[1]李长明,谢尚举,全仁夫,等.电针秩边穴与水道穴对大鼠急性脊髓损伤后 运动和排尿功能的影响[J].中医正骨,2014,26(12):3-6.
　Li Changming*,Xie Shangju,Quan Renfu,et al.Effect of electroacupuncture Zhibian(BL54)and Shuidao(ST28)on motor function and urination function in rats with acute spinal cord injuries[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(12):3-6.
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电针秩边穴与水道穴对大鼠急性脊髓损伤后 运动和排尿功能的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年12期
页码:
3-6
栏目:
基础研究
出版日期:
2014-12-30

文章信息/Info

Title:
Effect of electroacupuncture Zhibian(BL54)and Shuidao(ST28)on motor function and urination function in rats with acute spinal cord injuries
作者:
李长明1; 谢尚举1; 全仁夫2; 杨宗保3
1.浙江中医药大学,浙江 杭州 310053;
2.浙江省杭州市萧山区中医院, 浙江 杭州 311201;
3.厦门大学医学院,福建 厦门 361000
Author(s):
Li Changming*; Xie Shangju; Quan Renfu; Yang Zongbao.*
Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China
关键词:
脊髓损伤 电针 穴; 秩边 穴; 水道 运动功能 膀胱容量 剩余尿量 大鼠 动物实验
Keywords:
Spinal cord injuries;  Electroacupuncture;  POINT BL54(ZHIBIAN);  POINT ST28(SHUIDAO);  Motor function;  Bladder capacity;  Residual urine volume;  Rats;  Animal experimentation
摘要:
目的:观察电针秩边穴与水道穴对大鼠急性脊髓损伤后运动和排尿功能的影响。方法:将48只SD大鼠随机分为电针组和对 照组,每组24只。采用改良Allen's重物打击法将所有大鼠制成急性脊髓损伤模型。造模后24 h,电针组大鼠在秩边穴和水道穴进 行电针治疗,对照组大鼠在秩边穴和水道穴旁开0.5 cm处进行电针治疗。每天治疗1次,每次15 min。分别于实验干预1 d、3 d、 7 d和14 d后采用BBB评分法评定2组大鼠的运动功能,同时于每次运动功能测试完成后,测定大鼠的膀胱容量和剩余尿量。结果: ①运动功能。干预后不同时间BBB评分的差异有统计学意义,即存在时间效应[(0.50±0.53)分,(2.90±1.10)分, (7.50±1.27)分,(11.50±0.71)分;(0.60±0.52)分,(2.70±0.95)分,(4.60±0.70)分, (8.60±0.97)分; F=625.000,P=0.000]。2组大鼠BBB评分的组间差异总体上有统计学意义,即存在分组效应 (F=29.866,P=0.000); 干预1 d、3 d后,2组大鼠的BBB评分比较,差异均无统计学意义(t=0.429,P=0.678; t=0.435,P=0.674); 干预7 d、14 d后,电针组大鼠的BBB评分均大于对照组(t=6.328,P=0.000; t=9.222,P=0.000)。时间因素和分组因素之间存在交 互效应(F=24.939,P=0.000)。②排尿功能。干预后不同时间膀胱容量的差异有统计学意义,即存在时间效应[(1.10±0.05) mL,(1.37±0.05)mL,(1.89±0.09)mL,(2.31±0.19)mL;(1.10±0.06)mL,(1.36±0.05)mL, (1.67±0.08)mL,(1.88±0.08)mL; F=193.885,P=0.000]。2组大鼠膀胱容量的组间差异总体上有统计学意义,即存在分 组效应(F=65.970,P=0.000); 干预1 d、3 d后,2组大鼠的膀胱容量比较,差异均无统计学意义(t=0.177,P=0.868; t=0.366,P=0.733); 干预7 d、14 d后,电针组大鼠的膀胱容量均大于对照组(t=4.186,P=0.014; t=4.647,P=0.009)。时间因素 和分组因素之间存在交互效应(F=10.283,P=0.000)。干预后不同时间剩余尿量的差异有统计学意义,即存在时间效应 [(2.00±0.20)mL,(1.88±0.15)mL,(1.49±0.07)mL,(0.98±0.11)mL;(1.99±0.19)mL, (1.89±0.17)mL,(1.73±0.12)mL,(1.29±0.07)mL; F=131.825,P=0.000]。2组大鼠剩余尿量的组间差异总体上有 统计学意义,即存在分组效应(F=3.787,P=0.038); 干预1 d、3 d后,2组大鼠的剩余尿量比较,差异均无统计学意义 (t=0.016,P=0.988; t=0.039,P=0.971); 干预7 d、14 d后,电针组大鼠的剩余尿量均小于对照组(t=3.791,P=0.019; t=5.334,P=0.006)。时间因素和分组因素之间存在交互效应(F=5.646,P=0.028)。结论:电针秩边穴与水道穴能改善大鼠急性脊 髓损伤后的运动和排尿功能。
Abstract:
Objective:To observe the effect of electroacupuncture Zhibian(BL54)and Shuidao(ST28)on motor function and urination function in rats with acute spinal cord injuries.Methods:Forty-eight SD rats were randomly divided into electroacupuncture group and control group,24 cases in each group.The acute spinal cord injuries models were built by modified Allen's method in all rats.The rats in the electroacupuncture group were treated with electroacupuncture at Zhibian(BL54)and Shuidao(ST28),while the others in the control group were treated with electroacupuncture at points 0.5 cm apart from Zhibian(BL54)and Shuidao(ST28)at 24 hours after the modeling,once a day for 15 minutes at a time.The motor function were evaluated by using BBB score at 1st,3rd,7th and 14th days after experimental intervention.Meanwhile,the bladder capacity and residual urine volume of the rats were measured.Results:There was statistical difference in BBB scores between different time points after intervention,in other words,there was time effect(0.50+/-0.53,2.90+/- 1.10,7.50+/ 1.27,11.50+/-0.71 points; 0.60+/-0.52,2.70+/-0.95,4.60+/-0.70,8.60+/-0.97 points; F=625.000,P=0.000).In general,there was statistical difference in BBB scores between the 2 groups,in other words,there was grouping effect(F=29.866,P=0.000).There was no statistical difference in BBB scores between the 2 groups at 1 day and 3 days after intervention(t=0.429,P=0.678; t=0.435,P=0.674),and the BBB scores of the electroacupuncture group were greater than those of control group at 7 and 14 days after intervention(t=6.328,P=0.000; t=9.222,P=0.000).There was interaction between time factor and grouping factor(F=24.939,P=0.000).There was statistical difference in bladder capacity between different time points after intervention,in other words,there was time effect(1.10+/-0.05,1.37+/-0.05,1.89+/-0.09,2.31+/-0.19 mL; 1.10+/-0.06,1.36+/- 0.05,1.67+/-0.08,1.88+/-0.08 mL; F=193.885,P=0.000).In general,there was statistical difference in bladder capacity between the 2 groups,in other words,there was grouping effect(F=65.970,P=0.000).There was no statistical difference in bladder capacity between the 2 groups at 1 day and 3 days after intervention (t=0.177,P=0.868; t=0.366,P=0.733),and the bladder capacity of the electroacupuncture group was larger than that of control group at 7 and 14 days after intervention(t=4.186,P=0.014; t=4.647,P=0.009).There was interaction between time factor and grouping factor(F=10.283,P=0.000).There was statistical difference in residual urine volume between different time points after intervention,in other words,there was time effect (2.00+/-0.20,1.88+/-0.15,1.49+/-0.07,0.98+/-0.11 mL; 1.99+/-0.19,1.89+/-0.17,1.73+/-0.12,1.29+/-0.07 mL; F=131.825,P=0.000).In general,there was statistical difference in residual urine volume between the 2 groups,in other words,there was grouping effect(F=3.787,P=0.038).There was no statistical difference in residual urine volume between the 2 groups at 1 day and 3 days after intervention(t=0.016,P=0.988; t=0.039,P=0.971),and the residual urine volume of the electroacupuncture group was smaller than that of control group at 7 and 14 days after intervention(t=3.791,P=0.019; t=5.334,P=0.006).There was interaction between time factor and grouping factor(F=5.646,P=0.028).Conclusion:The motor function and urination function of rats with acute spinal cord injuries can be improved by electroacupuncture Zhibian(BL54)and Shuidao(ST28).

参考文献/References:

[1] 杨拯,何彦芳,陈建敏,等.电针在脊髓损伤功能康复中的研究[J].时珍国医国药,2011,22(1):249-250.
[2] 张芸.针刺取穴与脊髓损伤恢复的关系探讨[J].中国中医骨伤科杂志,2002,10(6):58-59.
[3] Khan T,Havey RM,Sayers ST,et al.Animal models of spinal cord contusion injuries[J].Lab Anim Sci,1999,49 (2):161-172.
[4] Scheff SW,Saucier DA,Cain ME.A statistical method for analyzing rating scale data:the BBB locomotor score [J].J Neurotrauma,2002,19(10):1251-1260.
[5] 张丽,林海燕,屠文展,等.电针对慢性期脊髓损伤大鼠功能恢复及神经营养因子表达的影响[J].中国康复医学杂志,2014,29 (4):311-315.
[6] 张军,向剑锋,马笃军,等.电针刺激治疗脊髓损伤后神经源性膀胱临床观察[J].中国中医急症,2013,22(5):813-814.
[7] 邱茂良.针灸学[M].上海:上海科技出版社,1975:83.
[8] 杨兆钢,冀来喜.芒针深刺秩边穴的临床应用研究[J].中国针灸,1997,02(2):72-74.
[9] 刘宝贵,冀来喜,郝重耀,等.秩边透水道针法治疗慢性前列腺炎的解剖学基础[J].中国针灸,2001,21(2):91-93.
[10] 臧大维,刘娟,Cheema S.脊髓损伤后神经营养因子及干细胞治疗对轴突再生的影响:国外基础和临床研究新进展[J].中国组 织工程研究与临床康复,2007,11(14):2787-2791, 2796.
[11] Lee Y,Lee S,Lee SR,et al.Beneficial effects of melatonin combined with exercise on endogenous neural stem/progenitor cells proliferation after spinal cord injury[J].Int J Mol Sci,2014,15(2):2207-2222.
[12] Chen J,Qi JG,Zhang W,et al.Electro-acupuncture induced NGF,BDNF and NT-3 expression in spared L6 dorsal root ganglion in cats subjected to removal of adjacent ganglia[J].Neurosci Res,2007,59(4):399-405.

备注/Memo

备注/Memo:
基金项目:浙江省中医药科研计划项目(2008CB067)
通讯作者:全仁夫 E-mail:quanrenf@263.net
更新日期/Last Update: 2014-12-30