[1]洪郭驹.超低温冷冻同种异体软骨的研究进展[J].中医正骨,2021,33(03):49-52.
 HONG Guoju.Advancement of research on allogeneic cartilage cryopreservation technique[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2021,33(03):49-52.
点击复制

超低温冷冻同种异体软骨的研究进展()
分享到:

《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第33卷
期数:
2021年03期
页码:
49-52
栏目:
综 述
出版日期:
2021-03-20

文章信息/Info

Title:
Advancement of research on allogeneic cartilage cryopreservation technique
作者:
洪郭驹
(阿尔伯塔大学医学院,加拿大 埃德蒙顿 T6G 2R3)
Author(s):
HONG Guoju
关键词:
组织保存 低温保存 玻璃化作用 软骨关节 移植同种 综述
摘要:
同种异体软骨移植是治疗关节软骨缺损的有效手段,可解决自体骨软骨来源有限的问题,但如何长期保存同种异体软骨是亟待解决的问题。区别于主流的低温冷冻同种异体软骨的方法,超低温冷冻技术在添加冷冻保护剂情况下,预期可以实现同种异体软骨的长期保存。然而,此技术目前仍处于探索阶段,缺乏成熟的临床转化条件。本文介绍了同种异体软骨的超低温冷冻法,着重阐述了超低温冷冻法中的玻璃化冷冻技术,并重点综述了超低温冷冻同种异体软骨的临床应用。

参考文献/References:

[1] PEGG D E.Principles of cryopreservation[J].Methods Mol Biol,2015,1257:3-19.
[2] ABAZARI A,JOMHA N M,ELLIOTT J A,et al.Cryopreservation of articular cartilage[J].Cryobiology,2013,66(3):201-209.
[3] FAHY G M,WOWK B.Principles of cryopreservation by vitrification[J].Methods Mol Biol,2015,1257:21-82.
[4] BISSOYI A,NAYAK B,PRAMANIK K,et al.Targeting cryopreservation-induced cell death:a review[J].Biopreserv Biobank,2014,12(1):23-34.
[5] PALLANTE A L,G?TZ S,CHEN A C,et al.Treatment of articular cartilage defects in the goat with frozen versus fresh osteochondral allografts:effects on cartilage stiffness,zonal composition,and structure at six months[J].J Bone Joint Surg Am,2012,94(21):1984-1995.
[6] HAHN J,LAOUAR L,ELLIOTT J A W,et al.The effect of additive compounds on glycerol-induced damage to human chondrocytes[J].Cryobiology,2017,75:68-74.
[7] TAKRONI T A,YU H,LAOUAR L,et al.Ethylene glycol and glycerol loading and unloading in porcine meniscal tissue[J].Cryobiology,2017,74:50-60.
[8] KARLSSON J O,TONER M.Long-term storage of tissues by cryopreservation:critical issues[J].Biomaterials,1996,17(3):243-256.
[9] HUNT C J.Cryopreservation:vitrification and controlled rate cooling[J].Methods Mol Biol,2017,1590:41-77.
[10] JANG T H,PARK S C,YANG J H,et al.Cryopreservation and its clinical applications[J].Integr Med Res,2017,6(1):12-18.
[11] MULDREW K,NOVAK K,YANG H,et al.Cryobiology of articular cartilage:ice morphology and recovery of chondrocytes[J].Cryobiology,2000,40(2):102-109.
[12] SONG Y C,AN Y H,KANG Q K,et al.Vitreous preservation of articular cartilage grafts[J].J Invest Surg,2004,17(2):65-70.
[13] JOMHA N M,ELLIOTT J A,LAW G K,et al.Vitrification of intact human articular cartilage[J].Biomaterials,2012,33(26):6061-6068.
[14] BROCKBANK K G,CHEN Z Z,SONG Y C.Vitrification of porcine articular cartilage[J].Cryobiology,2010,60(2):217-221.
[15] MAEHARA M,SATO M,WATANABE M,et al.Development of a novel vitrification method for chondrocyte sheets[J].BMC Biotechnol,2013,13:58.
[16] JOMHA N M,WEISS A D,FRASER FORBES J,et al.Cryoprotectant agent toxicity in porcine articular chondrocytes[J].Cryobiology,2010,61(3):297-302.
[17] ZHANG S Z,PEGG D E.Analysis of the permeation of cryoprotectants in cartilage[J].Cryobiology,2007,54(2):146-153.
[18] JOMHA N M,LAW G K,ABAZARI A,et al.Permeation of several cryoprotectant agents into porcine articular carti-lage[J].Cryobiology,2009,58(1):110-114.
[19] FAHMY M D,ALMANSOORI K A,LAOUAR L,et al.Dose-injury relationships for cryoprotective agent injury to human chondrocytes[J].Cryobiology,2014,68(1):50-56.
[20] JUDAS F,ROSA S,TEIXEIRA L,et al.Chondrocyte viability in fresh and frozen large human osteochondral allografts:effect of cryoprotective agents[J].Transplant Proc,2007,39(8):2531-2534.
[21] GARRITY J T,STOKER A M,SIMS H J,et al.Improved osteochondral allograft preservation using serum-free media at body temperature[J].Am J Sports Med,2012,40(11):2542-2548.
[22] FORRIOL F,LONGO U G,ALVAREZ E,et al.Scanty integration of osteochondral allografts cryopreserved at low temperatures with dimethyl sulfoxide[J].Knee Surg Sports Traumatol Arthrosc,2011,19(7):1184-1191.
[23] ABAZARI A,THOMPSON R B,ELLIOTT J A,et al.Transport phenomena in articular cartilage cryopreservation as predicted by the modified triphasic model and the effect of natural inhomogeneities[J].Biophys J,2012,102(6):1284-1293.
[24] SHARDT N,AL-ABBASI K K,YU H,et al.Cryoprotectant kinetic analysis of a human articular cartilage vitrification protocol[J].Cryobiology,2016,73(1):80-92.
[25] ABAZARI A,ELLIOTT J A W,MCGANN L E,et al.MR spectroscopy measurement of the diffusion of dimethyl sulfoxide in articular cartilage and comparison to theoretical predictions[J].Osteoarthritis Cartilage,2012,20(9):1004-1010.
[26] ABAZARI A,ELLIOTT J A W, LAW G K,et al.A biomechanical triphasic approach to the transport of nondilute solutions in articular cartilage[J].Biophys J,2009,97(12):3054-3064.
[27] WU K,SHARDT N,LAOUAR L,et al.Comparison of three multi-cryoprotectant loading protocols for vitrification of porcine articular cartilage[J].Cryobiology,2020,92:151-160.
[28] WU K,LAOUAR L,DONG R,et al.Evaluation of five additives to mitigate toxicity of cryoprotective agents on porcine chondrocytes[J].Cryobiology,2019,88:98-105.
[29] TORRIE A M, KESLER W W,ELKIN J,et al.Osteochondral allograft[J].Curr Rev Musculoskelet Med,2015,8(4):413-422.
[30] HUNT H E,SADR K,DEYOUNG A J,et al.The role of immunologic response in fresh osteochondral allografting of the knee[J].Am J Sports Med,2014,42(4):886-891.
[31] FAMILIARI F,CINQUE M E,CHAHLA J,et al.Clinical outcomes and failure rates of osteochondral allograft transplantation in the knee:a systematic review[J].Am J Sports Med,2018,46(14):3541-3549.
[32] PEGG D E,WANG L,VAUGHAN D,et al.Cryopreservation of articular cartilage[J].Cryobiology,2006,52(3):347-359.
[33] CARBALLO C B,NAKAGAWA Y,SEKIYA I,et al.Basic science of articular cartilage[J].Clin Sports Med,2017,36(3):413-425.
[34] XIA Z,DUAN X,MURRAY D,et al.A method of isolating viable chondrocytes with proliferative capacity from cryopreserved human articular cartilage[J].Cell Tissue Bank,2013,14(2):267-276.
[35] RENDAL-VáZQUEZ M E,MANEIRO-PAMPíN E,RODRíGUEZ-CABARCOS M,et al.Effect of cryopreservation on human articular chondrocyte viability,proliferation,and collagen expression[J].Cryobiology,2001,42(1):2-10.
[36] FAHY G M,MACFARLANE D R,ANGELL C A,et al.Vitrification as an approach to cryopreservation[J].Cryobiology,1984,21(4):407-426.
[37] PEARSALL A W,MADANAGOPAL S G,HUGHEY J T.Osteoarticular autograft and allograft transplantation of the knee:3 year follow-up[J].Orthopedics,2008,31(1):73.
[38] ABRAMS G D,HUSSEY K E,HARRIS J D,et al.Clinical results of combined meniscus and femoral osteochondral allograft transplantation:minimum 2-year follow-up[J].Arthroscopy,2014,30(8):964-970.
[39] GERAGHTY S,KUANG J Q,YOO D,et al.A novel,cryopreserved,viable osteochondral allograft designed to augment marrow stimulation for articular cartilage repair[J].J Orthop Surg Res,2010,10:66.
[40] VANGSNESS C T,HIGGS G,HOFFMAN J K,et al.Implantation of a novel cryopreserved viable osteochondral allograft for articular cartilage repair in the knee[J].J Knee Surg,2018,31(6):528-535.
[41] WOODMASS J M,MELUGIN H P,WU I T,et al.Viable osteochondral allograft for the treatment of a full-thickness cartilage defect of the patella[J].Arthrosc Tech,2017,6(5):1661-1665.(收稿日期:2020-08-20 本文编辑:时红磊)

相似文献/References:

[1]洪郭驹,韩晓蕊.同种异体软骨低温保存技术的应用现状及影响因素[J].中医正骨,2020,32(10):56.

更新日期/Last Update: 1900-01-01