[1]王文波,董建文,杨振国,等.加味阳和汤对早期膝骨关节炎兔关节软骨的影响[J].中医正骨,2015,27(01):1-4.
　WANG Wenbo,DONG Jianwen,YANG Zhenguo,et al.Effect of Jiawei Yanghe Tang(加味阳和汤)on articular cartilage in rabbits with early knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(01):1-4.
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加味阳和汤对早期膝骨关节炎兔关节软骨的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期数:
2015年01期
页码:
1-4
栏目:
基础研究
出版日期:
2015-01-15

文章信息/Info

Title:
Effect of Jiawei Yanghe Tang(加味阳和汤)on articular cartilage in rabbits with early knee osteoarthritis
作者:
王文波; 董建文; 杨振国; 王树强; 王磊; 刘文斌
山东中医药大学,山东 济南 250355
Author(s):
WANG Wenbo; DONG Jianwen; YANG Zhenguo; WANG Shuqiang; WANG Lei; LIU Wenbin
Shandong University of Traditional Chinese Medicine,Jinan 250355,Shangdong,China
关键词:
骨关节炎; 膝;  软骨; 关节;  加味阳和汤;  动物实验
Keywords:
osteoarthritis; knee;  cartilage; articular;  Jiawei Yanghe Tang;  animal experimentation
摘要:
目的:观察加味阳和汤对早期膝骨关节炎兔关节软骨的影响。方法:将30只6月龄普通级健康新西兰大白兔随机分为空白组、模型组和实验组,每组10只。除空白组外,其余2组动物均按照改良Hulth法对右侧膝关节进行早期膝骨关节炎造模。造模成功后,实验组动物以加味阳和汤灌胃,模型组动物以等量生理盐水灌胃,连续4周。药物干预结束后,对膝关节进行X线检查和解剖学大体观察,并于胫骨平台内侧取关节软骨制作HE染色切片,光镜下观察软骨改变,按照Mankin评分标准进行评分。结果:①X线观察。空白组兔膝关节关节间隙对称,关节面光滑平整,无明显增生; 模型组可见膝关节内侧间隙基本消失,呈内翻畸形,股骨内侧髁变大,胫骨内侧平台不平整,股骨内髁及胫骨内侧平台可见大量增生骨赘; 实验组可见膝关节间隙不平整,内侧间隙变窄,程度较模型组轻,股骨内髁、胫骨内侧平台有少量骨赘形成,但较模型组变化小。②大体观察。按软骨形态退化程度由轻至重分级,空白组Ⅰ级9只、Ⅱ级1只,模型组Ⅱ级3只、Ⅲ级7只,实验组Ⅱ级5只、Ⅲ级5只。3组兔膝关节关节软骨形态比较,空白组最好、实验组次之、模型组最差(R^-空白组=5.90,R^-模型组=21.35,R^-实验组=19.25; χ2=20.524,P=0.000)。③镜下观察。3组兔关节软骨Mankin评分分别为(0.550±0.675)分、(7.950±1.535)分、(4.300±1.337)分,组间比较差异有统计学意义(F=89.233,P=0.000); 空白组评分低于模型组和实验组(P=0.000,P=0.000),模型组高于实验组(P=0.000)。结论:加味阳和汤可抑制早期膝骨关节炎兔关节软骨的退变,这可能是其治疗早期膝骨关节炎的作用机制之一。
Abstract:
Objective:To observe the effect of Jiawei Yanghe Tang(加味阳和汤,JWYHT)on articular cartilage in rabbits with early knee osteoarthritis.Methods:Thirty 6-month-old ordinary healthy New Zealand white rabbits were randomly divided into blank group,model group and experimental group,10 cases in each group.The early knee osteoarthritis models of right knee joint were built by modified Hulth method in rabbits of model group and experimental group.After that,the rabbits in experimental group were intragastric administrated with JWYHT,while rabbits in model group were intragastric administrated with normal saline for consecutive 4 weeks.After drug intervention,X-ray examination and roughly anatomical observation were performed on knee joints.The cartilago articularis were fetched from medial tibial plateau and were sectioned for HE staining.All the samples were observed under the optical microscope and the cartilaginous changes were evaluated according to Mankin Score.Results:Symmetrical knee joint gap and smooth articular surface and no obvious hyperplasia were found in rabbits of blank group.In model group,the medial joint gap almost disappeared and the knee presented with varus deformity; and accrescent medial femoral condyle,rough medial tibial plateau and a large number of osteophytes located at medial femoral condyle and medial tibial plateau were found.In experimental group,the knee joint gap was not smooth and medial knee joint gap became narrow and a few osteophytes were found at medial femoral condyle and medial tibial plateau.In fact,less anatomical changes were found in the experimental group compared to the model group.The cartilage morphology was classified according to its degradation degree,The rabbits belonged to gradeⅠ(9)and gradeⅡ(1)in blank group,gradeⅡ(3)and gradeⅢ(7)in model group,gradeⅡ(5)and gradeⅢ(5)in experimental group.Comparison showed that the blank group was the best,experimental group was good and model group was the worst in knee articular cartilage morphology(R^-blank group=5.90,R^-model group=21.35,R^-experimental group=19.25; χ2=20.524,P=0.000).Mankin scores of cartilago articularis was 0.550+/-0.675,7.950+/-1.535 and 4.300+/-1.337 points respectively,there was statistical difference between the 3 groups(F=89.233,P=0.000).Mankin scores were lower in blank group compared to model group and experimental group(P=0.000, P=0.000),and were higher in model group compared to experimental group(P=0.000).Conclusion:The JWYHT can inhibit the degeneration of cartilago articularis of rabbits with early knee osteoarthritis,so it may be one of the mechanisms of action for treatment of early knee osteoarthritis.

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备注/Memo

备注/Memo:
2014-11-05收稿 2014-11-26修回
基金项目:国家自然科学基金项目(81173286); 济南市科技发展计划项目(201102063)
通讯作者:董建文 E-mail:dongjianwen2006@sina.com
更新日期/Last Update: 2015-01-30