[1]王超逸,杨敬言,赵余炀,等.站立身高与椎间盘退变因果关系的孟德尔随机化分析[J].中医正骨,2024,36(04):55-61,65.
 WANG Chaoyi,YANG Jingyan,ZHAO Yuyang,et al.The causal relationship between standing height and intervertebral disc degeneration:a mendelian randomization analysis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2024,36(04):55-61,65.
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站立身高与椎间盘退变因果关系的孟德尔随机化分析()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第36卷
期数:
2024年04期
页码:
55-61,65
栏目:
数据库研究
出版日期:
2024-04-20

文章信息/Info

Title:
The causal relationship between standing height and intervertebral disc degeneration:a mendelian randomization analysis
作者:
王超逸杨敬言赵余炀黄仁俊马涉于栋
北京中医药大学第三附属医院,北京 100029
Author(s):
WANG ChaoyiYANG JingyanZHAO YuyangHUANG RenjunMA SheYU Dong
Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China
关键词:
椎间盘退化 站立身高 孟德尔随机化分析 全基因组关联研究
Keywords:
intervertebral disc degeneration standing height Mendelian randomization analysis genome-wide association study
摘要:
目的:探讨站立身高和椎间盘退变的因果关系。方法:从IEU OpenGWAS project数据库获取站立身高的全基因组关联研究(genome-wide association study,GWAS)数据集,筛选符合要求的单核苷酸多态性(single nucleotide polymorphism,SNP)位点作为工具变量; 从FINNGEN数据库中获取关于椎间盘退变的GWAS数据集,从中筛选与工具变量匹配的SNP位点作为结局变量。将工具变量与结局变量导入分析工具R包,采用逆方差加权法(Inverse variance weighted,IVW)、MR-Egger回归、简单中位数法(Simple mode,SM)、加权中值法(Weighted median estimator,WME)、加权中位数法(Weighted mode,WM)进行孟德尔随机化(Mendelian randomization,MR)分析。采用MR-Egger截距检验、Cochran's Q检验、留一法进行敏感性分析。结果:MR分析结果显示,站立身高与椎间盘退变呈正向因果关系[MR-Egger:OR=1.148,95%CI(0.942,1.401),P=0.174; WME:OR=1.220,95%CI(1.086,1.371),P=0.000; IVW:OR=1.113,95%CI(1.011,1.226),P=0.030; SM:OR=1.106,95%CI(0.837,1.462),P=0.479; WM:OR=1.234,95%CI(1.004,1.519),P=0.048]。MR-Egger截距检验结果表明,MR分析结果不存在水平多效性(P=0.726); 异质性检验结果显示,站立身高SNP位点存在异质性(P=0.000); 采用IVW的随机效应模型再次评估,结果显示站立身高与椎间盘退变呈正向因果关系(P=0.000),异质性存在对结果无影响; 留一法检验结果显示,MR分析结果稳定。结论:站立身高与椎间盘退变呈正向因果关系。
Abstract:
Objective:To explore the causal relationship between standing height and intervertebral disc degeneration(IVDD).Methods:The genome-wide association study(GWAS)datasets about standing height was extracted from the IEU OpenGWAS project database,and the eligible single nucleotide polymorphism(SNP)loci were screened as instrumental variables; meanwhile,the GWAS datasets on IVDD was retrieved from the FINNGEN database,and the SNP loci matched with the instrumental variables were selected as the outcome variables.The instrumental variables and outcome variables were imported into an R package,and then a mendelian randomization(MR)analysis was conducted by using Inverse variance weighted(IVW),MR-Egger regression,Simple mode(SM),Weighted median estimator(WME)and Weighted mode(WM),and the sensitivity was examined via the MR-Egger intercept test,Cochran's Q test,and the leave-one-out(LOO)test.Results:The results of MR analysis showed a positive causal relationship between standing height and IVDD(MR-Egger:OR=1.148,95%CI(0.942,1.401),P=0.174; WME:OR=1.220,95%CI(1.086,1.371),P=0.000; IVW:OR=1.113,95%CI(1.011,1.226),P=0.030; SM:OR=1.106,95%CI(0.837,1.462),P=0.479; WM:OR=1.234,95%CI(1.004,1.519),P=0.048).The results of MR-Egger intercept test indicated that there was no horizontal pleiotropy in the MR analysis results(P=0.726).The results of heterogeneity test revealed that there was heterogeneity in SNP loci of standing height(P=0.000).A re-evaluation was performed by employing a random-effects model of IVW,and the results showcased that the standing height had a positive causal relationship with IVDD(P=0.000),suggesting heterogeneity in SNP loci of standing height having no impact on the MR analysis results.Furthermore,the LOO test suggested that the results of MR analysis were stable.Conclusion:Standing height exhibits a positive causal relationship with IVDD.

参考文献/References:

[1] CHEUNG K M C,KARPPINEN J,CHAN D,et al.Prevalence and pattern of lumbar magnetic resonance imaging changes in a population study of one thousand forty-three individuals[J].Spine,2009,34(9):934-940.
[2] ZHENG D K Y,KAWCHUK G N,BUSSIÉRES A E,et al.Trends of low back pain research in older and working-age adults from 1993 to 2023:a bibliometric analysis[J].J Pain Res,2023,16:3325-3341.
[3] ZHAO W,LI Y,CHENG X,et al.The antioxidant glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells[J].Aging,2023,15(23):13693-13709.
[4] OKU N,DEMURA S,TAWARA D,et al.Biomechanical investigation of long spinal fusion models using three-dimensional finite element analysis[J].BMC Musculoskeletal Disorders,2023,24(1):175.
[5] STOTT B,DRISCOLL M.Biomechanical evaluation of the thoracolumbar spine comparing healthy and irregular thoracic and lumbar curvatures[J].Comput Biol Med,2023,160:106982.
[6] NAZARZADEH M,PINHO-GOMES A C,BIDEL Z,et al.Plasma lipids and risk of aortic valve stenosis:a Mendelian randomization study[J].Eur Heart J,2020,41(40):3913-3920.
[7] LAWLOR D A,HARBORD R M,STERNE J A C,et al.Mendelian randomization:using genes as instruments for making causal inferences in epidemiology[J].Stat Med,2008,27(8):1133-1163.
[8] PAPADOPOULOU A,ÅSVOLD B O,BURGESS S,et al.Height,autoimmune thyroid disease,and thyroid cancer:a Mendelian randomization study[J].Thyroid,2023,33(12):1476-1482.

[9] SKRIVANKOVA V W,RICHMOND R C,WOOLF B A R,et al.Strengthening the reporting of observational studies in epidemiology using Mendelian randomization:the STROBE-MR statement[J].JAMA,2021,326(16):1614-1621.
[10] QIU S,ZHENG K,HU Y,et al.Genetic correlation,causal relationship,and shared loci between vitamin D and COVID-19:a genome-wide cross-trait analysis[J].J Med Virol,2023,95(5):e28780.
[11] FRIGOLA J,SABARINATHAN R,MULARONI L,et al.Author correction:reduced mutation rate in exons due to differential mismatch repair[J].Nat Genet,2018,50(8):1196.
[12] BOWDEN J,DAVEY SMITH G,BURGESS S.Mendelian randomization with invalid instruments:effect estimation and bias detection through Egger regression[J].Int J Epidemiol,2015,44(2):512-525.
[13] NUNNEY L.Size matters:height,cell number and a person's risk of cancer[J].Proc Biol Sci,2018,285(1889):20181743.
[14] KERR G J,TO B,WHITE I,et al.Diet-induced obesity leads to behavioral indicators of pain preceding structural joint damage in wild-type mice[J].Arthritis Res Ther,2021,23(1):93.
[15] BHATTACHARYA S,DUBEY D K.Impact of variations in water concentration on the nanomechanical behavior of type I collagen microfibrils in annulus fibrosus[J].J Biomech Eng,2022,144(4):041004.
[16] YANG M,FENG C,ZHANG Y,et al.Autophagy protects nucleus pulposus cells from cyclic mechanical tension induced apoptosis[J].Int J Mol Med,2019,44(2):750-758.
[17] JIA H,LIN X,WANG D,et al.Injectable hydrogel with nucleus pulposus-matched viscoelastic property prevents intervertebral disc degeneration[J].J Orthop Translat,2022,33:162-173.
[18] MIDDENDORF J M,BAROCAS V H.An approach to quantify anisotropic multiaxial failure of the annulus fibrosus[J].J Biomech Eng,2024,146(1):014501.
[19] YING Y,CAI K,CAI X,et al.Recent advances in the repair of degenerative intervertebral disc for preclinical applications[J].Front Bioeng Biotechnol,2023,11:1259731.
[20] VAN DE SANDE M G H,ELEWAUT D.Pathophysiology and immunolgical basis of axial spondyloarthritis[J].Best Pract Res Clin Rheumatol,2023,37(3):101897.
[21] YANG F,MA N,LI S,et al.Tanshinone IIA alleviates early brain injury after subarachnoid hemorrhage in rats by inhibiting the activation of NF-κB/NLRP3 inflammasome[J].Biol Pharm Bull,2024,47(1):279-291.
[22] KRUZICOVA A,CHALUPOVA M,KUZMINOVA G,et al.Effect of novel carboxymethyl cellulose-based dressings on acute wound healing dynamics[J].Vet Med(Praha),2023,68(10):403-411.
[23] CHEN M,DENG J,BAO L,et al.Biomechanical characteristics of a novel interspinous distraction fusion device in the treatment of lumbar degenerative diseases:a finite element analysis[J].BMC Musculoskelet Disord,2023,24(1):944.
[24] ZYOUD T Y T,ABDUL RASHID S N,SUPPIAH S,et al.Estimation of body height from spinal length measurements using post-mortem computed tomographic images[J].Malays J Pathol,2020,42(3):423-431.
[25] ZHENG L D,CAO Y T,YANG Y T,et al.Biomechanical response of lumbar intervertebral disc in daily sitting postures:a poroelastic finite element analysis[J].Comput Methods Biomech Biomed Engin,2023,26(16):1941-1950.
[26] ZHAO J,WANG J,XU H,et al.Intervertebral disk degeneration and bone mineral density:a bidirectional mendelian randomization study[J].Calcif Tissue Int,2024,114(3):228-236.
[27] YUAN S,MIAO Y,RUAN X,et al.Therapeutic role of interleukin-1 receptor antagonist in pancreatic diseases:mendelian randomization study[J].Front Immunol,2023,14:1240754.
[28] HAN Y,ZHANG Y,ZENG X.Assessment of causal associ-ations between uric acid and 25-hydroxyvitamin D levels[J].Front Endocrinol(Lausanne),2022,13:1024675.
[29] LI J,BAI H,QIAO H,et al.Causal effects of COVID-19 on cancer risk:a Mendelian randomization study[J].J Med Virol,2023,95(4):e28722.
[30] DOBRIJEVIC E,VAN ZWIETEN A,KIRYLUK K,et al.Mendelian randomization for nephrologists[J].Kidney Int,2023,104(6):1113-1123.
[31] CORDEIRO C,MUÑOZ-BARUS J I,WASTERLAIN S,et al.Predicting adult stature from metatarsal length in a Portuguese population[J].Forensic Sci Int,2009,193(1/3):131.e1-131.e4.

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备注/Memo

备注/Memo:
通讯作者:于栋 E-mail:yu_dong01@126.com
更新日期/Last Update: 1900-01-01