[1]伍海昭,朱敏,詹红生,等.骨碎补总黄酮对去卵巢大鼠骨组织Ⅰ型胶原表达及骨代谢的影响[J].中医正骨,2014,26(04):11-14.
　Wu Haizhao*,Zhu Min,Zhan Hongsheng,et al.Effect of drynaria fortunei total flavonoids on collagen typeⅠexpression in bone tissue and bone metabolism in the ovariectomized rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(04):11-14.
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骨碎补总黄酮对去卵巢大鼠骨组织Ⅰ型胶原表达及骨代谢的 影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年04期
页码:
11-14
栏目:
基础研究
出版日期:
2014-04-30

文章信息/Info

Title:
Effect of drynaria fortunei total flavonoids on collagen typeⅠexpression in bone tissue and bone metabolism in the ovariectomized rats
作者:
伍海昭1; 朱敏1; 詹红生2; 陈海啸1
1.浙江省台州医院,浙江 临海 317000; 2.上海中医药大学附属曙光医院,上海 201203
Author(s):
Wu Haizhao*; Zhu Min; Zhan Hongsheng; Chen Haixiao.*
The Taizhou hospital of Zhejiang province,Linhai 317000,Zhejiang,China
关键词:
骨碎补 骨质疏松; 绝经后 胶原Ⅰ型 骨密度 大鼠 动物实验
Keywords:
Drynaria fortunei;  Osteoporosis; postmenopausal;  Collagen typeⅠ;  Bone density;  Rats;  Animal experimentation
摘要:
目的:观察骨碎补总黄酮对去卵巢大鼠骨组织Ⅰ型胶原表达及骨代谢的影响。方法:雌性SD大鼠40只,月龄10个月,随机分为实验组、空白对照组、模型组和阳性对照组,每组10只。除空白对照组外,其余各组均切除双侧卵巢制作骨质疏松模型。造模后第8天开始药物干预,实验组予以骨碎补总黄酮浓缩液2 mL灌胃,阳性对照组予以倍美力混悬剂2 mL灌胃; 空白对照组和模型组予以生理盐水2 mL灌胃; 均每日1次。药物干预6个月后,比较各组大鼠骨密度、尿脱氧吡啶啉/尿肌酐比值、Ⅰ型胶原氨基末端肽/尿肌酐比值及血清Ⅰ型前胶原氨基端前肽含量; 并检测各组大鼠骨组织中Ⅰ型胶原mRNA的表达。结果:药物干预6个月后,各组大鼠间骨密度值比较,差异有统计学意义[(0.287±0.017)g·cm-2,(0.362±0.031)g·cm-2,(0.207±0.008)g·cm-2,(0.291±0.015)g·cm-2; F=104.317,P=0.000)]; 实验组低于空白对照组(P=0.000),高于模型组(P=0.000),但与阳性对照组比较,差异无统计学意义(P=0.584); 空白对照组高于模型组和阳性对照组(P=0.000,P=0.000); 模型组低于阳性对照组(P=0.000)。各组间尿脱氧吡啶啉/尿肌酐比值(3.381±0.202,2.462±0.310,4.526±0.243,3.232±0.191)、Ⅰ型胶原氨基末端肽/尿肌酐比值(10.901±1.265,8.638±1.036,13.392±1.959,10.516±1.362)及血清Ⅰ型前胶原氨基端前肽含量[(232.081±28.452)μg·mL-1,(184.443±19.250)μg·mL-1,(283.262±33.628)μg·mL-1,(238.861±26.685)μg·mL-1]比较,差异均有统计学意义(F=124.841,P=0.000; F=18.277,P=0.000; F=21.648,P=0.000); 实验组高于空白对照组,差异均有统计学意义(P=0.000,P=0.000,P=0.000); 实验组低于模型组,差异有统计学意义(P=0.000,P=0.003,P=0.002); 实验组与阳性对照组比较,差异均无统计学意义(P=0.107,P=0.521,P=0.589); 空白对照组低于模型组和阳性对照组,差异有统计学意义(P=0.000,P=0.000,P=0.000; P=0.000,P=0.003,P=0.000); 模型组高于阳性对照组,差异均有统计学意义(P=0.000,P=0.001,P=0.004)。空白对照组大鼠骨组织Ⅰ型胶原mRNA表达最强; 模型组大鼠骨组织Ⅰ型胶原mRNA表达呈阴性或低表达; 实验组大鼠骨组织Ⅰ型胶原mRNA表达水平与阳性对照组接近,较模型组强。结论:骨碎补总黄酮能增强去卵巢大鼠骨组织Ⅰ型胶原的表达,降低骨转换率,增加骨密度; 这可能是骨碎补总黄酮防治骨质疏松症的机制之一。
Abstract:
Objective:To observe the effect of drynaria fortunei total flavonoids on collagen typeⅠ(COL-Ⅰ)expression in bone tissue and bone metabolism in the ovariectomized rats.Methods:Forty ten-month-old female SD rats were randomly divided into experimental group,blank control group,model group and positive control group,10 rats in each group.The osteoporosis rat models were built by bilateral ovariectomy in experimental group,model group and positive control group.The drug intervention were carried out from the 8th day after modeling.The rats in the experimental group were intragastric administrated with concentrated solution of drynaria fortunei total flavonoids(2 mL qd),the rats in the positive control group were intragastric administrated with Premarin suspensions(2 mL qd),while the others in the blank control group and model group were intragastric administrated with normal saline(2 mL qd).After 6 months of drug intervention,bone density,urine content ratio of deoxy pyridinoline(DPD)to creatinine(Cre),urine content ratio of N-telopeptide of typeⅠcollagen(NTx)to creatinine(Cre)and serum content of amino-terminal propeptide of type Ⅰ procollagen(PINP)were detected and compared between the 4 groups.Meanwhile,the COL-ⅠmRNA expression in bone tissues were detected.Results:After drug intervening for 6 months,there were statistical differences in the bone density between the 4 groups(0.287+/-0.017,0.362+/-0.031,0.207+/-0.008,0.291+/-0.015 g/cm(2); group was lower than that of the blank control group(P=0.000)and higher than that of model group(P=0.000),while there was no statistical difference in the bone density value between the experimental group and positive control group(P=0.584).The bone density value of the blank control group was higher than that of model group and positive control group(P=0.000,P=0.000),while the bone density value of the model group was lower than that of positive control group(P=0.000).There were statistical differences in the urine content ratio of DPD to Cre(3.381+/-0.202,2.462+/-0.310,4.526+/-0.243,3.232+/-0.191),the urine content ratio of NTx to Cre(10.901+/-1.265,8.638+/-1.036,13.392+/-1.959,10.516+/-1.362)and the serum content of PINP(232.081+/-28.452,184.443+/-19.250,283.262+/-33.628,238.861+/-26.685 μg/mL)between the 4 groups(F=124.841,P=0.000; F=18.277,P=0.000; F=21.648,P=0.000).The values of these bone metabolism indexes were higher in the experimental group compared with the blank control group(P=0.000,P=0.000,P=0.000)and were lower in the experimental group compared with the model group(P=0.000,P=0.003,P=0.002).There were no statistical differences in the values between the experimental group and the positive control group(P=0.107,P=0.521,P=0.589).The values of the blank control group were lower than those of the model group and the positive control group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.003,P=0.000).The values of the model group were higher than those of the positive control group(P=0.000,P=0.001,P=0.004).Strong-positive expression of COL-ⅠmRNA in bone tissues was found in the blank control group,while negative or low expression of COL-ⅠmRNA was found in the model group.The experimental group was similar to the positive control group and surpassed the model group in the expression of COL-ⅠmRNA in bone tissues.Conclusion:The drynaria fortunei total flavonoids can enhance the COL-Ⅰexpression in bone tissues of ovariectomized rats and reduce the bone turnover rate and increase the bone density,which may be one of the mechanisms for drynaria fortunei total flavonoids in the prevention of osteoporosis.

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更新日期/Last Update: 1900-01-01