[1]谢义松,张紫铭,吴官保,等.虎潜丸对去卵巢大鼠骨质疏松模型骨密度及 转化生长因子β1表达的影响[J].中医正骨,2013,25(12):11-14.
　Xie Yisong*,Zhang Ziming,Wu Guanbao,et al.Impact of HUQIAN PILL on bone density and transforming growth factor beta1 expression in the ovariectomized osteoporosis rat model[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2013,25(12):11-14.
点击复制

虎潜丸对去卵巢大鼠骨质疏松模型骨密度及 转化生长因子β1表达的影响()
分享到：

《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第25卷
期数:
2013年12期
页码:
11-14
栏目:
骨质疏松症
出版日期:
2013-12-30

文章信息/Info

Title:
Impact of HUQIAN PILL on bone density and transforming growth factor beta1 expression in the ovariectomized osteoporosis rat model
作者:
谢义松1; 张紫铭2; 吴官保3; 朱建华2; 尤冬春2
1.湖南中医药大学第二附属医院,湖南 长沙 410005;
2.湖南中医药大学, 湖南 长沙 410007;
3.湖南省中医药研究院附属医院,湖南 长沙 410006
Author(s):
Xie Yisong*; Zhang Ziming; Wu Guanbao; Zhu Jianhua; You Dongchun.*
The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China
关键词:
骨质疏松 骨密度 转化生长因子β1 动物实验 大鼠 虎潜丸
Keywords:
Osteoporosis;  Bone density;  Transforming growth factor beta1;  Animal experimentation;  Rats;  HUQIAN PILL
摘要:
目的:探讨虎潜丸治疗骨质疏松症的作用机制。方法:雌性SD大鼠40只,体质量180～220 g,中位数200 g,随机分为虎潜丸组、雌激素组、模型组、假手术组,每组10只。虎潜丸组、雌激素组、模型组切除双侧卵巢造模。7 d后,虎潜丸组予以虎潜丸水煎液灌胃,雌激素组予以尼尔雌醇混悬液灌胃,模型组、假手术组予以蒸馏水灌胃; 每100 g体质量0.875 mL,每日灌胃1次,连续干预12周。比较各组大鼠椎骨骨密度及椎骨、肾组织中转化生长因子β1的表达。结果:虎潜丸组和雌激素组各有1只大鼠死亡,共纳入研究38只。干预12周后,各组大鼠椎骨骨密度值比较,差异有统计学意义[(0.210±0.028)g·cm-2,(0.205±0.030)g·cm-2,(0.171±0.036)g·cm-2,(0.205±0.034)g·cm-2; F=7.561,P=0.001]; 虎潜丸组、雌激素组、假手术组均高于模型组(P=0.002,P=0.002,P=0.013); 虎潜丸组与雌激素组、假手术组相比,差异均无统计学意义(P=0.459,P=0.224); 雌激素组与假手术组相比,差异无统计学意义(P=0.231)。各组大鼠椎骨转化生长因子β1灰度值比较,差异无统计学意义(169.060±5.545,167.420±6.238,162.400±3.882,168.130±7.899; F=2.176,P=0.109)。各组大鼠肾组织转化生长因子β1光密度值比较,差异有统计学意义(172.600±5.019,158.200±8.038,155.130±4.542,157.570±5.524; F=15.568,P=0.000); 虎潜丸组高于雌激素组、模型组和假手术组(P=0.000; P=0.000; P=0.000); 雌激素组与模型组、假手术组比较,差异均无统计学意义(P=0.478,P=0.490); 假手术组高于模型组(P=0.038)。结论:虎潜丸可增加大鼠腰椎椎骨骨密度,上调肾组织中转化生长因子β1的表达,可能是其治疗骨质疏松症的机制之一。
Abstract:
Objective:To explore the mechanisms of action of HUQIAN PILL in the treatment of osteoporosis.Methods:Forty female SD rats,ranged in weight from 180 to 220 g(Median=200 g),were randomly divided into HUQIAN PILL group,estrogen group,model group and sham-operation group,10 rats in each group.The rats models of osteoporosis were created by ovariectomy in HUQIAN PILL group,estrogen group and model group.Seven days after operation,rats were intragastric administrated with HUQIAN PILL decoction,nilestriol suspension and distilled water(0.875 mL/100 g)respectively,one time daily for 12 consecutive weeks.The bone density of vertebrae and transforming growth factor beta1(TGFβ1)expression in vertebra and nephridial tissue were detected and compared among the 4 groups.Results:One rat died in HUQIAN PILL group and one rat died in estrogen group,so 38 rats were included in the study.After 12 weeks of drug intervention,there were statistical differences in the bone density values of rat vertebra among the 4 groups(0.210+/-0.028,0.205+/-0.030,0.171+/-0.036,0.205+/-0.034 g/cm(2),F=7.561,P=0.001).The bone density values of HUQIAN PILL group,estrogen group and sham-operation group were all higher than that of model group(P=0.002,P=0.002,P=0.013).The HUQIAN PILL group was similar to estrogen group and sham-operation group in the bone density values(P=0.459,P=0.224),and there were no statistical differences between estrogen group and sham-operation group(P=0.231).There were no statistical differences in the gray values of TGF-β1 in vertebra among the 4 groups(169.060+/-5.545,167.420+/-6.238,162.400+/-3.882,168.130+/-7.899,F=2.176,P=0.109).There were statistical differences in the optical density(OD)values of TGF-β1 in nephridial tissue among the 4 groups(172.600+/-5.019,158.200+/-8.038,155.130+/-4.542,157.570+/-5.524,F=15.568,P=0.000).The OD value of TGF-β1 of HUQIAN PILL group was higher than that of estrogen group,model group and sham-operation group respectively(P=0.000,P=0.000,P=0.000).Estrogen group was similar to model group and sham-operation group(P=0.478,P=0.490)in the OD value of TGF-β1,and the sham-operation group surpassed the model group(P=0.038).Conclusion:The bone density of lumbar vertebra can be increased and the expression of TGF-β1 in nephridial tissue can be up-regulated in rat model by using HUQIAN PILL,and it may be one of the mechanisms of action for HUQIAN PILL in the treatment of osteoporosis.

参考文献/References:

[1] 胡永红,李丽春,梁燕,等.中医药治疗肾阳虚型骨质疏松症研究进展[J].河北中医药学报,2012,27(4):42-43.
[2] 刘静仪,林如平.虎潜丸加减治疗骨质疏松症30例疗效观察[J].成都医药,2004,30(3):134-135.
[3] 田茂友,李洪祥.去卵巢大鼠骨质疏松模型研究[J].现代预防医学,2007,34(12):2239-2241.
[4] 邓洋洋,王思程,郑洪新,等.补肾中药对去卵巢骨质疏松症模型大鼠骨密度、子宫指数影响的实验研究[J].辽宁中医杂志,2012,39(9):1867-1869.
[5] 李洪成,郭素华,林如平,等.补肾药虎潜丸对肾虚患者骨密度的影响[J].中国中西医结合杂志,1997,17(11):669-670.
[6] 任艳玲,郑洪新,杜松.补肾健脾药物血清对大鼠成骨细胞Smad2mRNA表达影响的研究[J].中医药学刊,2005,23(4):618.

备注/Memo

备注/Memo:
2013-08-23收稿 2013-10-13修回
基金项目:湖南省科技厅科研基金项目(2011SK3106)
更新日期/Last Update: 2013-12-30