[1]杜深星,徐守宇,储利胜,等.细胞外三磷酸腺苷对大鼠桡骨骨折愈合的影响[J].中医正骨,2011,23(05):27-31.
　DU Shen-xing*,XU Shou-yu,CHU Li-sheng,et al.Effect of extracellular ATP on the radial fracture repair in rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(05):27-31.
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细胞外三磷酸腺苷对大鼠桡骨骨折愈合的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期数:
2011年05期
页码:
27-31
栏目:
基础研究
出版日期:
2011-05-30

文章信息/Info

Title:
Effect of extracellular ATP on the radial fracture repair in rats
作者:
杜深星1; 徐守宇1; 2; 储利胜1; 何帮剑2
1.浙江中医药大学,浙江 杭州 310053;
2.浙江中医药大学附属第三医院,浙江 杭州 310005
Author(s):
DU Shen-xing*;  XU Shou-yu;  CHU Li-sheng;  et al.*
Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053,Zhejiang,China
关键词:
三磷酸腺苷 骨折 再生 大鼠
Keywords:
Adenosine Triphosphate;  Fracture;  Regeneration;  Rat
摘要:
目的:研究细胞外三磷酸腺苷(Adenosine triphosphate,ATP)对大鼠桡骨骨折愈合的影响。方法:100只SD大鼠随机平均分成ATP组和对照组,各组大鼠均采用顾氏造模法造成右侧桡骨骨折模型。ATP组于造模时断端注入2 mmoL·L-1的ATP,术后第1天、1周、2周、4周断端注入4 mmoL·L-1的ATP,剂量均为0.05 mL·100 g-1,对照组断端注入等体积生理盐水。术后第1周、2周、4周、6周取材,进行放射学、组织学、生物力学检查。结果:放射学检查术后第4周ATP组大多数骨折线趋于消失,其中有60%的标本显示骨折线完全消失,骨痂密度与骨密质基本相同,达到骨性愈合; 对照组骨折线模糊,骨折断端有骨痂填充,且大多数已桥接。术后第6周ATP组骨折线基本消失,骨痂密度与骨密质基本相同,达到骨性愈合; 对照组骨折线趋于消失,骨折断端被骨痂填充并桥接。组织学检查术后第4周ATP组骨折断端骨性骨痂融合形成板层骨,不规则骨髓腔亦融合形成新的再通的骨髓腔,达到骨性愈合; 对照组断端可见大量骨性骨痂,形成不规则的骨髓腔。生物力学测定术后第4周和第6周ATP组骨痂的最大载荷、最大位移和弹性模量与对照组比较,差异有统计学意义(P<0.01)。结论:细胞外ATP具有促进骨折愈合的作用。
Abstract:
Objective:To study the effect of extracellular ATP on the radial fracture repair in rats.Methods:100 SD rats were randomly divided into two groups: the ATP group(n=50)and the control group(n=50). All rats were established into right radius fracture rats models and the ATP group were injected 2 mmoL·L-1 ATP(0.05 mL·100 g-1)through fracture site in the same time. Then the ATP group were injected 4 mmol·L-1 ATP(0.05 mL·100 g-1)on these days: the day after the operation, 1 week after the operation, 2 weeks after the operation and 4 weeks after the operation. The control group were injected equal volume of NS through fracture site on the time listed above. All the rats were taken postoperative radiographic examination, histological examination and biomechanics measurements on these days: 1 week after the operation, 2 weeks after the operation, 4 weeks after the operation and 6 weeks after the operation.Results:The result of postoperative radiographic examination for the ATP group 4 weeks after the operation was that most fracture line tended to disappear and 60% of rat models in the ATP group showed a complete disappearance of their fracture lines with the density of poroma closing to the bone cortex which means the bones were basically healed. The control group's fracture lines tended to be blurred. The fracture site was filled with callus and most of them had already been bridged. After 6 weeks, the fracture lines of ATP group were disappeared basically with the density of poroma closing to the bone cortex. Fracture lines of the control group tend to disappear. The fracture site was filled with callus and had been bridged. After 4 weeks, histological examination showed that the osseouscallus formed into lamellar bone, and the marrow cavity reascularized. The fracture site of the control group showed that a lot of osseouscallus formed into irregular bone medullary cavity. Biomechanics measurements after 4 weeks and 6 weeks show that the poroma of the ATP group has a maximum of load, the biggest displacement and the greatest elastic modulus(P<0.01)compared to the control group.Conclusion:Extracellular ATP can promote fracture repairment.

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备注/Memo

备注/Memo:
基金项目:浙江省科技厅“新苗人才计划”资助项目(2010R410010)
通讯作者:储利胜 E-mail:chulisheng@21cn.com
更新日期/Last Update: 2011-05-30