[1]温博智,陈月峰,王宝剑,等.肠道菌群与颈椎病因果关系的双向孟德尔随机化分析[J].中医正骨,2024,36(12):43-48.
　WEN Bozhi,CHEN Yuefeng,WANG Baojian,et al.Investigation on the causal relationship between gut microbiota and cervical spondylosis:a bidirectional mendelian randomization analysis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2024,36(12):43-48.
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肠道菌群与颈椎病因果关系的双向孟德尔随机化分析()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第36卷
期数:
2024年12期
页码:
43-48
栏目:
数据库研究
出版日期:
2024-12-20

文章信息/Info

Title:
Investigation on the causal relationship between gut microbiota and cervical spondylosis:a bidirectional mendelian randomization analysis
作者:
温博智; 陈月峰; 王宝剑; 马睿辰; 戴文康; 马玉峰
北京中医药大学第三附属医院,北京 100029
Author(s):
WEN Bozhi; CHEN Yuefeng; WANG Baojian; MA Ruichen; DAI Wenkang; MA Yufeng
The Third Affiliated Hospital of Beijing University of Traditional Chinese Medicine,Beijing 100029,China
关键词:
颈椎病;  痹证;  脾(中医);  肠道微生物群;  孟德尔随机化分析
Keywords:
cervical spondylosis;  arthromyodynia;  spleen(TCM);  gastrointestinal microbiome;  Mendelian randomization analysis
摘要:
目的:探讨肠道菌群与颈椎病的因果关系。方法:分别从IEU OpenGWAS project数据库和MiBioGen数据库获取颈椎病和肠道菌群的全基因组关联研究数据,从中筛选符合要求的单核苷酸多态性(single nucleotide polymorphism,SNP)位点作为工具变量进行两样本双向孟德尔随机化(Mendelian randomization,MR)分析。采用逆方差加权法(inverse variance weighted,IVW)作为主要分析方法,并采用MR-Egger、加权中位数法、加权模式、简单模式进行补充分析。采用Cochran's Q检验对IVW和MR-Egger法的分析结果进行异质性检验,采用MR-Egger回归进行水平多效性检验,采用留一法进行敏感性分析。结果:共筛选出颈椎病的SNP位点70个; 肠道菌群的SNP位点46个,其中δ-变形菌纲11个、肠杆菌科8个、瘤胃球菌属UCG011 7个、狭义梭菌属4个、粪球菌属8个、瘤胃梭菌属8个。正向MR分析结果显示,δ-变形菌纲与颈椎病存在负向因果关系,狭义梭菌属、粪球菌属与颈椎病存在正向因果关系; 反向MR分析结果显示,颈椎病与肠道菌群之间不存在明显的因果关系。水平多效性检验和异质性检验结果显示,不存在水平多效性和异质性; 留一法分析结果显示,无明显离群值,提示MR分析结果稳健。结论:肠道菌群中的δ-变形菌纲、狭义梭菌属、粪球菌属与颈椎病存在可靠的因果关系。
Abstract:
Objective:To investigate the causal relationship between gut microbiota(GM)and cervical spondylosis(CS).Methods:The genome-wide association study(GWAS)datasets about GM and CS were extracted from the IEU OpenGWAS project database and MiBioGen database,respectively.The eligible single nucleotide polymorphism(SNP)loci were screened as instrumental variables for two-sample bidirectional mendelian randomization(MR)analysis.Inverse variance weighted(IVW)were selected as the main analysis method,and MR-Egger,weighted median estimator(WME),weighted mode(WM)and simple mode(SM)as supplemental analysis method.Cochran's Q test was performed on the heterogeneity of the results analyzed by IVW method and MR-Egger method.In addition,the horizontal pleiotropy was examined by MR-Egger regression,and the sensitivity of the MR analysis results was evaluated by leave-one-out(LOO)test.Results:Seventy CS SNP loci and 46 GM(11 δ-proteobacteria,8 enterobacteriaceae,7 ruminococcus UCG011,4 clostridium sensu stricto,8 coprococcus,and 8 ruminal clostridium)SNP loci were included and served as the instrumental variables.The results of forward MR analysis showed an inverse causal relationship between δ-proteobacteria and CS,and a positive causal relationship between clostridium sensu stricto,coprococcus and CS.The results of reverse MR analysis showed that there was no obvious causal relationship between CS and GM.The results of horizontal pleiotropy test and heterogeneity test indicated that there was no horizontal pleiotropy and heterogeneity.The results of LOO test showed no outlier,suggesting the results of the MR analysis in this study were stable and reliable.Conclusion:GM,including δ-proteobacteria,clostridium sensu stricto and coprococcus,has a reliable causal relationship with CS.

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通讯作者:马玉峰 E-mail:mayf0826@163.com
更新日期/Last Update: 1900-01-01