[1]刘文刚,何伟,许学猛,等.兔激素性股骨头坏死与肝细胞色素P4503A酶活性的相关性研究[J].中医正骨,2011,23(10):6-9.
　LIU Wen-gang*,HE Wei,XU Xue-meng,et al.A study on the correlation between steroid-induced avascular necrosis of femoral head in rabbits and activity of cytochrome P4503A enzyme in liver cell[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(10):6-9.
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兔激素性股骨头坏死与肝细胞色素P4503A酶活性的相关 性研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期数:
2011年10期
页码:
6-9
栏目:
基础研究
出版日期:
2011-10-31

文章信息/Info

Title:
A study on the correlation between steroid-induced avascular necrosis of femoral head in rabbits and activity of cytochrome P4503A enzyme in liver cell
作者:
刘文刚1; 何伟2; 许学猛1; 吴淮1
1.广东省第二中医院,广东 广州 510095; 2.广州中医药 大学第一附属医院,广东 广州 510405
Author(s):
LIU Wen-gang*; HE Wei; XU Xue-meng; et al.*
The Second Traditional Chinese Medical Hospital of Guangdong Provinve,Guangzhou 510095,Guangdong,China
关键词:
股骨头坏死 肝细胞色素P4503A酶 酶诱导 动物实验
Keywords:
Femur head necrosis;  Cytochrome P4503A enzymes;  Enzyme induction;  Animal experimentation
摘要:
目的:探讨兔激素性股骨头坏死与肝细胞色素P4503A酶活性的相关性。方法:将18只SPF级健康新西兰家兔随机分成3组,即空白对照组、诱导剂组、抑制剂组,每组6只。空白对照组以生理盐水灌胃,诱导剂组肌肉注射苯巴比妥注射液,抑制剂组以伊曲康唑混悬液灌胃。连续用药3周后,测定血清肝细胞色素P4503A酶活性,并采用肌注甲泼尼龙琥珀酸钠方法造模。继续用药3周后处死动物,制作并观察病理组织标本,计算股骨头坏死面积和坏死率。结果:①肝细胞色素P4503A酶活性。空白对照组用药前后肝细胞色素P4503A酶活性比较,差异无统计学意义(t=1.859,P=0.122); 诱导剂组用药后肝细胞色素P4503A酶活性升高(t=7.482,P=0.002); 抑制剂组用药后肝细胞色素P4503A酶活性降低(t=5.060,P=0.004)。用药前3组肝细胞色素P4503A酶活性比较,差异无统计学意义(F=1.428,P=0.259); 用药后3组肝细胞色素P4503A酶活性比较,差异有统计学意义(F=8.577,P=0.001); 两两比较,诱导剂组高于空白对照组和抑制剂组(P=0.018,P=0.003),抑制剂组低于空白对照组(P=0.045)。②股骨头坏死面积。3组家兔股骨头坏死面积比较,差异有统计学意义(F=5.289,P=0.005)。组间两两比较,诱导剂组坏死面积小于空白对照组和抑制剂组(P=0.024,P=0.008),抑制剂组大于空白对照组(P=0.039)。③股骨头坏死率。3组家兔股骨头坏死率比较,差异有统计学意义(F=4.831,P=0.007)。组间两两比较,诱导剂组坏死率小于空白对照组和抑制剂组(P=0.045,P=0.015),抑制剂组大于空白对照组(P=0.031)。④肝细胞色素P4503A酶活性与股骨头坏死面积及坏死率的相关性。肝细胞色素P4503A酶活性与股骨头坏死面积和坏死率均呈负相关(r=-0.740,P=0.001; r=-0.753,P=0.001)。结论:肝细胞色素P4503A酶在兔激素性股骨头坏死中起重要作用,其活性与兔激素性股骨头坏死的发生呈显著负相关。
Abstract:
Objective:To explore the correlation between steroid-induced avascular necrosis of femoral head(SANFH)in rabbits and activity of cytochrome P4503A enzymes(CYP3A)in liver cell.Methods:Eighteen healthy SPF New Zealand rabbits were randomly divided into 3 groups as blank control group,inducer group and inhibitor group,6 cases in each group.Rabbits in blank control group were intragastric administrated with physiological saline,rabbits in inducer group were injected into muscle with phenobarbital,while the others in inhibitor group were intragastric administrated with itraconazole suspension.After 3 weeks of continuous administration,the enzyme activity of serum CYP3A was determined and intramuscular injection of methylprednisolone sodium succinate was used to make models.After the next 3 weeks of continuous administration,the rabbits were sacrificed for making pathological tissue specimens which were observed to measure the rates and areas of femoral head necrosis.Results:①The enzyme activity of CYP3A:There was no statistical difference in the rabbits of blank control group before and after the administration(t=1.859,P=0.122).After administration,the enzyme activity of CYP3A of inducer group enhanced(t=7.482,P=0.002),while that of inhibitor group depressed(t=5.060,P=0.004).There was no statistical difference in the enzyme activity of CYP3A among the 3 groups before administration(F=1.428,P=0.259),while there was statistical difference among the 3 groups after administration(F=8.577,P=0.001).The enzyme activity of CYP3A of inducer group was higher than that of blank control group and inhibitor group(P=0.018,P=0.003),and the enzyme activity of CYP3A of inhibitor group was lower than that of blank control group(P=0.045).②The areas of femoral head necrosis:There was statistical difference among the 3 groups(F=5.289,P=0.005).The areas of femoral head necrosis of inducer group were smaller than those of blank control group and inhibitor group(P=0.024,P=0.008),and the areas of femoral head necrosis of inhibitor group were bigger than that of blank control group(P=0.039).③The rate of femoral head necrosis:There was statistical difference among the 3 groups(F=4.831,P=0.007).The rate of inducer group was lower than that of blank control group and inhibitor group(P=0.045,P=0.015),and the rate of inhibitor group was higher than that of blank control group(P=0.031).④The correlations between CYP3A enzyme activity and the areas and rate of femoral head necrosis:There was a negative correlation between CYP3A enzyme activity and the areas and rate of femoral head necrosis(r=-0.740,P=0.001; r=-0.753,P=0.001).Conclusion:CYP3A enzyme in liver cell plays an important role in the steroid-induced avascular necrosis of femoral head in rabbits,and its activity has a significant negative correlation with the occurrence of steroid-induced avascular necrosis of femoral head in rabbits.

参考文献/References:

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更新日期/Last Update: 1900-01-01