[1]戴七一,文宗振,辛晓东,等.逍遥散对雌性兔膝关节软骨细胞超微结构的影响[J].中医正骨,2012,24(09):8-10.
　DAI Qi-yi*,WEN Zong-zhen,XIN Xiao-dong,et al.Study on the effect of XIAOYAO POWDERS on ultrastructure of knee-joint chondrocytes in female rabbits[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(09):8-10.
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逍遥散对雌性兔膝关节软骨细胞超微结构的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期数:
2012年09期
页码:
8-10
栏目:
基础研究
出版日期:
2012-09-30

文章信息/Info

Title:
Study on the effect of XIAOYAO POWDERS on ultrastructure of knee-joint chondrocytes in female rabbits
作者:
戴七一1; 文宗振2; 辛晓东1; 覃学流1; 刘靖3; 腾居赞1;  朱江龙1; 韩杰1; 欧国建1; 袁经阳1; 郝高庭1
1.广西中医学院附属瑞康医院,广西 南宁 530011;
2.广西大学,广西 南宁 530004;
3.广西中医学院,广西 南宁 530001
Author(s):
DAI Qi-yi*; WEN Zong-zhen; XIN Xiao-dong; QIN Xue-liu; LIU Jing; TENG Ju-zan; ZHU Jiang-long; HAN Jie; OU Guo-jian; YUAN Jing-yang; HAO Gao-ting.*
Ruikang Hospital affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,Guangxi,China
关键词:
骨关节炎; 膝 逍遥散 软骨细胞 兔 动物实验
Keywords:
Osteoarthritis; Knee;  XIAOYAO POWDERS;  Chondrocytes;  Rabbits;  Animal experimentation
摘要:
目的:探讨逍遥散对雌性兔膝关节软骨细胞超微结构的影响。方法:采用改良Huith法对30只雌性新西兰兔右膝关节进行膝骨关节炎造模,造模1周后将30只实验兔随机分为逍遥丸组、玻璃酸钠组及对照组,每组10只。逍遥丸组每天每只给予8丸逍遥丸(相当于原药材3 g),配成30 mL液体灌胃,连用5周; 玻璃酸钠组予以玻璃酸钠注射液0.15 mL·kg-1右膝关节内注射,每周1次,连用5周; 对照组每天予以30 mL生理盐水灌胃,连用5周。造模后12周末处死所有实验兔,取右膝内侧胫骨平台软骨组织切片后透射电镜观察。结果:逍遥丸组细胞核正常,核膜完整,核质均匀,线粒体嵴部分模糊,出现空泡化现象,内质网、高尔基体正常,纤维组织稍紊乱,有凋亡小体存在; 玻璃酸钠组细胞核正常,核膜完整,核质均匀,线粒体空泡化较轻,内质网、高尔基体正常,纤维组织稍紊乱,有凋亡小体存在; 对照组细胞核可见固缩现象,线粒体异常,表现为线粒体嵴模糊、消失,空泡化现象较明显,内质网、高尔基体基本正常,纤维组织紊乱现象较明显,多个标本见凋亡小体。结论:逍遥散对雌性兔膝关节软骨细胞具有保护作用。
Abstract:
Objective:To explore the effect of XIAOYAO POWDERS on the ultrastructure of knee-joint chondrocytes in female rabbits.Methods:Models of knee osteoarthritis(KOA)were built in right knee joints of 30 female New Zealand rabbits by the improved Huith method.One week after modeling,30 experimental rabbits were randomly divided into XIAOYAO POWDERS group,sodium hyaluronate group and control group,10 cases in each group.Every rabbit in the XIAOYAO POWDERS group was intragastric administrated with 30 mL liquid which made from 8 XIAOYAO POWDERS(which dosage was equivalent to that of 3 g original medicine)every day for consecutive 5 weeks.Rabbits in the sodium hyaluronate group were injected with 0.15 mL·kg-1 sodium hyaluronate injection in their right knee joints once a week for consecutive 5 weeks,while the others in the control group were intragastric administrated with 30 mL normal saline every day for consecutive 5 weeks.At the end of 12 weeks after modeling,all the experimental rabbits were executed,and sections from the tibial plateau cartilage tissue inside of their right knees were fetched out to be observed under transmission electron microscope.Results:Such situations were found in XIAOYAO POWDERS group as normal cell nucleus,complete nuclear membrane,uniform nucleoplasm,partially obscuring in mitochondrial crista,vacuolation,normal endoplasmic reticulum and Golgi's apparatus,slightly disorder in fibrous tissue and existence of apoptotic bodies.Such phenomenons were found in sodium hyaluronate group as normal cell nucleus,complete nuclear membrane,uniform nucleoplasm,lesser vacuolation of mitochondria,normal endoplasmic reticulum and Golgi's apparatus,slightly disorder in fibrous tissue and existence of apoptotic bodies.The conditions appeared in a number of specimens of control group as pycnosis of cell nucleus,abnormal mitochondria shown as blurred or disappeared mitochondrial crista,much more obviously vacuolation of mitochondria,essentially normal endoplasmic reticulum and Golgi's apparatus,much more obviously disorder in fibrous tissue and apoptotic bodies.Conclusion:XIAOYAO POWDERS has protective effect on knee-joint chondrocytes in female rabbits.

参考文献/References:

[1] 任海龙,邢国胜,白人骁.雌激素与骨性关节炎[J].中国骨伤,2005,18(12):766-768.
[2] 王泽华.妇产科治疗学[M].北京:人民卫生出版社,2009:734-741.
[3] 刘献祥,李西海,周江涛.改良Hulth造模法复制膝骨性关节炎的实验研究[J].中国中西医结合杂志,2005,25(12):1104-1108.
[4] 施桂英.关节炎概要[M].北京:中国医药科技出版社,2002:327.
[5] Buckwalter JA,Einhorn TA,Simon SR.骨科基础科学[M].陈启明,梁国穗,秦岭,等译.北京:人民卫生出版社,2001.
[6] 戴七一,刘百玲,韦家宁,等.膝骨性关节炎的分型与性别、年龄的关系[J].中医正骨,2007,19(10):12-13.
[7] 代岭辉,杜宁.关节软骨损伤生物学修复的研究进展[J].中国骨伤,2009,22(9):721-724.
[8] Rossouw JE,Anderson GL,Prentice RL,et al.Risks and benefits of estrogen plus progestin in healthy postmenopausal women:principal results From the Women's Health Initiative randomized controlled trial[J].JAMA,2002,288(3):321-333.

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备注/Memo

备注/Memo:
2010-08-21收稿 2012-08-29修回
基金项目:广西区卫生厅重点课题资助项目(重200856)
通讯作者:覃学流 E-mail:Qinxueliu2008@163.com
更新日期/Last Update: 2012-09-30