[1]吴海洋.骨髓间充质干细胞移植对胶原诱导性关节炎大鼠血清细胞因子的影响[J].中医正骨,2019,31(08):14-19.
　WU Haiyang.Effects of bone marrow mesenchymal stem cells transplantation on serum cytokines in rats with collagen-induced arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2019,31(08):14-19.
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骨髓间充质干细胞移植对胶原诱导性关节炎大鼠血清细胞因子的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第31卷
期数:
2019年08期
页码:
14-19
栏目:
基础研究
出版日期:
2019-08-20

文章信息/Info

Title:
Effects of bone marrow mesenchymal stem cells transplantation on serum cytokines in rats with collagen-induced arthritis
作者:
吴海洋
(商丘市中心医院,河南 商丘 476000)
Author(s):
WU Haiyang
Shangqiu Central Hospital, Shangqiu 476000,Henan,China
关键词:
关节炎; 类风湿;  间质干细胞移植;  血管内皮生长因子类;  基质金属蛋白酶3;  肿瘤坏死因子α;  白细胞介素17;  动物实验;  大鼠
Keywords:
rthritis; rheumatoid;  mesenchymal stem cell transplantation;  vascular endothelial growth factors;  matrix metalloproteinase 3;  tumor necrosis factor-alpha;  interleukin-17;  animal experimentation;  rats
摘要:
目的:观察骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)移植对胶原诱导性关节炎(collagen induced arthritis,CIA)大鼠血清细胞因子的影响。方法:选取清洁级雌性Wistar大鼠60只。用颈椎脱臼断颈法处死1只大鼠,取股骨和胫骨骨髓,分离、培养BMSCs。其余59只大鼠采用随机数字表分为正常对照组(20只)、模型组(20只)、细胞移植组(19只)。模型组和细胞移植组每只大鼠在尾根部、背部及左后足跖部皮下注射含牛Ⅱ型胶原的乳化液共0.5 mL,正常对照组注射等量生理盐水。于第1次注射后第14 天再注射1次。于第2次注射后的第15天,各组分别随机处死2只大鼠,观察左侧踝关节组织形态,判断造模是否成功。于第2次注射的当天,细胞移植组大鼠通过尾静脉注射BMSCs悬液,正常对照组和模型组注射等量生理盐水。
Abstract:
Objective:To observe the effects of bone marrow mesenchymal stem cells(BMSCs)transplantation on serum cytokines in rats with collagen-induced arthritis(CIA).Methods:Sixty clean-grade female Wistar rats were selected.One rat was randomly selected out from the 60 rats and was executed by using cervical dislocation method,and its bone marrow were fetched out from femur and tibia for separating and culturing BMSCs.The other 59 rats were randomly divided into normal control group(20),model group(20)and BMSCs transplantation group(19)by using random digits table.The rats in model group and BMSCs transplantation group were subcutaneously injected with emulsified liquid containing bovine typeⅡcollagen with dose of 0.5 mL into their tail roots,backs and left posterior plantar areas,while the rats in normal control group were subcutaneously injected with the same dose of normal saline(NS)in the same places.The second subcutaneous injection was performed on rats at the 14th day after the first injection.At 15th day after the second injection,2 rats were randomly selected out from each group and were executed,and their left ankle-joints were fetched out and the tissue morphology of left ankle-joints was observed to determine whether the models were built successfully.On the day of the second injection,the rats in BMSCs transplantation group were injected with BMSCs suspension into caudal vein,while the rats in normal control group and model group were injected with the same dose of NS into caudal vein.

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备注/Memo

备注/Memo:
(收稿日期:2019-06-11 本文编辑:杨雅)
更新日期/Last Update: 2019-08-15