间差异均有统计学意义(F=32.347,P=0.001; F=43.561,P=0.001; F=42.361,P=0.014; F=51.745,P=0.011)。药物干预开始后1、7、14 d时,模型组、蠲痹历节清方组及别嘌醇组滑膜中中性粒细胞数量均多于对照组(P=0.001,P=0.002,P=0.001; P=0.001,P=0.001,P=0.002; P=0.002,P=0.001,P= 0.001); 药物干预开始后7、14 d时,蠲痹历节清方组和别嘌醇组滑膜中中性粒细胞数量均少于模型组(P=0.017,P=0.014; P=0.012,P=0.014); 药物干预开始后21 d时,对照组、蠲痹历节清方组及别嘌醇组滑膜中中性粒细胞数量均少于模型组(P=0.001,P=0.013,P=0.017); 其余各时点各组间两两比较,组间差异均无统计学意义。结论:蠲痹历节清方可通过抑制XOD活性,有效降低血尿酸水平,缓解鸡急性痛风性关节炎模型临床症状,其疗效与别嘌醇相当。
Abstract:
Objective:Objective: To observe the effect of Juanbilijieqing Fang(蠲痹历节清方)on acute gouty arthritis chiken model and to explore the mechanism of action.Methods:One hundred and sixty 30-day-old male XIANGHUANG chickens were randomly divided into control group,model group,Juanbilijieqing group and allopurinol group,40 cases in each group.The chickens in control group were fed with normal feedstuff,while the chickens in other groups were fed for consecutive 21 days with feedstuff which protein content was 50% to made into acute gouty arthritis models.After the end of the modeling,the chickens in Juanbilijieqing group and allopurinol group were intragastric administrated with Juanbilijieqing decoction and allopurinol solution respectively,while chickens in the other 2 groups were intragastric administrated with normal saline,twice a day for 21 consecutive days.Ten chickens were randomly selected from each group at 1,7,14 and 21 days after the beginning of drug intervention respectively; and the general state,ankle circumference,blood uric acid levels and serum xanthine oxidase(XOD)activity were observed respectively.Then the chickens were executed and the left ankle synovium were separated for observing the synovial tissue morphology under the optical microscope.Results:The chickens except those in control group became lethargic and their feathers became disorderly loose and matte and their eating and drinking began to decrease.Both knees and ankles began to swell up,and limping and unstable standing were found.White matters increased in feces.The general state of chickens in Juanbilijieqing group and allopurinol group basically returned to normal at 21 days after the beginning of the drug intervention.The chickens in model group improved in the general state,while their performance were worse than that of chickens in Juanbilijieqing group and allopurinol group.No change except increased body mass were found in the chickens in control group.No chickens died in each group during the modeling and experimental process.There was statistical difference in ankle circumference between the 4 groups at different timepoints after the beginning of the drug intervention(F=25.172,P=0.000; F=24.445,P=0.000; F=21.237,P=0.014; F=29.881,P=0.041).The ankle circumference was greater in model group,Juanbilijieqing group and allopurinol group compared with control group at 1,7 and 14 days after the beginning of the drug intervention(P=0.001,P=0.001,P=0.001; P=0.002,P=0.002,P=0.001; P=0.001,P=0.002,P=0.001).The ankle circumference was less in Juanbilijieqing group and allopurinol group compared with model group at 7 and 14 days after the beginning of the drug intervention(P=0.025,P=0.014; P=0.012,P=0.011).The ankle circumference was less in control group,Juanbilijieqing group and allopurinol group compared with model group at 21 days after the beginning of the drug intervention(P=0.001,P=0.015,P=0.013).There was no statistical difference in the ankle circumference between the paired groups at other timepoints.There was statistical difference in blood uric acid levels between the 4 groups at different timepoints after the beginning of the drug intervention(F=25.361,P=0.003; F=32.371,P=0.005; F=36.734,P=0.021; F=48.336,P=0.023).The blood uric acid levels were greater in model group,Juanbilijieqing group and allopurinol group compared with control group at 1,7 and 14 days after the beginning of the drug intervention(P=0.000,P=0.001,P=0.001; P=0.001,P=0.001,P=0.002; P=0.001,P=0.000,P=0.001).The blood uric acid levels were less in Juanbilijieqing group and allopurinol group compared with model group at 7 and 14 days after the beginning of the drug intervention(P=0.011,P=0.010; P=0.014,P=0.002).The blood uric acid levels were less in control group,Juanbilijieqing group and allopurinol group compared with model group at 21 days after the beginning of the drug intervention(P=0.001,P=0.012,P=0.011).There was no statistical difference in the blood uric acid levels between the paired groups at other timepoints.There was statistical difference in serum XOD activity between the 4 groups at different timepoints after the beginning of the drug intervention(F=45.721,P=0.001; F=50.634,P=0.002; F=49.448,P=0.013; F=63.124,P=0.027).The serum XOD activity was greater in model group,Juanbilijieqing group and allopurinol group compared with control group at 1,7 and 14 days after the beginning of the drug intervention(P=0.002,P=0.001,P=0.001; P=0.001,P=0.000,P=0.001; P=0.001,P=0.001,P=0.000).The serum XOD activity was less in Juanbilijieqing group and allopurinol group compared with model group at 7 and 14 days after the beginning of the drug intervention(P=0.001,P=0.013; P=0.002,P=0.015).The serum XOD activity was less in control group,Juanbilijieqing group and allopurinol group compared with model group at 21 days after the beginning of the drug intervention(P=0.001,P=0.013,P=0.017).There was no statistical difference in serum XOD activity between the paired groups at other timepoints.There was statistical difference in the number of blood vessels in synovium between the 4 groups at different timepoints after the beginning of the drug intervention(F=24.772,P=0.032; F=33.176,P=0.021; F=32.672,P=0.003; F=44.351,P=0.000).The number of blood vessels in synovium was more in model group,Juanbilijieqing group and allopurinol group compared with control group at 1,7,14 and 21 days after the beginning of the drug intervention(P=0.002,P=0.001,P=0.001; P=0.001,P=0.001,P=0.002; P=0.001,P=0.000,P=0.001; P=0.001,P=0.003,P=0.002).The number of blood vessels in synovium was more in Juanbilijieqing group and allopurinol group compared with model group at 7,14 and 21 days after the beginning of the drug intervention(P=0.001,P=0.013; P=0.014,P=0.011; P=0.001,P=0.012).There was no statistical difference in the number of blood vessels in synovium between the paired groups at other timepoints.There was statistical difference in the number of neutrophils in synovium between the 4 groups at different timepoints after the beginning of the drug intervention(F=32.347,P=0.001; F=43.561,P=0.001; F=42.361,P=0.014; F=51.745,P=0.011).The number of neutrophils in synovium was more in model group,Juanbilijieqing group and allopurinol group compared with control group at 1,7 and 14 days after the beginning of the drug intervention(P=0.001,P=0.002,P=0.001; P=0.001,P=0.001,P=0.002; P=0.002,P=0.001,P=0.001).The number of neutrophils in synovium was less in Juanbilijieqing group and allopurinol group compared with model group at 7 and 14 days after the beginning of the drug intervention(P=0.017,P=0.014; P=0.012,P=0.014).The number of neutrophils in synovium was less in control group,Juanbilijieqing group and allopurinol group compared with model group at 21 days after the beginning of the drug intervention(P=0.001,P=0.013,P=0.017).There was no statistical difference in the number of neutrophils in synovium between the paired groups at other timepoints.Conclusion:Juanbilijieqing Fang can effectively reduce the blood uric acid levels and relieve the clinical symptoms of acute gouty arthritis models through decreasing the activity of XOD,and it is similar to allopurinol in the curative effect.

参考文献/References:

[1] 熊辉,曲良烨,向黎黎,等.痛风性关节炎湿热证病证结合模型的建立[J].中医正骨,2014,26(3):14-20.
[2] 尤力都孜·买买提,热比姑丽·伊斯拉木,阿布都吉力力·阿布都艾尼.痛风及其动物模型研究初探[J].中药药理与临床,2012,28(3):172-173.
[3] 齐新宇,董大立,熊辉,等.蠲痹历节清方治疗急性痛风性关节炎湿热证疗效观察[J].湖南中医药大学学报,2014,34(5):42-44.
[4] 齐新宇.蠲痹历节清方对实验性鸡痛风模型血尿酸代谢的影响[D].长沙:湖南中医药大学,2014.
[5] 齐新宇,向黎黎,熊辉,等.基于高蛋白饮食环境下痛风模型的量效研究[J].中医正骨,2015,27(2):1-6.
[6] 朱小甫,吴旭锦,郑义华.高钙高蛋白日粮复制禽痛风病例研究[J].黑龙江畜牧兽医,2013,21(6):36-37.
[7] 任予勇.实验性鸡高尿酸血症模型的建立[J].淮北职业技术学院学报,2013,12(5):133-135.
[8] 黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,9(9):1069-1072.
[9] 匡红艳,程太平,胡建兵,等.鸡持续高尿酸血症模型的制备方法研究[J].四川动物,2008,27(4):554-558.
[10] 苏友新,陈伟宏,王和鸣,等.高尿酸血症及痛风性关节炎的动物模型研究概况[J].中国骨伤,2004,17(7):444-445.
[11] 楼秀余.人类痛风病与UOX基因沉默相关的研究[J].中外医学研究,2012,10(29):154-155.
[12] 孔悦,张冰,刘小青,等.黄嘌呤氧化酶活性在高尿酸血症动物模型中的变化[J].北京中医药大学学报,2004,27(6):38-40.
[13] 齐万虎,蒋企洲,蒋建勤.黄瑞香中化学成分抑制黄嘌呤氧化酶活性及其机制研究[J].中国实验方剂学杂志,2014,20(5):141-144.
[14] 熊辉,姜国勇,胡楠,等.痛风性关节炎中医证素及证型的聚类研究[J].中医正骨,2011,23(11):26-28.
[15] 吴小芳,刘书珍.清热泻湿汤治疗急性痛风性关节炎60例观察与护理[J].实用中医药杂志,2014,30(4):278.
[16] 严立平,周正球.清热利湿方治疗急性痛风性关节炎38例临床观察[J].吉林中医药,2012,32(6):600-601.

相似文献/References:

[1]邓伟哲,王宏晶,王宇恒,等.微创针刀镜治疗难治性膝关节类风湿关节炎[J].中医正骨,2015,27(11):42.
[2]李艺彬,朱勇,吴昭克.关节镜下多入路全关节滑膜清理术治疗肘关节类风湿关节炎[J].中医正骨,2015,27(11):56.
[3]胡钢锋,肖鲁伟,童培建.关节液代谢组学在类风湿关节炎诊断 及寒热证候分型中的应用[J].中医正骨,2015,27(01):5.
　HU Gangfeng,XIAO Luwei,TONG Peijian.Application of joint fluid metabolomics to diagnosis and COLD-HEAT SYMPTOM COMPLEX typing in patients with rheumatoid arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):5.
[4]熊辉,曲良烨,向黎黎,等.痛风性关节炎湿热证病证结合模型的建立[J].中医正骨,2014,26(03):14.
　Xiong Hui*,Qu Liangye,Xiang Lili,et al.A rat model of gouty arthritis combined with dampness-heat syndrome[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(03):14.
[5]孙克民,王平,卢启贵,等.关节镜下滑膜切除术联合中药口服 治疗早期膝关节类风湿关节炎[J].中医正骨,2013,25(09):39.
[6]李峰,郭艳幸,曹向阳,等.贯叶连翘灌胃联合光动力疗法对类风湿关节炎白兔膝关节滑膜组织病理形态的影响[J].中医正骨,2014,26(09):6.
　Li Feng*,Guo Yanxing,Cao Xiangyang,et al.Effect of intragastric administration of hypericum perforatum combined with photodynamic therapy on the pathological morphology of synovial tissue of knee joints in rabbits with rheumatoid arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(03):6.
[7]严超,刘喜德,张金禄,等.蜂针疗法治疗膝骨性关节炎的护理[J].中医正骨,2013,25(01):74.
[8]王笑青,张永红,侯宏理,等.顽痹清丸联合非甾体抗炎药及改善病情的抗风湿药治疗湿热痹阻型类风湿关节炎[J].中医正骨,2013,25(03):41.
　WANG Xiao-qing*,ZHANG Yong-hong,HOU Hong-li,et al.Curative effect and safety of WANBIQING pills combined with nonsteroidal antiinflammatory drugs and disease modifying antirheumatic drugs in the treatment of rheumatoid arthritis of dampness-heat stagnation type[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2013,25(03):41.
[9]黄建武,黄建华,林爱丽.扶中清痹通络汤联合益赛普对活动期类风湿关节炎患者肿瘤坏死因子-α及血管内皮生长因子血清含量的影响[J].中医正骨,2013,25(05):9.
　HUANG Jian-wu*,HUANG Jian-hua,LIN Ai-li.*.Effect of FUZHONG QINGBI TONGLUO decoction combined with etanercept on the serum contents of tumor necrosis factor-alpha and vascular endothelial growth factor in patients with active rheumatoid arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2013,25(03):9.
[10]赫军,李丽华,王相奇,等.痛风四妙汤加味治疗痛风性关节炎[J].中医正骨,2013,25(05):56.
[11]齐新宇,向黎黎,熊辉,等.基于高蛋白饮食建立鸡痛风性关节炎模型的量效关系研究[J].中医正骨,2015,27(02):1.
　QI Xinyu,XIANG Lili,XIONG Hui,et al.A dose-effect relationship study of gouty arthritis chicken model built by high-protein diet[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):1.
[12]陈鹏,郭文韬,龙美兵,等.退癀消肿汤联合洛索洛芬钠片治疗急性痛风性关节炎的临床研究[J].中医正骨,2016,28(08):19.
　CHEN Peng,GUO Wentao,LONG Meibing,et al.Clinical study on Tuihuang Xiaozhong Tang(退癀消肿汤)combined with loxoprofen sodium tablets for treatment of acute gouty arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2016,28(03):19.
[13]孙瑞,郭爱菊,杜梅红,等.能谱CT尿酸基(钙 图像在慢性痛风性关节炎诊断中的应用价值[J].中医正骨,2017,29(03):30.
[14]闵星星,王鹏鹏,刘佳欢,等.第1跖趾关节痛风性关节炎的手术治疗进展[J].中医正骨,2018,30(01):47.
[15]郭玉星,熊辉,周彪,等.Nod样受体家族含pyrin结构域蛋白3 和嘌呤能离子通道型受体7在急性痛风性 关节炎发病中的作用研究进展[J].中医正骨,2018,30(08):39.
[16]蒋恩宇,孙波.中药外敷法治疗急性痛风性关节炎的研究进展[J].中医正骨,2019,31(04):56.
[17]徐艳玲,杜献丽,杜敏,等.痛风性关节炎的延续护理[J].中医正骨,2020,32(05):74.
[18]陈兆军,梁欢,马玉峰,等.痛点超声电导透射清热利湿方药治疗急性痛风性关节炎湿热瘀结证[J].中医正骨,2021,33(12):48.
[19]邓志军,杨文龙,李典,等.杨凤云教授治疗旴江流域痛风性关节炎的经验[J].中医正骨,2023,35(05):64.

备注/Memo

备注/Memo:
2015-01-14收稿 2015-02-06修回
基金项目:湖南省自然科学基金(13JJ3102); 湖南省中医药管理局项目(201411)
通讯作者:熊辉 E-mail:xh_hn@sina.com
更新日期/Last Update: 2015-03-30