[1]唐萌芽,倪慧英,张学民,等.补肾活血中药对膝骨关节炎患者经典Wnt/β-catenin通路调控作用的临床研究[J].中医正骨,2014,26(08):12-14.
　Tang Mengya*,Ni Huiying,Zhang Xuemin,et al.Clinical study on the role of reinforcing kidney activating blood drugs(TCD)in the regulation of classic Wnt/β-catenin pathway in patients with osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(08):12-14.
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补肾活血中药对膝骨关节炎患者经典Wnt/β-catenin通路调控 作用的临床研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年08期
页码:
12-14
栏目:
临床研究
出版日期:
2014-08-30

文章信息/Info

Title:
Clinical study on the role of reinforcing kidney activating blood drugs(TCD)in the regulation of classic Wnt/β-catenin pathway in patients with osteoarthritis
作者:
唐萌芽; 倪慧英; 张学民; 邵丽芳
浙江省富阳市中医骨伤医院,浙江 富阳 311400
Author(s):
Tang Mengya*; Ni Huiying; Zhang Xuemin; Shao Lifang
*Fuyang TCM Orthopedic-Traumatological Hospital,Fuyang 311400,Zhejiang,China
关键词:
骨关节炎; 膝 补肾活血 Wnt信号通路 β连环素 骨形态发生蛋白-2
Keywords:
Osteoarthritis; knee;  Reinforcing kidney activating blood;  Wnt signaling pathway;  Beta catenin;  BMP-2
摘要:
目的:观察补肾活血中药治疗膝骨关节炎的临床疗效及其作用机制。方法:纳入研究的膝骨关节炎患者30例,采用口服补肾活血中药治疗。比较治疗前及治疗后12周,膝骨关节炎患者的JOA评分和WOMAC评分; 并采用实时荧光定量聚合酶链式反应检测Wnt-5a、β-catenin、BMP-2 mRNA表达的变化情况。结果:治疗后12周,JOA评分较治疗前明显提高[(62.01±11.79)分,(80.90±6.90)分,t=4.767,P=0.001]; WOMAC评分较治疗前明显降低[(43.20±10.89)分,(19.20±5.43)分,t=4.535,P=0.002]; Wnt-5a、β-catenin、BMP-2 mRNA表达量较治疗前均降低(2.74±0.69,1.44±0.29,t=4.874,P=0.001; 3.21±1.13,1.45±0.32,t=5.574,P=0.001; 3.55±1.38,1.33±0.53,t=4.235,P=0.003)。结论:采用补肾活血中药治疗膝骨关节炎,能改善膝关节功能,缓解患者疼痛; 降低Wnt-5a、β-catenin、BMP-2 mRNA表达量,这可能是补肾活血中药防治骨关节炎的可能作用机制。
Abstract:
Objective:To observe the clinical curative effect of reinforcing kidney activating blood drugs(TCD)on knee osteoarthritis(KOA)and to study its mechanism of action.Methods:Thirty patients with KOA were enrolled in the study and were treated with oral reinforcing kidney activating blood drugs(TCD).The JOA scores and WOMAC scores were compared between pretreatment and post-treatment,and the expression of Wnt-5a mRNA,β-catenin mRNA and BMP-2 mRNA were detected through real-time fluorescence quantitative PCR.Results:After twelve-week treatment,the JOA scores increased significantly(62.01+/-11.79 vs 80.90+/-6.90 points,t=4.767,P=0.001),while the WOMAC scores decreased significantly(43.20+/-10.89 vs 19.20+/-5.43 points,t=4.535,P=0.002),and the expression of Wnt-5a mRNA,β-catenin mRNA and BMP-2 mRNA were down-regulated(2.74+/-0.69 vs 1.44+/-0.29,t=4.874,P=0.001; 3.21+/-1.13 vs 1.45+/-0.32,t=5.574,P=0.001; 3.55+/-1.38 vs 1.33+/-0.53,t=4.235,P=0.003).Conclusion:In the treatment of KOA,oral application of reinforcing kidney activating blood drugs(TCD)can improve the knee joint functions,relief the knee pain and down-regulate the expression of Wnt-5a mRNA,β-catenin mRNA and BMP-2 mRNA,so it may be the mechanism of action of reinforcing kidney activating blood drugs(TCD)in the prevention of KOA.

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更新日期/Last Update: 2014-08-30