[1]赵祚塨,王孝辉,姚俊娜,等.疏血通注射液预防大鼠血管吻合口血栓形成的实验研究[J].中医正骨,2015,27(03):12-16.
　ZHAO Zuogong,WANG Xiaohui,YAO Junna,et al.An experimental study of Shuxuetong(疏血通)injection for prevention of vascular anastomotic stoma thrombosis in rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):12-16.
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疏血通注射液预防大鼠血管吻合口血栓形成的实验研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期数:
2015年03期
页码:
12-16
栏目:
基础研究
出版日期:
2015-03-30

文章信息/Info

Title:
An experimental study of Shuxuetong(疏血通)injection for prevention of vascular anastomotic stoma thrombosis in rats
作者:
赵祚塨; 王孝辉; 姚俊娜; 郭绍勇; 陈利国; 段卫峰; 穆世民; 杜志军; 蔡利涛
河南省洛阳正骨医院/河南省骨科医院,河南 洛阳 471002
Author(s):
ZHAO Zuogong; WANG Xiaohui; YAO Junna; GUO Shaoyong; Chen Liguo; DUAN Weifeng; MU Shimin; DU Zhijun; CAI Litao
Luoyang Orthopedic-Traumatological Hospital,Luoyang 471002,Henan,China
关键词:
血栓形成;  疏血通;  肝素;  显微外科手术;  血小板;  纤维蛋白;  内皮细胞;  动物实验
Keywords:
thrombosis;  Shuxuetong;  heparin;  microsurgery;  blood platelets;  fibrin;  endothelial cells;  animal experimentation
摘要:
目的:观察疏血通注射液预防大鼠血管吻合口血栓形成的疗效。方法:将120只Wistar大鼠随机分为对照组、疏血通组及肝素钠组,每组40只。对照组、疏血通组及肝素钠组大鼠分别以生理盐水(10 mL·kg-1)、疏血通注射液(2 g·kg-1)及肝素钠注射液(600 U·kg-1)进行腹腔注射。药物注射6 h后将所有大鼠左侧颈总动脉切断,并即刻对其进行端端吻合。分别于造模结束后30 min及1、3、7 d时从各组随机选取10只大鼠,切取包含吻合口远近端各0.5 cm的颈总动脉,在电子显微镜下观察并计算吻合口及缝线表面覆盖的纤维蛋白、血小板及内皮细胞的百分比。结果:①纤维蛋白覆盖率。造模后不同时间,3组大鼠血管吻合口纤维蛋白覆盖率比较,组间差异均有统计学意义(F=133.802,P=0.000; F=18.741,P=0.000; F=212.943,P=0.000; F=341.174,P=0.000)。造模后30 min时对照组纤维蛋白覆盖率低于疏血通组和肝素钠组(P=0.000,P=0.000); 造模后1、3、7 d时对照组纤维蛋白覆盖率均高于疏血通组和肝素钠组(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000); 造模后3 d时疏血通组纤维蛋白覆盖率高于肝素钠组(P=0.000); 其余各时点疏血通组和肝素钠组比较,组间差异均无统计学意义。②血小板覆盖率。造模后不同时间,3组大鼠血管吻合口血小板覆盖率比较,组间差异均有统计学意义(F=352.002,P=0.000; F=84.734,P=0.000; F=368.903,P=0.000; F=14.413,P=0.000)。造模后30 min、1 d、3 d、7 d时对照组血小板覆盖率均高于疏血通组和肝素钠组(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000); 造模结束后30 min时疏血通组血小板覆盖率高于肝素钠组(P=0.027); 其余各时点疏血通组和肝素钠组比较,组间差异均无统计学意义。③内皮细胞覆盖率。除造模后30 min时外,其余各时点3组大鼠血管吻合口内皮细胞覆盖率比较,组间差异均有统计学意义(F=0.000,P=1.000; F=835.502,P=0.000; F=363.874,P=0.000; F=780.410,P=0.000)。造模后1、3、7 d时对照组内皮细胞覆盖率均低于疏血通组和肝素钠组(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000); 造模后1、7 d时疏血通组内皮细胞覆盖率均高于肝素钠组(P=0.002,P=0.001); 造模后3 d时疏血通组和肝素钠组比较,组间差异无统计学意义。结论:疏血通注射液能促进内皮细胞生长,减少血小板在血管吻合口的堆积,从而避免血栓形成,其疗效与肝素钠相当。
Abstract:
Objective:To observe the preventative effect of Shuxuetong(疏血通)injection on vascular anastomotic stoma thrombosis in rats.Methods:One hundred and twenty Wistar rats were randomly divided into control group,Shuxuetong group and heparin group,40 cases in each group.The Wistar rats in the 3 groups were intraperitoneal injected with normal saline(10 mL/kg),Shuxuetong injection(2 g/kg)and heparin injection(600 U/kg)respectively.The left common carotid arteries(LCCA)of all rats were cut off at 6 hours after injection,and end-to-end anastomosis were performed instantly.Ten rats were randomly selected from each group at 30 minutes,1,3 and 7 days after the modeling respectively,and the common carotid arteries near the anastomotic stoma were fetched for observing.The fraction of coverage of fibrin,blood platelets and endothelial cells at anastomotic stoma and suture surface were observed and calculated by using electron microscope.Results:There was statistical difference in coverage fraction of fibrin in vascular anastomotic stoma between the 3 groups after the modeling(F=133.802,P=0.000; F=18.741,P=0.000; F=212.943,P=0.000; F=341.174,P=0.000).The coverage fraction of fibrin of control group was lower than that of Shuxuetong group and heparin group at 30 minutes after the modeling(P=0.000,P=0.000).The coverage fraction of fibrin of control group was higher than that of Shuxuetong group and heparin group at 1,3 and 7 days after the- modeling(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000).The coverage fraction of fibrin of Shuxuetong group was higher than that of heparin group at 3 days after the modeling(P=0.000).There was no statistical difference in coverage fraction of fibrin between Shuxuetong group and heparin group at the rest timepoints.There was statistical difference in coverage fraction of blood platelets in vascular anastomotic stoma between the 3 groups after the modeling(F=352.002,P=0.000; F=84.734,P=0.000; F=368.903,P=0.000; F=14.413,P=0.000).The coverage fraction of blood platelets of control group was higher than that of Shuxuetong group and heparin group at 30 minutes and 1,3 and 7 days after the modeling(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000).The coverage fraction of blood platelets of Shuxuetong group was higher than that of heparin group at 30 minutes after the modeling(P=0.027).There was no statistical difference in coverage fraction of blood platelets between Shuxuetong group and heparin group at the rest timepoints.There was statistical difference in coverage fraction of endothelial cells in vascular anastomotic stoma between the 3 groups except at 30 minutes after the modeling(F=0.000,P=1.000; F=835.502,P=0.000; F=363.874,P=0.000; F=780.410,P=0.000).The coverage fraction of endothelial cells of control group was lower than that of Shuxuetong group and heparin group at 1,3 and 7 days after the modeling(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000).The coverage fraction of endothelial cells of Shuxuetong group was higher than that of heparin group at 1 and 7 days after the modeling(P=0.002,P=0.001).There was no statistical difference in coverage fraction of endothelial cells between Shuxuetong group and heparin group at 3 days after the modeling.Conclusion:Shuxuetong injection can promote the growth of endothelial cells and reduce the accumulation of blood platelets in vascular anastomotic stoma to avoid thrombogenesis,and it is similar to heparin in the total curative effect.

参考文献/References:

[1] 韩明通,方光荣.断指再植术后抗凝药物的应用[J].中华显微外科杂志,2012,35(4):347-350.
[2] 胡长青,冯亚高,付贯忠,等.利器致腕部完全离断七例[J].中华显微外科杂志,2012,35(2):122.
[3] 庄加川,李敏姣,吴耿,等.指动脉静脉化在末节断指再植中的临床应用[J].中华显微外科杂志,2012,35(1):78-79.
[4] 雷林革,刘刚义,何如祥,等.醉酒患者断指再植术后血管危象30例分析[J].中华显微外科杂志,2012,35(6):493-495.
[5] 王培吉,董启榕,易男.断指再植术发生血管危象的原因分析及防治措施[J].中华显微外科杂志,2010,33(1):19-22.
[6] 朱福海.疏血通的药理作用与临床应用[J].安徽医药,2006,10(11):873-875.
[7] 鲁鑫,权晓丹.疏血通的临床应用[J].黑龙江医药,2005,18(4):298-299.
[8] 程春生,查朱青,单海民,等.红花注射液对大鼠血管吻合口修复的影响[J].中医正骨,2006,18(12):3-5.
[9] 李继峰,顾玉东,钟慈声,等.药物及吸烟对大鼠血管吻合口内皮细胞愈合的影响[J].上海医科大学学报,2000,27(5):350-353.
[10] Ohki M,Ohki Y,Ishihara M,et al.Tissue type plasminogen activator regulates myeloid-cell dependent neoangiogenesis during tissue regeneration[J].Blood,2010,115(21):4302-4312.
[11] 种振岳,高聿同,朱磊,等.抗凝药物作用于小血管吻合口的电镜观察[J].山东医药,2001,41(24):17-19.
[12] Sahni A,Francis CW. Stimulation of endothelial cell proliferation by FGF-2 in the presence of fibrinogen requires alphavbeta3[J].Blood,2004,104(12):3635-3641.
[13] Mosesson MW.Fibrinogen and fibrin structure and functions [J].J Thromb Haemost,2005,3(8):1894-1904.
[14] Shiose S,Hata Y,Noda Y,et al.Fibrinogen stimulates in vitro angiogenesis by choroidal endothelial cells via autocrine VEGF[J].Graefes Arch Clin Exp Ophthalmol,2004,242(9):777-783.
[15] 赵风林,李宗宝,王传荣,等.断指再植术后患者血清丙二醛含量及超氧化物歧化酶活性变化[J].中华手外科杂志,2013,29(5):299-300.

备注/Memo

备注/Memo:
2014-09-03收稿 2015-01-19修回
通讯作者:赵祚塨 E-mail:407493048@qq.com
更新日期/Last Update: 2015-03-30