[1]程真真,唐洪涛,程春生.加味黄芪桂枝五物汤联合甲钴胺治疗周围神经损伤的实验研究[J].中医正骨,2014,26(06):10-14.
　Cheng Zhenzhen*,Tang Hongtao,Cheng Chunsheng.*.Effect of oral application of JIAWEI HUANGQI GUIZHI WUWU decoction and mecobalamin on peripheral nerve injuries[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(06):10-14.
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加味黄芪桂枝五物汤联合甲钴胺治疗周围神经损伤的实验研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年06期
页码:
10-14
栏目:
基础研究
出版日期:
2014-06-30

文章信息/Info

Title:
Effect of oral application of JIAWEI HUANGQI GUIZHI WUWU decoction and mecobalamin on peripheral nerve injuries
作者:
程真真; 唐洪涛; 程春生
河南省洛阳正骨医院,河南 洛阳 471002
Author(s):
Cheng Zhenzhen*; Tang Hongtao; Cheng Chunsheng.*
Luoyang Orthopedic-Traumatological Hospital,Luoyang 471002,Henan,China
关键词:
周围神经损伤 黄芪桂枝五物汤 甲钴胺 动物实验
Keywords:
Peripheral nerve injuries;  HUANGQI GUIZHI WUWU decoction;  Mecobalamin;  Animal experimentation
摘要:
目的:探讨加味黄芪桂枝五物汤联合甲钴胺治疗周围神经损伤的疗效。方法:将54只SD大鼠随机分为3组,每组18只,均采用左侧坐骨神经切断缝合建立坐骨神经损伤模型。造模后第3天,A组大鼠按1 mL·100 g-1以加味黄芪桂枝五物汤灌胃,每天1次,连续4周; 同时按1 mL·100 g-1以甲钴胺和生理盐水制成的混悬液(0.05 mg·mL-1)灌胃,每天1次,连续4周。B组以甲钴胺和生理盐水制成的混悬液灌胃,用法、用量同A组。C组按1 mL·100 g-1以生理盐水灌胃,每天1次,连续4周。观察各组大鼠的一般状态,并测定坐骨神经功能指数、神经传导速度恢复率、复合肌肉诱发电位振幅恢复率、小腿三头肌湿重恢复率及神经轴突恢复率。结果:①一般状态。造模后1 d所有大鼠均蜷缩伤肢,3 d后跛行,切口均无感染。药物干预后第2周,3组大鼠左后小腿肌肉均有不同程度的萎缩,C组较A、B组更为明显,且C组部分大鼠出现跗关节肿胀和皮肤溃疡,多在第4周左右自行愈合。自药物干预后第4周开始,大鼠跛行逐渐改善,A组大鼠毛发光亮,运动灵活,爬行速度快; B组大鼠毛发较暗,运动较灵活,爬行速度较快; C组毛发暗淡,活动较差,反应较迟钝。②坐骨神经功能指数。药物干预后第1周、第2周、第3周及第4周坐骨神经功能指数的差异有统计学意义,即存在时间效应[(-75.61±8.20),(-62.89±7.36),(-30.89±6.14),(-18.33±6.88);(-75.39±7.70),(-60.67±7.53),(-33.11±6.43),(-24.11±7.32);(-80.33±6.78),(-65.67±7.88),(-55.22±7.26),(-29.56±7.88); F=2.015,P=0.000]。3组大鼠坐骨神经功能指数的组间差异总体上有统计学意义,即存在分组效应(F=82.434,P=0.000); 药物干预后第3周和第4周A组的坐骨神经功能指数均高于B组和C组(P=0.004,P=0.000; P=0.023,P=0.000)。时间因素和分组因素之间存在交互效应(F=10.928,P=0.000)。③神经传导速度恢复率。3组大鼠神经传导速度恢复率比较,差异有统计学意义[(66.39±11.77)%,(53.72±14.40)%,(44.72±11.21)%,F=13.569,P=0.000]。A组大鼠神经传导速度恢复率高于B组和C组(P=0.004,P=0.000); B组高于C组(P=0.036)。④复合肌肉诱发电位振幅恢复率。3组大鼠复合肌肉诱发电位振幅恢复率比较,差异有统计学意义[(63.22±14.29)%,(45.06±11.72)%,(32.44±7.82)%,F=32.117,P=0.000]。A组复合肌肉动作电位振幅恢复率高于B组和C组(P=0.000,P=0.000); B组优高于C组(P=0.002)。⑤小腿三头肌湿重恢复率。3组大鼠小腿三头肌湿重恢复率比较,差异有统计学意义[(78.56±7.01)%,(65.78±8.19)%,(58.28±10.84)%,F=29.940,P=0.000]。A组高于B组和C组(P=0.000,P=0.000),B组高于C组(P=0.013)。⑥神经轴突恢复率。3组大鼠神经轴突恢复率比较,差异有统计学意义[(78.28±7.91)%,(69.89±8.08)%,(62.94±10.66)%,F=13.889,P=0.000]。A组高于B组和C组(P=0.006,P=0.000),B组高于C组(P=0.021)。结论:加味黄芪桂枝五物汤联合甲钴胺可有效促进受损周围神经的修复和再生。
Abstract:
Objective:To explore the curative effect of oral application of JIAWEI HUANGQI GUIZHI WUWU decoction and mecobalamin on peripheral nerve injuries.Methods:Fifty-four SD rats were randomly divided into 3 groups,18 rats in each group.The sciatic nerve injuries models were created through transecting and suturing the sciatic nerve at the left hind legs of rats.The rats in group A were intragastric administrated with JIAWEI HUANGQI GUIZHI WUWU decoction(1 mL/100 g)on the 3rd day after modeling,once per day for 4 consecutive weeks.Meanwhile,the rats in group A were intragastric administrated with the mecobalamin solution(0.05 mg/mL,1 mL/100 g),once per day for 4 consecutive weeks.The rats in group B were intragastric administrated with the mecobalamin solution(0.05 mg/mL),which usage and dosage were same as that of group A.The rats in group C were intragastric administrated with normal saline(1 mL/100 once per day for 4 consecutive weeks.After that,the general status of rats in each group were observed,and the sciatic nerve function index(SFI),nerve conduction velocity(NCV)recovery rate,compound muscle evoked potential amplitude recovery rate,wet weight of triceps muscle(WWT)recovery rate and nerve axons recovery rate were all determined.Results:All rats curled their injured legs one day after modeling and claudication were found 3 days after modeling,and no incision infection were found.The various degrees of muscle wasting were found in the left hind legs of the rats in 3 groups on the 2nd week after the beginning of drug intervention.Compared with group A and group B,muscle wasting were more significant in group C.Meanwhile,the hock joints swelling and skin ulcers were found in several rats of group C,and most skin ulcers healed naturally on the 4th week after the beginning of drug intervention.The claudication of rats were improved gradually since the 4th week after the beginning of drug intervention.The rats in group A presented with vivid hair,swift action and fast crawling speed; and the rats in group B presented with dark hair,relative swift action and relative fast crawling; while the rats in group C presented with dim hair and sluggish action.There was statistical difference in SFI between different time points after the beginning of the drug intervention,in other words,there was time effect(-75.61+/-8.20,-62.89+/-7.36,-30.89+/-6.14,-18.33+/-6.88; -75.39+/-7.70,-60.67+/-7.53,-33.11+/-6.43,-24.11+/-7.32; -80.33+/-6.78,-65.67+/-7.88,-55.22+/-7.26,-29.56+/-7.88; F=2.015,P=0.000).There was statistical difference in SFI between the 3 groups in general,in other words,there was grouping effect(F=82.434,P=0.000).The SFI of group A was higher than that of group B and group C on the 3rd and 4th week after the beginning of the drug intervention(P=0.004,P=0.000; P=0.023,P=0.000).There was interaction between time factor and grouping factor(F=10.928,P=0.000).There was statistical difference in NCV recovery rate between the 3 groups(66.39+/-11.77%,53.72+/-14.40%,44.72+/-11.21%,F=13.569,P=0.000).The NCV recovery rate of group A was higher than that of group B and group C(P=0.004,P=0.000),and group B surpassed group C(P=0.036).There was statistical difference in the compound muscle evoked potential amplitude recovery rate between the 3 groups(63.22+/-14.29%,45.06+/-11.72%,32.44+/-7.82%,F=32.117,P=0.000),and group A surpassed group B and group C(P=0.000,P=0.000)and group B surpassed group C(P=0.002).There was statistical difference in the WWT recovery rate between the 3 groups(78.56+/-7.01%,65.78+/-8.19%,58.28+/-10.84%,F=29.940,P=0.000),and group A surpassed group B and group C(P=0.000,P=0.000)and group B surpassed group C(P=0.013).There was statistical difference in the nerve axons recovery rate between the 3 groups(78.28+/-7.91%,69.89+/-8.08%,62.94+/-10.66%,F=13.889,P=0.000),and group A surpassed group B and group C(P=0.006,P=0.000)and group B surpassed group C(P=0.021).Conclusion:The therapy of oral application of JIAWEI HUANGQI GUIZHI WUWU decoction and mecobalamin can effectively promote the recovery and regeneration of injured peripheral nerves.

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更新日期/Last Update: 1900-01-01