[1]潘哲尔,陈乃钱,张纯武,等.受体酪氨酸激酶抑制剂对兔OA模型软骨 细胞NO的表达及软骨组织形态的影响[J].中医正骨,2011,23(05):19-23.
 PAN Zhe-er*,CHEN Nai-qian,ZHANG Chun-wu,et al.The effects of genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(05):19-23.
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受体酪氨酸激酶抑制剂对兔OA模型软骨 细胞NO的表达及软骨组织形态的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期数:
2011年05期
页码:
19-23
栏目:
基础研究
出版日期:
2011-05-30

文章信息/Info

Title:
The effects of genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats
作者:
潘哲尔陈乃钱张纯武李驰黄小敬叶澄宇
温州医学院附属第一医院,浙江 温州 325000
Author(s):
PAN Zhe-er*CHEN Nai-qianZHANG Chun-wuet al.*
The First Affilatet Hospieal of Wenzhou Medical College,Ruian 325000,Zhejiang,China
关键词:
骨关节炎 软骨细胞 受体酪氨酸激酶抑制剂 一氧化氮
Keywords:
osteoarthritis chondrocyte recePtor tyrosine kinase inhibitor nitric oxide
摘要:
目的:探讨受体酪氨酸激酶抑制剂genistein对兔OA模型软骨细胞NO的表达及软骨组织形态的影响,并探讨其可能的作用机制。方法:参照Hulth法建立兔骨关节炎模型,予以genistein干预后,分别按照0.1 mg·kg-1、0.2 mg·kg-1、0.3 mg·kg-1、0.4 mg·kg-1、0.5 mg·kg-1的给药剂量进行分组,行手术侧关节腔内注射,通过测定各组亚硝酸盐含量检测关节腔内NO的表达水平,同时进行软骨组织形态学检查。结果:0.3 mg·kg-1、0.4 mg·kg-1、0.5 mg·kg-1浓度genistein干预组关节腔内NO的表达水平明显低于对照组,差异具有统计学意义(P<0.05); 对软骨组织行H.E/SOFG染色显示,在造模后第4周、第8周与第12周时,genistein干预组与对照组相比,组织损伤均明显减轻,Mankin评分均明显下降,差异具有统计学意义(P<0.05)。结论:受体酪氨酸激酶抑制剂genistein在体内能减少骨关节炎软骨细胞NO的表达,对于延迟关节软骨在组织形态水平的退变是有效的, 但是尚不能最终阻止软骨退变发生发展的进程。
Abstract:
Objective:To investigate the effects of recePtor tyrosine kinase inhibitor genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats, thus try to exPress the Probable mechanisms involved.Methods:According to Hulth method,we built rat OA model and the exPression levels of NO was detected by Griess method and the change of ultra-microstructure of cartilage was observed.Results:ComPared with control grouP, less levels of NO were detected after the intervention of genistein(0.3 mg·kg-1、0.4 mg·kg-1 and 0.5 mg·kg-1)(P<0.05). At 4、8 and 12 weeks after oPeration, cartilage sections made and stain with HE/SOFG stain showed less injury, and Mankin score were significantly decreased in intervention grouPs comPared with control grouP(P<0.05).Conclusion:Genistein can reduced the exPression of NO in-vivo. The results of Mankin score and HE/SOFG stain showed that genistein Partly delayed the observation of ultra-microstructure of cartilage exPlored the alleviation of the ultra-microstructure damage of cartilage after injury.

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备注/Memo

备注/Memo:
基金项目:温州市科技局科学研究基金(编号:NO Y20090089)
更新日期/Last Update: 2011-05-30