[1]吴权,李启运,王素英,等.活血、温经、补益肝肾类中药对膝骨关节炎兔关节软骨形态的影响[J].中医正骨,2014,26(06):15-18.
　Wu Quan*,Li Qiyun,Wang Suying,et al.Effect of three kinds of traditional Chinese drug including activating blood agents,warming meridians agents and reinforcing liver&kidney agents on articular cartilage morphology in rabbits with knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(06):15-18.
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活血、温经、补益肝肾类中药对膝骨关节炎兔关节软骨形态的 影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年06期
页码:
15-18
栏目:
基础研究
出版日期:
2014-06-30

文章信息/Info

Title:
Effect of three kinds of traditional Chinese drug including activating blood agents,warming meridians agents and reinforcing liver&kidney agents on articular cartilage morphology in rabbits with knee osteoarthritis
作者:
吴权1; 李启运1; 王素英2; 龙树明1; 柯高峰3
1.浙江省宁波市中医院,浙江 宁波 315010; 2.浙江省宁波市病理中心, 浙江 宁波 315031; 3.浙江中医药大学,浙江 杭州 310053
Author(s):
Wu Quan*; Li Qiyun; Wang Suying; Long Shuming; Ke Gaofeng.*
Ningbo Hospital of Traditional Chinese Medicine,Ningbo 315010,Zhejiang,China
关键词:
骨关节炎; 膝 软骨; 关节 活血 温经 补益肝肾 动物实验
Keywords:
Osteoarthritis; knee;  Cartilage; articular;  Activating blood;  Warming meridians;  Reinforcing liver&; kidney;  Animal experimentation
摘要:
目的:观察活血、温经及补益肝肾3类中药对膝骨关节炎兔关节软骨形态的影响。方法:将62只新西兰白兔随机分为6组,A组12只,B、C、D、E和F组各10只。采用关节制动法对B、C、D、E和F组动物左后腿进行膝骨关节炎造模。造模成功后进行药物干预,A、B组以10 mL蒸馏水灌胃,C组以10 mL丹参和当归药液灌胃,D组以10 mL桂枝和防风药液灌胃,E组以10 mL杜仲和怀牛膝药液灌胃,F组以10 mL金乌骨通胶囊水溶液灌胃。各组动物均在每天上午10时给药1次。分别于药物干预21 d和42 d后分2批处死动物,取左后腿股骨外髁关节软骨及软骨下骨,制成组织切片后分别进行HE染色和阿利辛蓝-过碘酸雪夫氏染色,采用Mankin软骨组织学评分法进行评分。结果:①动物一般情况。造模结束时,B、C、D、E和F组动物膝关节肿胀,屈伸活动不利,行走呈跨越步态。造模期间共有9只动物因拒绝进食死亡。②Mankin评分。药物干预后21 d,各组动物膝关节软骨Mankin评分比较,差异有统计学意义[(0.67±0.82)分,(13.00±1.63)分,(4.75±2.22)分,(6.75±0.96)分,(8.25±1.26)分,(9.75±2.36)分,F=34.904,P=0.000]。组间两两比较,A组评分小于B、C、D、E和F组(P=0.000,P=0.000,P=0.000,P=0.000,P=0.009); B组大于C、D、E和F组(P=0.001,P=0.000,P=0.000,P=0.000); C组小于E组和F组(P=0.005,P=0.000); D组小于F组(P=0.015); 其余各组间两两比较,差异均无统计学意义。药物干预后42 d,各组动物膝关节软骨Mankin评分比较,差异有统计学意义[(0.67±1.03)分,(13.25±1.50)分,(3.00±0.81)分,(4.50±1.73)分,(4.00±1.10)分,(4.75±1.71)分,F=44.868,P=0.000]。组间两两比较,A组评分小于B、C、D、E和F组(P=0.000,P=0.014,P=0.000,P=0.001,P=0.000); B组大于C、D、E和F组(P=0.000,P=0.000,P=0.000,P=0.000); 其余各组间两两比较,差异均无统计学意义。E组和F组干预后42 d的膝关节软骨Mankin评分小于干预后21 d的膝关节软骨Mankin评分(t=5.112,P=0.001; t=3.532,P=0.017),其余各组药物干预后21 d和42 d膝关节软骨Mankin评分比较,差异均无统计学意义。结论:活血、温经及补益肝肾3类中药均能改善膝骨关节炎兔膝关节软骨形态,达到保护软骨、延缓软骨退变的目的,但不能阻断和逆转软骨退变的进程。
Abstract:
Objective:To observe the effect of three kinds of traditional Chinese drug(TCD)including activating blood agents,warming meridians agents and reinforcing liver&kidney agents on articular cartilage morphology in rabbits with knee osteoarthritis(KOA).Methods:Sixty-two New Zealand white rabbits were randomly divided into 6 groups,12 cases in group A and 10 cases in each of the rest groups.The rabbits in group B,C,D,E and F were administrated with immobilization in the left hind legs to build models of KOA.Then,the rabbits in group A and B were intragastric administrated with distilled water(10 mL),while the rabbits in group C,D,E and F were intragastric administrated with solution of salvia miltiorrhiza and angelica(10mL),solution of cassia twig and saposhnikovia divaricata(10 mL),solution of eucommia ulmoides and bidentata blume(10 mL)and JINWU GUTONG solution(10 mL)respectively.The rabbits in each group were dosed with drug once a day at 10 am.The rabbits were executed in 2 batches at 3 and 6 weeks after the beginning of the drug intervention,and their lateral condyle articulars cartilago and subchondral bone in the left hind leg were fetched out.Their histologic sections were received HE staining and Alcian blue-periodic acid Schiff staining respectively and were evaluated according to Mankin cartilage histological score.Results:After the modeling,the knee of the rabbits in group B,C,D,E and F were swollen and were unfavourable for flexion.The rabbits showed steppage gait.Nine rabbits died of hunger strike during the modeling period.There was statistical difference in Mankin scores between the 6 groups at 21 days after the beginning of the drug intervention(0.67+/-0.82,13.00+/-1.63,4.75+/-2.22,6.75+/-0.96,8.25+/-1.26,9.75+/-2.36 points,F=34.904,P=0.000).Further pairwise comparison showed that the score of group A was less than those of group B,C,D,E and F(P=0.000,P=0.000,P=0.000,P=0.000,P=0.009),the score of group B was greater than those of group C,D,E and F(P=0.001,P=0.000,P=0.000,P=0.000),the score of group C was less than those of group E and F(P=0.005,P=0.000),and the score of group D was less than that of group F(P=0.015),while there was no statistical difference in Mankin scores between the rest paired groups.There was statistical difference in Mankin scores between the 6 groups at 42 days after the beginning of the drug intervention(0.67+/-1.03,13.25+/-1.50,3.00+/-0.81,4.50+/-1.73,4.00+/-1.10,4.75+/-1.71 points,F=44.868,P=0.000).Further pairwise comparison showed that the score of group A was less than those of group B,C,D,E and F(P=0.000,P=0.014,P=0.000,P=0.001,P=0.000),the score of group B was greater than those of group C,D,E and F(P=0.000,P=0.000,P=0.000,P=0.000),while there was no statistical difference in the scores between the rest paired groups.The Mankin scores of group E and F were less at 42 days compared to 21 days after the beginning of the drug intervention(t=5.112,P=0.001; t=3.532,P=0.017),while there was no statistical difference in the scores between the two time points for the other groups.Conclusion:All the three kinds of TCD,including activating blood agents,warming meridians agents and reinforcing liver&kidney agents,can ameliorate articular cartilage morphology in rabbits with KOA,so as to protect the cartilage and delay cartilage degeneration.However,it can't interdict and reverse the process of cartilage degeneration.

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