[1]章家皓,田佳庆,王帅,等.补肾强筋胶囊对膝骨关节炎大鼠膝关节软骨下骨质的影响及其作用机制的实验研究[J].中医正骨,2024,36(08):19-26.
 ZHANG Jiahao,TIAN Jiaqing,WANG Shuai,et al.Effects of Bushen Qiangjin Jiaonang(补肾强筋胶囊)on knee subchondral bone in knee osteoarthritis rats and its mechanism of action:an experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2024,36(08):19-26.
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补肾强筋胶囊对膝骨关节炎大鼠膝关节软骨下骨质的影响及其作用机制的实验研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第36卷
期数:
2024年08期
页码:
19-26
栏目:
基础研究
出版日期:
2024-08-20

文章信息/Info

Title:
Effects of Bushen Qiangjin Jiaonang(补肾强筋胶囊)on knee subchondral bone in knee osteoarthritis rats and its mechanism of action:an experimental study
作者:
章家皓田佳庆王帅陈佳浩李聪聪刘文刚姜涛许学猛
广东省第二中医院,广东 广州 510030
Author(s):
ZHANG JiahaoTIAN JiaqingWANG ShuaiCHEN JiahaoLI CongcongLIU WengangJIANG TaoXU Xuemeng
The Second Traditional Chinese Medicine Hospital of Guangdong,Guangzhou 510030,Guangdong,China
关键词:
骨关节炎 大鼠Sprague-Dawley 补肾强筋胶囊 软骨下骨 H型血管
摘要:
目的:探讨补肾强筋胶囊对膝骨关节炎(knee osteoarthritis,KOA)大鼠膝关节软骨下骨质的影响及其作用机制。方法:6周龄雄性SPF级SD大鼠24只,随机分为正常组、模型组、补肾强筋胶囊组,每组8只。模型组、补肾强筋胶囊组采用前交叉韧带离断法对右后膝进行KOA造模。造模4周后,补肾强筋胶囊组大鼠以补肾强筋胶囊药液灌胃,正常组、模型组大鼠以去离子水灌胃,每日1次,共灌胃4周。灌胃4周后,处死大鼠,取出右后肢膝关节,观察大鼠膝关节标本的大体结构。采用Micro-CT扫描仪对大鼠膝关节标本进行扫描,分析各组大鼠膝关节骨组织体积分数、骨小梁数目、骨小梁厚度和骨小梁分离度。采用苏木精-伊红染色和番红O-固绿染色观察各组大鼠膝关节软骨下骨组织情况。采用免疫组织化学染色检测大鼠膝关节软骨下骨中低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、血管内皮生长因子A(vascular endothelial growth factor A,VEGFA)的表达情况,采用免疫荧光染色检测大鼠膝关节软骨下骨中CD31、Endomucin的表达情况。结果:①补肾强筋胶囊对KOA大鼠膝关节大体结构的影响。模型组膝关节软骨磨损严重,关节间隙明显狭窄,关节腔内结构破坏严重,周围组织粘连明显; 与模型组相比,补肾强筋胶囊组膝关节软骨磨损较轻,关节间隙轻度狭窄。②补肾强筋胶囊对KOA大鼠膝关节软骨下骨质的影响。MicroCT检查结果显示,模型组膝关节软骨下骨小梁排列稀疏,周围可见明显骨赘; 补肾强筋胶囊组膝关节软骨下骨小梁排列较模型组紧密。与正常组相比,模型组和补肾强筋胶囊组膝关节软骨下骨组织体积分数小(P=0.001,P=0.012),骨小梁数目少(P=0.013,P=0.034),骨小梁厚度和骨小梁分离度大(P=0.000,P=0.001; P=0.000,P=0.005)。与模型组相比,补肾强筋胶囊组膝关节软骨下骨组织体积分数大(P=0.001),骨小梁数目多(P=0.039),骨小梁厚度和骨小梁分离度小(P=0.022,P=0.039)。苏木精-伊红染色和番红O-固绿染色显示,模型组和补肾强筋胶囊组膝关节软骨退变明显,软骨下骨小梁疏松,但补肾强筋胶囊组软骨下骨钙化程度较模型组低。③补肾强筋胶囊对KOA大鼠膝关节软骨下骨中H型血管形成相关蛋白表达的影响。模型组和补肾强筋胶囊组膝关节软骨下骨中HIF-1α、VEGFA、CD31、Endomucin的表达量均高于正常组(P=0.010,P=0.002,P=0.041,P=0.017; P=0.045,P=0.025,P=0.032,P=0.011)。补肾强筋胶囊组膝关节软骨下骨中HIF-1α、VEGFA、CD31、Endomucin的表达量均低于模型组(P=0.026,P=0.006,P=0.036,P=0.029)。结论:补肾强筋胶囊可抑制KOA大鼠膝关节软骨下骨中H型血管形成相关蛋白的表达,干预H型血管的形成,从而改善软骨下骨质。

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备注/Memo

备注/Memo:
基金项目:广州地区中西医协同临床重大创新技术建设项目(穗卫函〔2023〕2318号); 广州市科技计划项目(202206010048)
通讯作者:许学猛 E-mail:xuxuemeng@163.com
更新日期/Last Update: 1900-01-01