[1]蔡立雄,杨海韵,孙丙银,等.伤科黄水对兔牵拉成骨区新骨生成的影响[J].中医正骨,2014,26(10):12-15.
　Cai Lixiong*,Yang Haiyun,Sun Bingyin,et al.Effect of the traumatology yellow water on new bone formation of distraction osteogenesis zone in rabbit[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(10):12-15.
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伤科黄水对兔牵拉成骨区新骨生成的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年10期
页码:
12-15
栏目:
基础研究
出版日期:
2014-10-30

文章信息/Info

Title:
Effect of the traumatology yellow water on new bone formation of distraction osteogenesis zone in rabbit
作者:
蔡立雄1; 杨海韵1; 孙丙银2; 温建强1; 吴峰1; 吴玢2;  严加取1
1.广州省佛山市中医院,广东 佛山 528000;
2.广州中医药大学,广东 广州 510006
Author(s):
Cai Lixiong*; Yang Haiyun; Sun Bingyin; Wen Jianqiang; Wu Feng; Wu Bin; Yan Jiaqu.*
Foshan Hospital of Traditional Chinese Medicine,Foshan 528000,Guangdong,China
关键词:
骨生成; 牵张 伤科黄水 动物实验
Keywords:
Osteogenesis; distraction;  The traumatology yellow water;  Animal experimentation
摘要:
目的:探讨伤科黄水对兔牵拉成骨区新骨生成的影响及作用机制。方法:对24只新西兰白兔左侧股骨进行牵拉成骨手术,手 术成功后将所有兔子随机分为2组,每组12只。实验组手术切口处及克氏针针孔处外敷伤科黄水纱布,对照组切口处及克氏针针孔 处外敷酒精纱布。术后通过肉眼观察、血液学检查及X线检查,比较2组动物的切口和新骨生成情况。结果:①肉眼观察。术后3 d,实验组动物手术切口表面及克氏针针孔处色红、干燥、少许结痂、肌肉组织轻度肿胀; 术后5 d,切口表面干燥,远端克氏针针 孔处肤色淡红; 术后7 d,切口处有大量皮毛生长,切口已愈合,针孔处无感染迹象; 术后10 d,切口完全愈合,皮毛生长接近正常 。术后3 d,对照组动物切口色红、较湿润,远端有少许渗液,针孔处未见渗液,肌肉组织明显肿胀; 术后5 d,切口皮肤色红、轻度 湿润、肿胀,有少许结痂及皮毛生长; 术后7 d,远端针孔处有少许渗液,肌肉组织轻度肿胀,有中等量皮毛生长; 术后10 d,切口 结痂愈合,无明显肿胀,表面干燥,有中等量皮毛生长。实验组动物比对照组创面愈合快[(7.5±0.6)d,(8.3±1.2) d,t=-2.066,P=0.025]。②血液学检查。术后5 d,实验组肿瘤坏死因子α含量和碱性成纤维细胞生长因子含量均高于对照组 [(2.3±0.8)ng·mL-1,(1.8±0.5)ng·mL-1,t=1.836,P=0.040;(125.2±4.6) ng·L-1,(121.3±3.8)ng·L-1,t=2.264,P=0.017]; 术后7 d,实验组白细胞计数、中心粒细胞计 数和百分比、淋巴细胞计数和百分比均低于对照组[(7.3±0.3)个·mL-1,(8.7±1.7)个·mL- 1,t=-2.809,P=0.001;(4.0±0.9)个·mL-1,(5.1±1.6)个·mL-1,t=2.076,P=0.025; (54.8±1.7)%,(57.1±3.9)%,t=-1.873,P=0.037;(2.5±0.5)个·mL-1,(3.1±0.9)个 ·mL-1,t=2.019,P=0.028;(23.8±1.6)%,(25.3±1.4)%,t=2.444,P=0.011]。③X线检查。术后4周时的X线 片示,2组动物牵拉成骨区均可见灰色低密度影,有大量新骨形成,骨皮质尚未连续; 实验组牵拉成骨区骨小梁密度、数量明显多 于对照组,骨痂生长情况也优于对照组。结论:伤科黄水能促进兔牵拉成骨区新骨生成,其机制可能是伤科黄水改善了手术部位的 软组织条件。
Abstract:
Objective:To explore the effect and its mechanism of action of the traumatology yellow water on new bone formation of distraction osteogenesis(DO)zone in rabbit.Methods:Twenty-four New Zealand white rabbits were obtained for DO surgery in the left femurs,after the surgery all the rabbits enrolled in the study were randomly divided into 2 groups,12 cases in each group.The traumatology yellow water gauze(experimental group) and alcohol gauze(control group)were applied to the surgical incisions and Kirschner wires pinholes of rabbits.After the surgery,the rabbits were compared between the 2 groups in incisions and new bone formation by the means of macroscopic observation,hematological examination and X-ray examination.Results:Three days after surgery,the surgical incisions and Kirschner wires pinholes of the rabbits in experimental group showed red,dry,a little callus and mild swelling.Five days after surgery,the incision surface were dry and the color of skin around distal Kirschner wires pinholes were reddish.Seven days after surgery, a lot of newborn fur were found around the incisions and the incisions had healed up and there was no evidence of infection in pinholes.Ten days after surgery,the incisions healed completely and the growth of fur approach normal.Three days after surgery,the surgical incisions of the rabbits in control group were red and wet and there was a few seepage in the distal end of incisions.No seepage was found in pinholes and obvious swelling was found in muscular tissue.Five days after surgery,the incisions skin were red,mild wet and swollen and a little callus and fur were found around it.Seven days after surgery,there were a few seepage in distal pinholes and the muscular tissue was mild swollen and the amount of fur was moderate.Ten days after surgery,the incisions healed without obvious swelling,The surface of incision was dry and moderate amount of fur was found around the incision.The incisions healing time of rabbits in experimental group was shorter than that of the control group(7.5+/-0.6 vs 8.3+/-1.2 days,t=-2.066,P=0.025).Five days after surgery,the content of tumor necrosis factor-α and basic fibroblast growth factor of experimental group were higher than thoset of control group(2.3+/-0.8 vs 1.8+/-0.5 ng/ml,t=1.836,P=0.040; 125.2+/-4.6 vs 121.3+/-3.8 ng/l,t=2.264,P=0.017).Seven days after surgery,the white blood cell count,neutrophils count and percentage,lymphocyte count and percentage of the experimental group were lower than those of control group(7.3+/-0.3 vs 8.7+/-1.7 cell/ml,t=-2.809,P=0.001; 4.0+/-0.9 vs 5.1+/-1.6 cell/ml,t=2.076,P=0.025; 54.8+/-1.7 vs 57.1+/-3.9%,t=-1.873,P=0.037; 2.5+/-0.5 vs 3.1+/-0.9 cell/ml,t=2.019,P=0.028; 23.8+/-1.6 vs 25.3+/-1.4%,t=2.444,P=0.011).Four weeks after surgery the X- ray film showed that the gray low density shadow could be found in DO zone in the 2 groups,moreover,there were a lot of newborn bone and the cortical bone was discontinuous.The density and amount of the bone trabecula in DO zone of experimental group were significantly more than those of the control group,and also the same to bony callus growth.Conclusion:The traumatology yellow water can promote the new bone formation in DO zone of rabbits,probably for that it can improve the soft tissue condition around the operative site.

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备注/Memo

备注/Memo:
基金项目:广东省中医药管理局科研项目(20130157)
通讯作者:杨海韵 E-mail:13809210960@139.com
更新日期/Last Update: 2014-10-30