[1]王文波,董建文,曹宁,等.碳-碳复合材料羟基磷灰石涂层腰椎融合器的生物相容性研究[J].中医正骨,2014,26(04):7-10.
　Wang Wenbo*,Dong Jianwen,Cao Ning,et al.A biocompatibility study on carbon-carbon composite lumbar cage coated with hydroxyapatites[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(04):7-10.
点击复制

碳-碳复合材料羟基磷灰石涂层腰椎融合器的生物相容性研究()
分享到：

《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年04期
页码:
7-10
栏目:
基础研究
出版日期:
2014-04-30

文章信息/Info

Title:
A biocompatibility study on carbon-carbon composite lumbar cage coated with hydroxyapatites
作者:
王文波1; 董建文1; 曹宁2; 杨振国1; 刘文斌1; 李木森3; 李春娥1
1.山东中医药大学,山东 济南 250355; 2.中国石油大学(华东),山东 青岛 266580; 3.山东大学,山东 济南 250061
Author(s):
Wang Wenbo*; Dong Jianwen; Cao Ning; Yang Zhenguo; Liu Wenbin; Li Musen; Li Chune.*
Shandong University of Traditional Chinese Medicine,Jinan 250355,Shangdong,China
关键词:
腰椎 碳-碳复合材料 羟基磷灰石类 腰椎融合器 动物实验
Keywords:
Lumbar vertebrae;  Carbon-carbon composites;  Hydroxyapatites;  Lumbar cage;  Animal experimentation
摘要:
目的:探讨碳-碳复合材料羟基磷灰石涂层腰椎融合器的生物相容性和融合效果。方法:在8只成年雄性杂交波尔山羊L4～5或L5～6椎间隙模拟椎间融合手术,在相应椎间隙置入自制的碳-碳复合材料羟基磷灰石涂层腰椎融合器,并以克氏针后路固定。观察记录山羊术后生命体征及双下肢活动情况; 分别于术前和处死动物前进行腰椎X线检查,观察腰椎融合器的位置、融合器与上下位椎体的融合情况及上下位椎体的椎间隙; 分别于术后60 d和90 d各处死4只山羊,取融合的2个椎体,分别以肉眼、光镜和电镜进行组织学观察。结果:X线检查显示,术前、术后60 d和术后90 d腰椎融合器上下位椎体间距分别为(2.32±0.31)mm、(2.41±0.43)mm和(2.40±0.44)mm; 术后60 d和术后90 d,与融合器相邻的终板位置有成骨现象和融合现象。组织学观察显示,术后60 d时融合器植骨区和融合器周围有骨小梁生成; 术后90 d时骨小梁排列逐渐规则,融合器周围有一层纤维结缔组织生成,结缔组织逐渐变薄,融合器中间有骨组织和纤维组织长入。结论:碳-碳复合材料羟基磷灰石涂层腰椎融合器具有较好的生物相容性和稳定性,有利于植骨融合,可作为椎间融合的内固定器材。
Abstract:
Objective:To explore the biocompatibility of carbon-carbon composite lumbar cage coated with hydroxyapatites(HA)and the effectiveness of fusion.Methods:The simulated intervertebral fusion surgery were performed in L4/L5 or L5/L6 intervertebral space in 8 adult male hybrid Boer goats.The self-made carbon-carbon composite lumbar cage coated with HA were placed in the intervertebral space and fixed with Kirschner-wire in posterior access.Vital signs and both lower extremities activities of the Boer goats were observed and recorded after the surgery.Radiography were performed in the lumbar vertebraes before the surgery and before the goats were sacrificed respectively.The location of lumbar cage,fusion of lumbar cage and vertebral bodies and the intervertebral space were observed.Four goats were sacrificed at 60th days and 90th days after the surgery respectively,and the two confertus vertebral bodies were fetched,and the histological observation was carried out under naked eye,optical microscope and electron microscope.Results:The X-ray examination showed that the height of intervertebral space where the lumbar cage were placed were 2.32+/-0.31,2.41+/-0.43 and 2.40+/-0.44 mm respectively before the surgery,60 days and 90 days after the surgery.Bone formation and fusion were found in the endplate adjacent to lumbar cage 60 days and 90 days after the surgery.The histological observation showed that the trabeculae of bone could be found in the bone graft area of lumbar cage and around the lumbar cage 60 days after the surgery.The trabecular bone arranged regularly at the 90 days after the surgery,a layer of fibrous connective tissue were found around the cage and it grow thin gradually,and the bone tissue and fibrous tissue grew into the cage.Conclusion:The carbon-carbon composite lumbar cage coated with HA has good biocompatibility and stability,and it is helpful to bone graft fusion,so it can be used as the internal fixation apparatus for intervertebral fusion.

参考文献/References:

[1] Virgil'ev YS,Kalyagina IP.Carbon-carbon composite materials[J].Inorganic Materials,2004,40(Suppl 1):33-49.
[2] 李翠云,李辅安.碳/碳复合材料的应用研究进展[J].化工新型材料,2006,34(3):18-20.
[3] 曹宁.等离子喷涂法制备碳/碳复合材料表面HA涂层技术的应用研究[D].济南:山东大学,2007.
[4] Delfosse C,Monchau F,Lefevre A,et al.Biological characterization of experimental carbon samples[J].Bull Group Int Rech Sci Stomatol Odontol, 2002,44(3):73-82.
[5] 俞扭庭,张兴栋.生物医用材料[M].天津:天津大学出版社,2000:138.

备注/Memo

备注/Memo:
基金项目:山东省医药卫生发展计划项目(2007HZ093),山东省济南市科技发展计划项目(201102063) 通讯作者:董建文 E-mail:dongjianwen2006@sina.com
更新日期/Last Update: 1900-01-01