[1]陈旭,余进伟,赵运亮,等.膝关节关节液和血清中骨钙素、基质金属蛋白酶1 及胰岛素样生长因子Ⅰ含量与软骨损伤关系的 初步研究[J].中医正骨,2016,28(02):20-22.
　CHEN Xu,YU Jinwei,ZHAO Yunliang,et al.A pilot study on the relationship between knee cartilage injury and contents of bone gla protein,matrix metalloproteinase 1 and insulin-like growth factorⅠin knee synovial fluid and serum[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2016,28(02):20-22.
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膝关节关节液和血清中骨钙素、基质金属蛋白酶1 及胰岛素样生长因子Ⅰ含量与软骨损伤关系的 初步研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第28卷
期数:
2016年02期
页码:
20-22
栏目:
临床研究
出版日期:
2016-02-20

文章信息/Info

Title:
A pilot study on the relationship between knee cartilage injury and contents of bone gla protein,matrix metalloproteinase 1 and insulin-like growth factorⅠin knee synovial fluid and serum
作者:
陈旭; 余进伟; 赵运亮; 李德荣; 郭甲瑞; 赵意华; 孙冬兰
河南省焦作市第二人民医院,河南 焦作 454001
Author(s):
CHEN Xu; YU Jinwei; ZHAO Yunliang; LI Derong; GUO Jiarui; ZHAO Yihua; SUN Donglan
The Second People's Hospital of Jiaozuo city,Jiaozuo 454001,Henan,China.
关键词:
骨关节炎; 膝;  软骨; 关节;  骨钙素;  基质金属蛋白酶1;  胰岛素样生长因子Ⅰ;  滑液;  血清
Keywords:
osteoarthritis; knee;  cartilage; articular;  osteocalcin;  matrix metalloproteinase 1;  insulin-like growth factorⅠ;  synovial fluid;  serum
摘要:
目的:初步探讨膝关节关节液和血清中骨钙素(bone gla protein,BGP)、基质金属蛋白酶1(matrix metalloproteinase 1,MMP-1)及胰岛素样生长因子Ⅰ(insulin-like growth factorⅠ,IGF-Ⅰ)含量与软骨损伤的关系。方法:纳入80例患者,其中 因膝骨关节炎(knee osteoarthritis,KOA)接受膝关节置换或膝关节镜治疗,直视或镜下确认关节软骨损伤的患者40例(KOA组); 因膝关节游离体或陈旧性交叉韧带损伤或单纯半月板损伤行膝关节镜手术,经关节镜检查关节软骨无明显损伤的患者40例(对照 组)。采用ELISA法测定患者关节液和血清中BGP、MMP-1及IGF-Ⅰ的含量,并进行组间对比。结果:KOA组患者膝关节液和血清中 的BGP、MMP-1及IGF-Ⅰ含量均高于对照组[BGP:(17.91±2.51)μg·L-1,(13.43±1.20)μg·L- 1,t=19.735,P=0.000;(7.04±2.03)μg·L-1,(6.08±1.96)μg·L- 1,t=9.387,P=0.000; MMP-1:(13.79±2.51)μg·L-1,(12.96±2.39)μg·L- 1,t=10.979,P=0.000;(8.01±2.01)μg·L-1,(6.79±1.57)μg·L- 1,t=17.403,P=0.000; IGF-Ⅰ:(15.63±3.51)μg·L-1,(11.63±2.87)μg·L- 1,t=40.970,P=0.000;(6.09±2.39)μg·L-1,(4.30±2.01)μg·L- 1,t=20.340,P=0.000]。结论:测定膝关节关节液和血清中的BGP、MMP-1、IGF-Ⅰ含量,可作为诊断膝关节软骨损伤的辅 助方法之一。
Abstract:
Objective:To explore the relationship between knee cartilage injury and contents of bone gla protein (BGP),matrix metalloproteinase 1(MMP-1)and insulin-like growth factorⅠ(IGF-Ⅰ)in knee synovial fluid and serum.Methods:Eighty patients were enrolled in the study,40 of which were treated with knee replacement or arthroscopy for knee osteoarthritis(KOA)and the articular cartilage injuries were confirmed in them(KOA group),while other patients were treated with knee arthroscopic surgery for knee liber bodies or old cruciate ligament injury or simple meniscus injury and no obvious knee cartilage injuries were found in them(control group).The contents of BGP,MMP-1 and IGF-Ⅰin knee synovial fluid and serum were measured by using ELISA and compared between the 2 groups.Results:The contents of BGP,MMP-1 and IGF-Ⅰin knee synovial fluid and serum were higher in KOA group compared to control group(BGP:17.91+/-2.51 vs 13.43+/-1.20 μg/l,t=19.735,P=0.000; 7.04+/-2.03 vs 6.08+/-1.96 μg/l,t=9.387,P=0.000; MMP-1:13.79+/-2.51 vs 12.96+/-2.39 μg/l,t=10.979,P=0.000; 8.01+/-2.01 vs 6.79+/-1.57 μg/l,t=17.403,P=0.000; IGF-Ⅰ:15.63+/-3.51 vs 11.63+/-2.87 μg/l,t=40.970,P=0.000; 6.09+/-2.39 vs 4.30+/-2.01 μg/l,t=20.340,P=0.000).Conclusion:Quantitative determination of contents of BGP,MMP-1 and IGF-Ⅰin knee synovial fluid and serum can be used as one of the assisted methods for diagnosis of knee cartilage injuries.

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更新日期/Last Update: 2016-04-30