[1]许心弦,温宏,吕杨训,等.信号传导及转录激活因子1诱导骨细胞凋亡在激素性股骨头坏死过程中的作用[J].中医正骨,2014,26(02):3-7.
　Xu Xinxian*,Wen Hong,Lyu Yangxun,et al.Effect of bone cell apoptosis induced by signal transducers and activators of transcription-1 in the process of steroid-induced necrosis of femoral head[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(02):3-7.
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信号传导及转录激活因子1诱导骨细胞凋亡在激素性股骨头 坏死过程中的作用()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期数:
2014年02期
页码:
3-7
栏目:
基础研究
出版日期:
2014-02-20

文章信息/Info

Title:
Effect of bone cell apoptosis induced by signal transducers and activators of transcription-1 in the process of steroid-induced necrosis of femoral head
作者:
许心弦; 温宏; 吕杨训; 胡月正; 余华晨; 潘孝云
温州医科大学附属第二医院,浙江 温州 325027
Author(s):
Xu Xinxian*; Wen Hong; Lyu Yangxun; Hu Yuezheng; Yu Huachen; Pan Xiaoyun.
*The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,Zhejiang,China
关键词:
股骨头坏死 糖皮质激素类 细胞凋亡 信号传导 转录激活因子类
Keywords:
Femur head necrosis;  Glucocorticoids;  Apoptosis;  Signal transduction;  Activating transcription factors
摘要:
目的:探讨信号传导及转录激活因子1诱导细胞凋亡在激素性股骨头坏死过程中的作用。方法:选择30例激素性股骨头坏死组织标本,FicatⅠ期和Ⅱ期患者10例(A组)、FicatⅢ期患者10例(B组)、FicatⅣ期和Ⅴ期患者10例(C组),同时选择10例接受股骨颈骨折内固定手术的正常股骨头组织标本(D组)。对所有标本在光镜下观察测定空骨陷窝率,采用TUNEL凋亡检测法测定细胞凋亡率,分别采用免疫组化法和ELISA法对磷酸化信号传导及转录激活因子1的表达进行定性和定量检测,同时分别对细胞凋亡率与空骨陷窝率和磷酸化信号传导及转录激活因子1表达量进行相关性分析。结果:4组患者股骨头空骨陷窝率比较,差异有统计学意义[(29.10±6.14)%,(54.10±7.33)%,(75.25±7.80)%,(7.20±1.32)%,F=227.614,P=0.000]; A组空骨陷窝率高于D组(P=0.000),B组高于A组(P=0.000),C组高于B组(P=0.000)。4组患者股骨头细胞凋亡率比较,差异有统计学意义[(5.70±3.27)%,(36.90±9.53)%,(70.30±13.63)%,(2.80±2.57)%,F=136.004,P=0.000]。A组和D组比较,差异无统计学意义(P=0.209); B组高于A组(P=0.000),C组高于B组(P=0.000)。4组患者股骨头磷酸化信号传导及转录激活因子1定性检测结果比较,差异有统计学意义[(3.15±0.82),(5.97±1.01),(8.56±0.94),(1.00±0.56),F=150.005,P=0.000]。A组高于D组(P=0.000),B组高于A组(P=0.000),C组高于B组(P=0.000)。4组患者股骨头磷酸化信号传导及转录激活因子1定量检测结果比较,差异有统计学意义[(1.86±0.43),(5.04±2.24),(11.14±2.49),(1.00±0.28),F=73.466,P=0.000]。A组高于D组(P=0.000),B组高于A组(P=0.000),C组高于B组(P=0.000)。空骨陷窝率和细胞凋亡率呈正相关(rs=0.882,P=0.000),细胞凋亡率和磷酸化信号传导及转录激活因子1表达量呈正相关(rs=0.860,P=0.000)。结论:骨细胞凋亡是激素性股骨头坏死的重要过程,并且信号传导及转录激活因子1可能在其中发挥了重要作用。
Abstract:
Objective:To explore the effect of bone cell apoptosis induced by signal transducers and activators of transcription-1(STAT1)in the process of steroid-induced necrosis of femoral head(SNFH).Methods:The tissue samples of femoral head were fetched from 30 patients with SNFH.Ten patients(Group A)were categorized as Ficat phaseⅠorⅡ,10 patients(Group B)as Ficat phaseⅢ,10 patients(Group C)as Ficat phase Ⅳ or Ⅴ.Meanwhile,ten normal bone tissue samples(Group D)were obtained from internal fixation surgery for femoral neck fractures.All the samples were observed under the optical microscope and the empty bone lacuna rate were measured,then the TUNEL apoptotic detection method were used to detect the apoptosis rate.After that,qualitative and quantitative detection on the expression of phosphorylated STAT1(p-STAT1)were carried out by immunohistochemical method and ELISA method respectively.Meanwhile,correlation analysis were applied to study the relationship between empty bone lacuna rate and apoptosis rate and between empty bone lacuna rate and p-STAT1 expression.Results:There was statistical difference in the empty bone lacuna rate of femur head among the 4 groups(29.10+/-6.14%,54.10+/-7.33%,75.25+/-7.80%,7.20+/-1.32%,F=227.614,P=0.000).The empty bone lacuna rate was higher in group A compared with group D(P=0.000),was higher in group B compared with group A(P=0.000),and was higher in group C compared with group B(P=0.000).There was statistical difference in the apoptosis rate of femur head between the 4 groups(5.70+/-3.27%,36.90+/-9.53%,70.30+/-13.63%,2.80+/-2.57%,F=136.004,P=0.000).There was no statistical difference in the apoptosis rate of femur head between group A and group D(P=0.209).The apoptosis rate was higher in group B compared with group A (P=0.000)and was higher in group C compared with group B(P=0.000).There was statistical difference in the results of qualitative detection of femur head p-STAT1 between the 4 groups(3.15+/-0.82,5.97+/-1.01,8.56+/-0.94,1.00+/-0.56,F=150.005,P=0.000).Group A surpassed group D(P=0.000),group B surpassed group A(P=0.000),and group C surpassed group B(P=0.000).There was statistical difference in the results of quantitative detection of femur head p-STAT1 between the 4 groups(1.86+/-0.43,5.04+/-2.24,11.14+/-2.49,1.00+/-0.28,F=73.466,P=0.000).Group A surpassed group D(P=0.000),group B surpassed group A(P=0.000),and group C surpassed group B(P=0.000).The results of correlation analysis showed that the empty bone lacuna rate and the expression quantity of p-STAT1 were positively correlated with the apoptosis rate(rs=0.882,P=0.000; rs=0.860,P=0.000).Conclusion:STAT1 may play an important role in bone cell apoptosis,which is important for the process of SNFH.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:温州市科技局资助项目(Y20120252)
通讯作者:潘孝云 E-mail:379806041@qq.com
更新日期/Last Update: 2014-02-20