[1]冯建军,邹浩,朱海燕,等.槲皮素对去卵巢大鼠血清骨钙素和股骨Ⅰ型胶原蛋白水平的影响[J].中医正骨,2016,28(07):10-13.
　FENG Jianjun,ZOU Hao,ZHU Haiyan,et al.Leverage bone grafting and pedicle screw internal fixation through posterior approach for treatment of thoracolumbar fractures[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2016,28(07):10-13.
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槲皮素对去卵巢大鼠血清骨钙素和股骨Ⅰ型胶原蛋白水平的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第28卷
期数:
2016年07期
页码:
10-13
栏目:
基础研究
出版日期:
2016-07-20

文章信息/Info

Title:
Leverage bone grafting and pedicle screw internal fixation through posterior approach for treatment of thoracolumbar fractures
作者:
冯建军; 邹浩; 朱海燕; 甘一波
湖北省襄阳市东风人民医院,湖北 襄阳 441004
Author(s):
FENG Jianjun; ZOU Hao; ZHU Haiyan; GAN Yibo
The Dongfeng People's Hospital,Xiangyang 441004,Hubei,China
关键词:
槲皮素;  骨质疏松; 绝经后;  骨钙素;  胶原Ⅰ型;  动物实验;  大鼠
Keywords:
quercetin;  osteoporosis; postmenopausal;  osteocalcin;  collagen typeⅠ;  animal experimentation;  rats
摘要:
目的:观察槲皮素对去卵巢大鼠血清骨钙素和股骨Ⅰ型胶原蛋白水平的影响。方法:将50只6月龄清洁级健康雌性SD大鼠随机分为假手术组、模型组、尼尔雌醇组、槲皮素高剂量组和槲皮素低剂量组,每组10只。假手术组大鼠开腹找到卵巢后即关闭腹腔,其余4组切除双侧卵巢。造模手术1周后,假手术组和模型组以生理盐水灌胃,每天1次,每次4 mL; 尼尔雌醇组按1.5 mg·kg-1以尼尔雌醇灌胃,每周1次; 槲皮素高剂量组和槲皮素低剂量组分别按200 mg·kg-1和100 mg·kg-1以槲皮素灌胃,每天1次。3个月后以酶联免疫吸附测定法测定各组大鼠血清骨钙素水平,以Western Blot法测定股骨干骺端Ⅰ型胶原蛋白水平。结果:至药物干预结束时,模型组、尼尔雌醇组及槲皮素高剂量组各有1只大鼠死亡,槲皮素低剂量组2只大鼠死亡,死亡原因均为肺部感染。5组大鼠血清骨钙素水平比较,差异有统计学意义[(2.562±0.671)ng·mL-1,(5.763±0.612)ng·mL-1,(2.674±1.170)ng·mL-1,(2.722±1.063)ng·mL-1,(3.574±1.914)ng·mL-1,F=98.527,P=0.000]。假手术组、尼尔雌醇组、槲皮素高剂量组、槲皮素低剂量组血清骨钙素水平均低于模型组(P=0.000,P=0.000,P=0.000,P=0.005); 槲皮素高剂量组与尼尔雌醇组比较,差异无统计学意义(P=0.361); 槲皮素低剂量组高于尼尔雌醇组(P=0.047); 槲皮素低剂量组与槲皮素高剂组比较,差异无统计学意义(P=0.106)。5组大鼠股骨干骺端Ⅰ型胶原蛋白表达水平比较,差异有统计学意义(2.718±0.362,1.492±0.228,2.624±0.296,2.497±0.274,1.713±0.327,F=10.726,P=0.000)。假手术组、尼尔雌醇组、槲皮素高剂量组股骨Ⅰ型胶原蛋白表达水平均高于模型组(P=0.000,P=0.000,P=0.000); 槲皮素低剂量组与模型组比较,差异无统计学意义(P=0.056); 槲皮素高剂量组与尼尔雌醇组比较,差异无统计学意义(P=0.217); 槲皮素低剂量组低于槲皮素高剂量组和尼尔雌醇组(P=0.000,P=0.000)。结论:槲皮素具有类雌激素样作用,可有效降低去卵巢大鼠的血清骨钙素水平、提高股骨Ⅰ型胶原蛋白水平,其作用效果与剂量有关。
Abstract:
Objective:To observe the effect of quercetin on serum osteocalcin levels and femur typeⅠcollagen protein levels in the ovariectomized rats.Methods:Fifty healthy 6-month-old clean-grade female SD rats were randomly divided into sham-operated group,model group,nylestriol group,quercetin high-dose group and quercetin low-dose group,10 cases in each group.The rats in sham-operated group were treated with sham-operation,while the rats in other 4 groups were treated with bilateral ovariectomy.One week after the modeling,the rats in sham-operated group and model group were intragastric administrated with normal saline,once a day,4 ml at a time; and the rats in nylestriol group were intragastric administrated with nilestriol(1.5 mg/kg),once a week; and the rats in quercetin high-dose group and quercetin low-dose group were intragastric administrated with quercetin(200 and 100 mg/kg,respectively),once a day.Three months later,the serum osteocalcin levels were measured by using enzyme linked immunosorbent assays and the typeⅠcollagen protein levels of femoral metaphysis were measured by using Western Blot assays.Results:Some rats died from pulmonary infection in model group(1),nilestriol(1),quercetin high-dose group(1)and quercetin low-dose group(2)at the end of drug intervention.There was statistical difference in serum osteocalcin level between the 5 groups(2.562+/-0.671,5.763+/-0.612,2.674+/-1.170,2.722+/-1.063,3.574+/-1.914 ng/ml,F=98.527,P=0.000).The serum osteocalcin levels were lower in sham-operated group,nilestriol group,quercetinhigh-dose group and quercetin low-dose group compared to model group(P=0.000,P=0.000,P=0.000,P=0.005).There was no statistical difference in the serum osteocalcin levels between quercetin high-dose group and nilestriol group(P=0.361).The serum osteocalcin level were higher in quercetin low-dose group compared to nilestriol group(P=0.047).There was no statistical difference in serum osteocalcin levels between quercetin low-dose group and quercetin high-dose group(P=0.106).There was statistical difference in typeⅠcollagen protein levels of femoral metaphysis between the 5 groups(2.718+/-0.362,1.492+/-0.228,2.624+/-0.296,2.497+/-0.274,1.713+/-0.327,F=10.726,P=0.000).The typeⅠcollagen protein levels were higher in sham-operated group,nilestriol group and quercetin high-dose group compared to model group(P=0.000,P=0.000,P=0.000).There was no statistical difference in the typeⅠcollagen protein levels between quercetin low-dose group and model group(P=0.056).There was no statistical difference in the typeⅠcollagen protein levels between quercetin high-dose group and nilestriol group(P=0.217).The typeⅠcollagen protein levels were lower in quercetin low-dose group compared to quercetin high-dose group and nilestriol group(P=0.000,P=0.000).Conclusion:Quercetin has estrogen-like effects,which can effectively reduce serum osteocalcin levels and raise femur typeⅠcollagen protein levels in ovariectomized rats,and its effect is related to the dose.

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备注/Memo

备注/Memo:
2016-02-04收稿 2016-04-05修回
基金项目:湖北省襄阳市重点科技攻关项目[襄科计(2012)21-5]
冯建军 E-mail:jianjunfen@126.com

更新日期/Last Update: 1900-01-01