[1]赵佳盛,张耘.桃红四物汤治疗兔早期筋膜间隔综合征的 疗效观察及作用机制研究[J].中医正骨,2015,27(04):1-7.
　ZHAO Jiasheng,ZHANG Yun.Experimental research on curative effect and mechanism of action of Taohong Siwu Tang(桃红四物汤)for treatment of early compartment syndromes in rabbits[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):1-7.
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桃红四物汤治疗兔早期筋膜间隔综合征的 疗效观察及作用机制研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期数:
2015年04期
页码:
1-7
栏目:
基础研究
出版日期:
2015-04-30

文章信息/Info

Title:
Experimental research on curative effect and mechanism of action of Taohong Siwu Tang(桃红四物汤)for treatment of early compartment syndromes in rabbits
作者:
赵佳盛1; 张耘2
1.浙江省温岭市中医院,浙江 温岭 317502;
2.河南省洛阳正骨医院/河南省骨科医院,河南 洛阳 471002
Author(s):
ZHAO Jiasheng1; ZHANG Yun2
1.Wenling Hospital of Traditional Chinese Medicine,Wenling 317502,Zhejiang,China
2.Luoyang Orthopedic-Traumatological Hospital,Luoyang 471002,Henan,China
关键词:
筋膜间隔综合征;  桃红四物汤;  血液流变学;  动物实验
Keywords:
compartment syndromes;  Taohong Siwu Tang;  hemorheology;  animal experimentation
摘要:
目的:探讨桃红四物汤治疗早期筋膜间隔综合征的疗效及作用机制。方法:采用气压止血带压迫法将20只成年雄性新西兰大耳白兔制成后肢筋膜间隔综合征模型,造模后将20只兔子随机分为药物组和模型组,每组10只。药物组动物以桃红四物汤灌胃,每次7 mL; 模型组以等量生理盐水灌胃; 每天2次,连续1周。分别于造模前、造模后12 h、1 d、2 d、4 d、7 d观察动物的一般情况、小腿肿胀度、胫前间室内压及血液流变学指标。造模后7 d观察完成之后处死所有动物,取造模侧小腿胫前肌及腓深神经标本在光镜下检查。结果:①一般情况。在实验过程中模型组动物精神不振,饮食减少,大便正常,1只动物死亡; 药物组动物精神、饮食尚可,大便稀溏,1只动物死亡。②小腿肿胀度。造模前后不同时间造模侧小腿肿胀度的差异有统计学意义,即存在时间效应(F=244.260,P=0.000)。2组造模侧小腿肿胀度比较总体上有统计学意义,即存在分组效应(F=128.651,P=0.000); 除造模前和造模后12 h时外,其余各时点模型组的造模侧小腿肿胀度均高于药物组。时间因素与分组因素存在交互效应(F=39.453,P=0.000)。③胫前间室内压。造模前后不同时间造模侧胫前间室内压的差异有统计学意义,即存在时间效应(F=317.971,P=0.000)。2组胫前间室内压比较总体上有统计学意义,即存在分组效应(F=72.285,P=0.000); 除造模前和造模后12 h时外,其余各时点模型组的胫前间室内压均高于药物组。时间因素与分组因素存在交互效应(F=31.071,P=0.000)。④血液流变学指标。造模前后不同时间全血黏度(200/S、30/S、5/S)的差异有统计学意义,即存在时间效应(F=420.316,P=0.000; F=977.477,P=0.000; F=3 283.000,P=0.000)。2组全血黏度(200/S、30/S、5/S)比较总体上有统计学意义,即存在分组效应(F=87.665,P=0.000; F=166.701,P=0.000; F=624.171,P=0.000); 除造模前和造模后12 h时外,其余各时点模型组的全血黏度(200/S、30/S、5/S)均高于药物组。时间因素与分组因素存在交互效应(F=87.611,P=0.000; F=204.266,P=0.000; F=744.771,P=0.000)。造模前后不同时间血浆黏度(plasma viscosity,ηp)的差异有统计学意义,即存在时间效应(F=214.357,P=0.000)。2组ηp比较总体上有统计学意义,即存在分组效应(F=14.874,P=0.000); 除造模前和造模后12 h时外,其余各时点模型组的ηp均高于药物组。时间因素与分组因素存在交互效应(F=70.651,P=0.000)。造模前后不同时间血细胞比容(hematocrit,HCT)的差异有统计学意义,即存在时间效应(F=137.992,P=0.000)。2组HCT比较总体上有统计学意义,即存在分组效应(F=21.696,P=0.000); 除造模前和造模后12 h时外,其余各时点模型组的HCT均高于药物组。时间因素与分组因素存在交互效应(F=109.939,P=0.000)。造模前后不同时间红细胞沉降率(erythrocyte sedimentation rate,ESR)的差异有统计学意义,即存在时间效应(F=71.738,P=0.000)。2组ESR比较总体上有统计学意义,即存在分组效应(F=12.227,P=0.003); 除造模前和造模后12 h时外,其余各时点模型组的ESR均高于药物组。时间因素与分组因素存在交互效应(F=44.082,P=0.000)。⑤肌肉及神经组织形态。模型组造模侧胫前肌肌纤维严重萎缩、坏死,呈玻璃样变性,横纹模糊不清,组织间质纤维化; 腓深神经纤维变性、水肿,核固缩、减少或消失,神经束膜增厚。药物组造模侧胫前肌肌纤维萎缩、横纹模糊,组织间隙增宽,可见少量炎性细胞浸润; 腓深神经纤维水肿,少量变性,神经束膜增厚。结论:桃红四物汤对于早期筋膜间隔综合征具有一定的防治作用,其作用机制可能是通过降低血液流变学指标,改善血液及微循环状态。
Abstract:
Objective:To study the curative effect and mechanism of action of Taohong Siwu Tang(桃红四物汤)for treatment of early compartment syndromes in rabbits.Methods:Twenty adult male big-eared New Zealand rabbits were made into compartment syndromes models in posterior limbs through compression with pneumatic tourniquet,and then the rabbits were randomly divided into drug group and model group,10 cases in each group.The rabbits in drug group were intragastric administrated with Taohong Siwu Tang(7 ml at a time); while the others in model group were intragastric administrated with the same dose of normal saline,twice a day for 1 consecutive week.The general state of health,degree of leg swelling,anterior compartment pressure and hemorheology indexes of rabbits were observed before the modeling and at 12 hrs and 1,2,4 and 7 days after the modeling respectively.All the rabbits were executed at 7 days after the modeling,and their anterior tibial muscles and deep peroneal nerves were fetched out and sectioned for inspection under the light microscope.Results:During the experiment,the rabbits in model group presented with depressed spirit,decreased eating and normal stool,and 1 rabbit died.The rabbits in drug group presented with normal spirit,normal eating and sloppy stool,and 1 rabbit died.There was statistical difference in leg swelling degree between different timepoints,in other words,there was time effect(F=244.260,P=0.000).There was statistical difference in leg swelling degree between the two groups in general,in other words,there was group effect(F=128.651,P=0.000).The leg swelling degree of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=39.453,P=0.000).There was statistical difference in anterior compartment pressure between different timepoints,in other words,there was time effect(F=317.971,P=0.000).There was statistical difference in anterior compartment pressure between the two groups in general,in other words,there was group effect(F=72.285,P=0.000).The anterior compartment pressure of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=31.071,P=0.000).There was statistical difference in blood viscosity(ηb)(200/S,30/S,5/S)between different timepoints,in other words,there was time effect(F=420.316,P=0.000; F=977.477,P=0.000; F=3 283.000,P=0.000).There was statistical difference in ηb(200/S,30/S,5/S)between the two groups in general,in other words,there was group effect(F=87.665,P=0.000; F=166.701,P=0.000; F=624.171,P=0.000).The ηb(200/S,30/S,5/S)of model group were higher than those of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=87.611,P=0.000; F=204.266,P=0.000; F=744.771,P=0.000).There was statistical difference in plasma viscosity(ηp)between different timepoints,in other words,there was time effect(F=214.357,P=0.000).There was statistical difference in ηp between the two groups in general,in other words,there was group effect(F=14.874,P=0.000).The ηp of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=70.651,P=0.000).There was statistical difference in hematocrit(HCT)between different timepoints,in other words,there was time effect(F=137.992,P=0.000).There was statistical difference in HCT between the two groups in general,in other words,there was group effect(F=21.696,P=0.000).The HCT of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=109.939,P=0.000).There was statistical difference in erythrocyte sedimentation rate(ESR)between different timepoints,in other words,there was time effect(F=71.738,P=0.000).There was statistical difference in ESR between the two groups in general,in other words,there was group effect(F=12.227,P=0.003).The ESR of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=44.082,P=0.000).Severe atrophy,necrosis,hyaline degeneration,indefinite transverse striation and interstitial fibrosis were found in pretibial muscle fibers of rabbits in model group.Deep peroneal nerve fibres presented with(1)degeneration,(2)edema,(3)pycnosis,decrease or disappearance of nucleus and(4)thickened perineurium were also found in model group.Atrophy,indefinite transverse striation,dilated tissue spaces and a few inflammatory cells were found in pretibial muscle fibers of rabbits in drug group.Edema,a little degeneration and thickened perineurium in deep peroneal nerve fibres were also found in model group.Conclusion:Taohong Siwu Tang has preventive and therapeutic effect on early compartment syndromes.It can improve the state of blood and microcirculation through lowering the levels of hemorheology indexes,which may be the mechanisms of action for treatment of early compartment syndromes.

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备注/Memo

备注/Memo:
2014-11-20收稿 2015-01-12修回
基金项目:河南省省属科研单位社会公益项目预研专项资金项目
更新日期/Last Update: 2015-04-30