[1]金成鹏,孙绍裘,彭志飞,等.桃红四物汤对大鼠血管吻合模型吻合区Delta-like4表达及血管新生的影响[J].中医正骨,2017,29(12):1-5.
　JIN Chengpeng,SUN Shaoqiu,PENG Zhifei,et al.Effect of Taohong Siwu Tang(桃红四物汤)on Delta-like4 expression and angiogenesis in anastomosed area in vascular anastomose rat models[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(12):1-5.
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桃红四物汤对大鼠血管吻合模型吻合区Delta-like4表达及血管新生的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第29卷
期数:
2017年12期
页码:
1-5
栏目:
基础研究
出版日期:
2017-12-20

文章信息/Info

Title:
Effect of Taohong Siwu Tang(桃红四物汤)on Delta-like4 expression and angiogenesis in anastomosed area in vascular anastomose rat models
作者:
金成鹏; 孙绍裘; 彭志飞; 陈希龙; 曾景奇; 曾祥晶; 冯海波; 李益亮
湖南中医药大学第二附属医院,湖南 长沙 410005
Author(s):
JIN Chengpeng; SUN Shaoqiu; PENG Zhifei; CHEN Xilong; ZENG Jingqi; ZENG Xiangjing; FENG Haibo; LI Yiliang
The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China
关键词:
桃红四物汤;  血管新生;  受体; Notch;  Delta-like4;  大鼠;  动物实验
Keywords:
Key words Taohong Siwu Tang;  angiogenesis;  receptors; Notch;  Delta-like4;  rats;  animal experimentation
摘要:
目的:观察桃红四物汤对大鼠血管吻合模型吻合区Delta-like4表达及血管新生的影响。方法:将40只3月龄SPF级SD大鼠随机分为空白对照组、假手术组、生理盐水组、桃红四物汤组,每组10只。桃红四物汤组、生理盐水组行尾部动脉血管离断后吻合术; 假手术组仅在相应区域作切口,不进行血管离断吻合; 空白对照组不做任何处理。造模术后桃红四物汤组以20 mL·kg-1剂量的桃红四物汤灌胃,生理盐水组给予等量生理盐水灌胃,每日灌胃2次; 假手术组和空白对照组不给予任何药物干预。分别在药物干预开始后2 d、5 d从各组随机抽取5只大鼠,采用颈椎脱臼法处死后,桃红四物汤组和生理盐水组切取尾部的吻合处血管,假手术组和空白对照组切取尾部相应部位动脉血管。将所取血管组织制成石蜡切片,分成2份,1份采用免疫组化法检测血管吻合区Delta-like4的蛋白表达(光镜下灰度值与Delta-like4的蛋白表达量呈反比); 1份经HE染色后测量吻合区血管内膜面积。结果:①血管吻合区Delta-like4的蛋白表达。药物干预开始后2 d,4组大鼠血管吻合区Delta-like4蛋白表达的灰度值比较,差异无统计学意义(126.27±20.56,111.73±7.99,119.30±16.71,124.53±7.35,F=0.624,P=0.062)。药物干预开始后5 d,4组大鼠血管吻合区Delta-like4蛋白表达的灰度值比较,差异有统计学意义(85.60±5.58,99.07±3.84,103.13±11.61,111.60±4.49,F=7.041,P=0.012); 进一步两两比较,桃红四物汤组血管吻合区Delta-like4蛋白表达的灰度值低于生理盐水组(LSD-t=-2.334,P=0.048)、假手术组(LSD-t=-3.035,P=0.016)、空白对照组(LSD-t=-4.450,P=0.002); 生理盐水组血管吻合区Delta-like4蛋白表达的灰度值与假手术组、空白对照组比较,差异均无统计学意义(LSD-t=-0.703,P=0.502; LSD-t=-2.166,P=0.062); 假手术组血管吻合区Delta-like4蛋白表达的灰度值与空白对照组比较,差异无统计学意义(LSD-t=-1.464,P=0.181)。②吻合区血管内膜面积。药物干预开始后2 d,4组大鼠吻合区血管内膜面积比较,差异无统计学意义[(0.20±0.01)mm2,(0.18±0.02)mm2,(0.16±0.01)mm2,(0.17±0.02)mm2,F=2.625,P=0.122)。药物干预开始后5 d,4组大鼠吻合区血管内膜面积比较,差异有统计学意义[(0.30±0.02)mm2,(0.20±0.01)mm2,(0.21±0.01)mm2,(0.21±0.01)mm2,F=17.063,P=0.000]; 进一步两两比较,桃红四物汤组吻合区血管内膜面积大于生理盐水组(LSD-t=-46.275,P=0.020)、假手术组(LSD-t=-68.142,P=0.000)、空白对照组(LSD-t=-66.154,P=0.000); 生理盐水组吻合区血管内膜面积与假手术组、空白对照组比较,差异均无统计学意义(LSD-t=-2.187,P=0.060; LSD-t=-1.988,P=0.082); 假手术组吻合区血管内膜面积与空白对照组比较,差异无统计学意义(LSD-t=0.199,P=0.847)。结论:桃红四物汤能上调血管吻合大鼠模型吻合区Delta-like4的表达,促进血管新生。
Abstract:
ABSTRACT Objective:To observe the effect of Taohong Siwu Tang(桃红四物汤,THSWT)on Delta-like4 expression and angiogenesis in anastomosed area in vascular anastomose rat models.Methods:Forty 3-month-old SPF-grade SD rats were randomly divided into blank control group,sham-operated group,normal saline(NS)group and THSWT group,10 rats in each group.The surgery of mutilation-anastomose were performed on the caudal artery of rats in THSWT group and NS group,and sham operation were performed on rats in sham-operated group,while the rats in blank control group were not given any surgical intervention.After the end of the modeling operation,the----------------------------------------------- rats in THSWT group were intragastric administrated with THSWT in dosage of 20 ml/kg,and the rats in NS group were intragastric administrated with the same dose of normal saline,twice a day.The rats in sham-operated group and blank control group were not given any drug intervention.At 2 and 5 days after the beginning of drug intervention,5 rats were randomly selected from each group respectively and were executed by using cervical dislocation method.The caudal anastomotic blood vessels of rats were fetched in THSWT group and NS group,and the caudal arterial vessels of rats in corresponding sites were fetched in sham-operated group and blank control group.The acquired vascular tissues were made into paraffin sections and were divided into 2 parts,one of them was applied to measure the Delta-like 4 protein expression by using immunohistochemical method(the gray values were inversely proportional to the delta-like4 protein expression under light microscope),while the other portion was applied to measure the area of endangium in anastomosed area through HE staining.Results:At 2 days after the beginning of the drug intervention,there was no statistical difference in the gray values of Delta-like 4 protein expression in vascular anastomosed area between the 4 groups(126.27+/-20.56,111.73+/-7.99,119.30+/-16.71,124.53+/-7.35,F=0.624,P=0.062).At 5 days after the beginning of the drug intervention,there was statistical difference in the gray values of Delta-like 4 protein expression in vascular anastomosed area between the 4 groups(85.60+/-5.58,99.07+/-3.84,103.13+/-11.61,111.60+/-4.49,F=7.041,P=0.012).Further pairwise comparison showed that the gray values of Delta-like 4 protein expression in vascular anastomosed area were lower in THSWT group compared to NS group(LSD-t=-2.334,P=0.048),sham-operated group(LSD-t=-3.035,P=0.016)and blank control group(LSD-t=-4.450,P=0.002); and there was no statistical difference in the gray values of Delta-like 4 protein expression in vascular anastomosed area between NS group and sham-operated group and between NS group and blank control group(LSD-t=-0.703,P=0.502; LSD-t=-2.166,P=0.062); and there was no statistical difference in the gray values of Delta-like 4 protein expression in vascular anastomosed area between sham-operated group and blank control group(LSD-t=-1.464,P=0.181).At 2 days after the beginning of the drug intervention,there was no statistical difference in the area of endangium in anastomosed area between the 4 groups(0.20+/-0.01,0.18+/-0.02,0.16+/-0.01,0.17+/-0.02 mm2,F=2.625,P=0.122).At 5 days after the beginning of the drug intervention,there was statistical difference in the area of endangium in anastomosed area between the 4 groups(0.30+/-0.02,0.20+/-0.01,0.21+/-0.01,0.21+/-0.01 mm2,F=17.063,P=0.000).Further pairwise comparison showed that the area of endangium in anastomosed area was larger in THSWT group compared to NS group(LSD-t=-46.275,P=0.020),sham-operated group(LSD-t=-68.142,P=0.000)and blank control group(LSD-t=-66.154,P=0.000); and there was no statistical difference in the area of endangium in anastomosed area between NS group and sham-operated group and between NS group and blank control group(LSD-t=-2.187,P=0.060; LSD-t=-1.988,P=0.082); and there was no statistical difference in the area of endangium in anastomosed area between sham-operated group and blank control group(LSD-t=0.199,P=0.847).Conclusion:THSWT can up-regulate Delta-like4 expression and promote angiogenesis in anastomosed area in vascular anastomose rat models.

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相似文献/References:

[1]林念慈,孙绍裘,李益亮,等.桃红四物汤对兔自体皮片移植模型Delta-like 4表达的影响[J].中医正骨,2015,27(11):1.
　LIN Nianci,SUN Shaoqiu,LI Yiliang,et al.Impact of Taohong Siwu Tang(桃红四物汤)on Delta-like 4 expression in the autologous skin graft model of rabbits[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(12):1.
[2]林念慈,孙绍裘,李益亮.Notch信号通路配体对血管新生的影响[J].中医正骨,2015,27(05):70.
[3]刘卓,李益亮,张志,等.Notch4受体对血管新生的影响[J].中医正骨,2016,28(06):56.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81373658); 国家中医药管理局全国名老中医药专家传承工作室建设项目; 湖南省教育厅科学研究项目(15C1058) 通讯作者:李益亮 E-mail:452833225@qq.com
更新日期/Last Update: 2018-05-02