[1]汤剑斌,圣小平,樊天佑.电针治疗对膝骨关节炎兔膝关节软骨细胞凋亡的影响[J].中医正骨,2012,24(04):12-15.
　TANG Jian-bin*,SHENG Xiao-ping,FAN Tian-you.*.Study on the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(04):12-15.
点击复制

电针治疗对膝骨关节炎兔膝关节软骨细胞凋亡的影响()
分享到：

《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期数:
2012年04期
页码:
12-15
栏目:
基础研究
出版日期:
2012-04-30

文章信息/Info

Title:
Study on the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis
作者:
汤剑斌1; 圣小平2; 樊天佑2
1.上海市浦东新区光明中医医院,上海 201399;
2.上海市中医医院,上海 200071
Author(s):
TANG Jian-bin*; SHENG Xiao-ping; FAN Tian-you.*
Guangming Hospital of Traditional Chinese Medicine of Pudong New Area in Shanghai City,Shanghai 201399,China
关键词:
骨关节炎; 膝 电针 软骨细胞 细胞凋亡
Keywords:
Osteoarthritis; knee;  Electroacupuncture;  Chondrocytes;  Apoptosis
摘要:
目的:观察电针治疗对膝骨关节炎兔膝关节软骨细胞凋亡的影响,探讨电针治疗膝骨关节炎的作用机制。方法:将30只健康新西兰大白兔随机分为正常对照组、模型对照组和电针治疗组,每组10只。采用Hulth法对模型对照组及电针治疗组实验兔左膝关节进行造模,造模后第5周开始对电针治疗组实验兔进行电针治疗,正常对照组、模型对照组同期正常饲养,不作任何治疗。治疗4周后同时处死3组实验兔,取左膝关节股骨端制成切片,观察各组实验兔软骨组织形态及软骨细胞凋亡情况。结果:①软骨组织形态。正常对照组软骨表层光滑、平整,4层结构清晰,软骨细胞分布均匀、无簇聚,潮线完整; 模型对照组关节软骨厚度减小,表面毛糙,可见明显纤维化、裂隙、软骨细胞簇聚,潮线断裂; 电针治疗组关节软骨厚度减小,表面不光滑,少见纤维化、裂隙、软骨细胞簇聚,潮线断裂。②软骨细胞凋亡情况。3组实验兔软骨细胞凋亡率比较,差异有统计学意义(F=126.753,P=0.000); 进一步两两比较,模型对照组软骨细胞凋亡率大于正常对照组和电针治疗组(LSD-t=3.769,P=0.000; LSD-t=7.000,P=0.000),电针治疗组软骨细胞凋亡率大于正常对照组(LSD-t=10.769,P=0.000)。结论:电针治疗可以通过抑制软骨细胞凋亡,减轻膝骨关节炎软骨的损伤,这可能是电针治疗膝骨关节炎的机制之一。
Abstract:
Objective:To observe the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis(KOA),and to explore the mechanisms of electroacupuncture on KOA.Methods:Thirty healthy New Zealand white rabbits were randomly divided into 3 groups as normal control group,model control group and electroacupuncture group,10 cases in each group.Left knee joints of experimental rabbits in model control group and electroacupuncture group were all built models through Hulth method.At the 5th week after modeling,cases in electroacupuncture group were administrated with electroacupuncture,while the others in the normal control group and model control group were administrated with normal feeding without treatment.After 4 weeks of treatment,all of the experimental rabbits were executed for making slices of left femoral condyle which were observed on the morphous of cartilaginous tissue and chondrocyte apoptosis situation.Results:①The morphous of cartilaginous tissue:the situations as smooth cartilage surface,clearness in four-layer structure,evenly distribution of chondrocytes without clustering and complete tidemark were found in normal control group; the situations as decrease in articular cartilage thickness,rough surface,significant fibrosis and fissures,clustered chondrocytes and fractured tidemark were found in model control group; and the situations as decrease in articular cartilage thickness,unsmooth surface,rare fibrosis and fissures,clustered chondrocytes and fractured tidemark were found in electroacupuncture group.②Chondrocyte apoptosis situation:there were statistical difference in chondrocyte apoptosis rate of experimental rabbits among the 3 groups(F=126.753,P=0.000).Chondrocyte apoptosis rate of model control group was higher than that of normal control group and electroacupuncture group respectively(LSD-t=3.769,P=0.000; LSD-t=7.000,P=0.000),and the chondrocyte apoptosis rate of electroacupuncture group was higher than that of normal control group(LSD-t=10.769,P=0.000).Conclusion:Electroacupuncture can relieve articular cartilage injuries of KOA through inhibiting chondrocyte apoptosis,which may be one of the mechanisms of electroacupuncture on KOA.

参考文献/References:

[1] Jubb RW,Tukmachi ES,Jones PW,et al.A blinded randomised trial of acupuncture(manual and electroacupuncture)compared with a non-penetrating sham for the symptoms of osteoarthritis of the knee[J].Acupunct Med,2008,26(2):69-78.
[2] Itoh K,Hirota S,Katsumi Y,et al.A pilot study on using acupuncture and transcutaneous electrical nerve stimulation(TENS)to treat knee osteoarthritis(OA)[J].Chin Med,2008,3:2.
[3] Witt CM,Jena S,Brinkhaus B,et al.Acupuncture in patients with osteoarthritis of the knee or hip:a randomized,controlled trial with an additional nonrandomized arm[J].Arthritis Rheum,2006,54(11):3485-3493.
[4] Vas J,Méndez C,Perea-Milla E,et al.Acupuncture as a complementary therapy to the pharmacological treatment of osteoarthritis of the knee:randomised controlled trial[J].BMJ,2004,329(7476):1216.
[5] Berman BM,Lao L,Langenberg P,et al.Effectiveness of acupuncture as adjunctive therapy in osteoarthritis of the knee:a randomized,controlled trial[J].Ann Intern Med,2004,141(12):901-910.
[6] Hulth A,Lindberg L,Telhag H.Experimental osteoarthritis in rabbits.Preliminary report[J].Acta Orthop Scand,1970,41(5):522-530.
[7] Moskwitz RW,Altman RD,Buckwalter JA,et al.Osteoarthritis:diagnosis and medical/surgical management[M].3rd ed.Philadelphia:Lippincott Williams&Wilkins,2001:1.
[8] 王瑞辉,杨介宾.电针对佐剂性关节炎大鼠IL-1和IL-6活性的影响[J].陕西中医学院学报,2001,24(4):43-45.
[9] 丘德兴.不同针灸法对膝骨性关节炎疗效对比的实验与临床研究[D].成都:成都中医药大学,2004.
[10] 郑秀存.针刺、电针和温针治疗对硒鼓性关节炎(KOA)软骨基质代谢调节作用的实验研究[D].成都:成都中医药大学,2005.
[11] 翁文水.针灸治疗家兔膝骨关节骨性关节炎的实验研究[D].福州:福建中医学院,2004.
[12] Blanco FJ,Guitian R,Vázquez-Martul E,et al.Osteoarthritis chondrocytes die by apoptosis.A possible pathway for osteoarthritis pathology[J].Arthritis Rheum,1998,41(2):284-289.
[13] Aigner T,Hemmel M,Neureiter D,et al.Apoptotic cell death is not a widespread phenomenon in normal aging and osteoarthritis human articular knee cartilage:a study of proliferation,programmed cell death(apoptosis),and viability of chondrocytes in normal and osteoarthritic human knee cartilage[J].Arthritis Rheum,2001,44(6):1304-1312.
[14] Watrin-Pinzano A,Etienne S,Grossin L,et al.Increased apoptosis in rat osteoarthritic cartilage corresponds to degenerative chondral lesions and concomitant expression of caspase-3[J].Biorheology,2006,43(3-4):403-412.
[15] Kim HA,Lee YJ,Seong SC,et al.Apoptotic chondrocyte death in human osteoarthritis[J].J Rheumatol,2000,27(2):455-462.
[16] Hashimoto S,Takahashi K,Amiel D,et al.Chondrocyte apoptosis and nitric oxide production during experimentally induced osteoarthritis[J].Arthritis Rheum,1998,41(7):1266-1274.
[17] 吴明霞,李西海,吴广文,等.电针后血清对肿瘤坏死因子α诱导软骨细胞凋亡的影响[J].中国组织工程研究与临床康复,2011,15(46):8551-8555.

相似文献/References:

[1]孟维娜,明立功,王新德,等.关节镜下清理联合腓骨近1/3段截骨治疗膝骨关节炎[J].中医正骨,2015,27(11):40.
[2]明立功,孟维娜,王新德,等.腓骨近端截骨治疗内侧间室膝骨关节炎的近期疗效观察[J].中医正骨,2015,27(10):25.
[3]张杰,王人彦,张玉柱.膝骨关节炎的治疗进展[J].中医正骨,2015,27(10):68.
[4]梁朝,蔡静怡,闫立,等.针刀疗法改善膝骨关节炎早期疼痛症状的疗效评价[J].中医正骨,2015,27(09):9.
　LIANG Zhao,CAI Jingyi,YAN Li,et al.Evaluation of the curative effect of needle-knife therapy for relieving knee pain in patients with early knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):9.
[5]王建武,党建军,李强,等.四联疗法治疗膝骨关节炎[J].中医正骨,2015,27(08):44.
[6]刘红娟,郭会利,郭树农.云克联合中药治疗膝骨关节炎的护理[J].中医正骨,2015,27(08):75.
[7]陈卫衡.探索建立系统的膝骨关节炎中医临床科研范式 和理论体系[J].中医正骨,2015,27(07):1.
[8]郑春松,叶蕻芝,李西海,等.透骨消痛胶囊中补肾柔肝药和活血祛风药治疗 骨关节炎作用方式的计算机模拟比较[J].中医正骨,2015,27(07):6.
　ZHENG Chunsong,YE Hongzhi,LI Xihai,et al.Comparison of the mode of action of Bushen Rougan(补肾柔肝)drugs versus Huoxue Qufeng(活血祛风)drugs contained in Tougu Xiaotong Jiaonang(透骨消痛胶囊)for the treatment of osteoarthritis:A computer simulation study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):6.
[9]帅波,沈霖,杨艳萍,等.加味青娥丸治疗膝骨关节炎的作用机制研究[J].中医正骨,2015,27(07):15.
　SHUAI Bo,SHEN Lin,YANG Yanping,et al.Study on the mechanism of action of Jiawei Qing'e Wan(加味青娥丸)for the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):15.
[10]梅其杰,袁长深,段戡,等.壮药骨痹方烫熨联合运动疗法治疗膝骨关节炎的临床研究[J].中医正骨,2015,27(07):27.
　MEI Qijie,YUAN Changshen,DUAN Kan,et al.Clinical study of the curative effect of hot compressing and rubbing with packet of Gubi Fang(骨痹方)combined with exercise therapy in the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):27.

备注/Memo

备注/Memo:
2011-08-17收稿 2012-02-14修回
更新日期/Last Update: 2012-04-30