[1]林木南,李西海,刘献祥,等.动态温热疏密波对软骨细胞凋亡调控基因表达的影响[J].中医正骨,2012,24(08):3-7.
　LIN Mu-nan*,LI Xi-hai,LIU Xian-xiang,et al.The effect of dynamic warm sparse-dense wave on apoptosis regulatory genes expression of chondrocyte[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(08):3-7.
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动态温热疏密波对软骨细胞凋亡调控基因表达的影响()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期数:
2012年08期
页码:
3-7
栏目:
基础研究
出版日期:
2012-08-20

文章信息/Info

Title:
The effect of dynamic warm sparse-dense wave on apoptosis regulatory genes expression of chondrocyte
作者:
林木南1; 李西海2; 刘献祥3; 林叶青1; 曾金雄1; 刘建华1; 郭健红1; 梁永刚1; 涂向东1; 刘庆红1
1.中国人民解放军南京军区福州总医院,福建 福州 350025;
2.福建中西医结合研究院, 福建 福州 350108;
3.福建中医药大学,福建 福州 350108
Author(s):
LIN Mu-nan*; LI Xi-hai; LIU Xian-xiang; LI Ye-qing; ZENG Jin-xiong; LIU Jian-hua; GUO Jian-hong; LIANG Yong-gang; TU Xiang-dong; LIU Qing-hong.
*Fuzhou General Hospital of Nanjing Military Commang of the People's Liberation Army,Fuzhou 350025,Fujian,China
关键词:
骨性关节炎 软骨细胞 凋亡 动态温热疏密波
Keywords:
Osteoarthritis;  Chondrocyte;  Apoptosis;  Dynamic warm sparse-dense wave
摘要:
目的:探讨动态温热疏密波对骨性关节炎软骨细胞凋亡调控基因表达的影响。方法:大白兔60只,随机分为2组,即:正常组(10只)和手术组(50只)。手术组采用改良Hulth法复制膝骨性关节炎模型,术后随机分为5组,模型组造模后常规饲养,对照组微波治疗仪,实验1组电(疏密波)治疗,实验2组热(热软膜)治疗,实验3组电热(治疗型护膝动态温热疏密波)治疗,分别对应治疗16周后,观察关节软骨的组织形态学,检测软骨细胞的凋亡率及p21、p53、Bcl-2 mRNA的表达。结果:16周时,骨性关节炎模型成功建立,经治疗后各组关节软骨组织形态学病理变化程度明显轻于模型组(P<0.01,P<0.05); 各组软骨细胞的凋亡率、p53 mRNA表达,模型组、对照组、实验1组、实验2组、实验3组明显高于正常组(P<0.01),对照组、实验2组、实验3组明显低于模型组、实验1组(P<0.01,P<0.05),实验3组明显低于对照组、实验2组(P<0.01,P<0.05); 软骨细胞p21、Bcl-2 mRNA表达,模型组、对照组、实验1组、实验2组、实验3组明显低于正常组(P<0.01),对照组、实验2组、实验3组明显高于模型组、实验1组(P<0.01,P<0.05),实验3组明显高于对照组、实验2组(P<0.01,P<0.05)。结论:动态温热疏密波能有效下调软骨细胞p53 mRNA表达,上调p21、Bcl-2 mRNA表达,从而抑制软骨细胞的凋亡,延缓关节软骨退变。
Abstract:
Objective:To investigate the effect of dynamic warm sparse-dense wave on apoptosis regulatory genes expression of chondrocyte.Methods:60 white rabbits were randomly divided into normal group(n=10)and operation group(n=50).The operation group copied knee osteoarthritis by modified Hulth Law were randomly divided into 5 groups.Model group received conventional breeding after modeled,control group received the microwave therapy,experimental group 1 received electricity(sparse-dense wave)therapy,experimental group 2 received thermal(thermal soft film)therapy,and experimental group 3 received electricity and thermal(dynamic warm sparse-dense wave)therapy.After treatment 16 weeks,the morphology of articular cartilage was observed,apoptosis rate and p21,p53,Bcl-2 mRNA expression of chondrocyte was detected.Results:After 16 weeks,knee osteoarthritis model was successfully established,articular cartilage morphology in each treatment group level of pathological changes significantly reduced than its in model group(P<0.01,P<0.05).Apoptosis ate and p53 mRNA expression of chondrocyte,model group,control group,experimental group 1,experimental group 2 and experimental group 3 was significantly higher than the normal group(P<0.01),control group,experimental group 2 and experimental group 3 was significantly lower than the model group,experimental group 1(P<0.01,P<0.05),experimental group 3 was significantly lower than control group,experimental group 2(P<0.01,P<0.05); The expression of p21、Bcl-2 mRNA, Model group,control group,experimental group 1,experimental group 2,experimental group 3 was significantly lower than normal group(P<0.01),control group,experimental group 2 and experimental group 3 was significantly higher than model group,experimental group 1(P<0.01,P<0.05),experimental group 3 was significantly higher than control group,experimental group 2(P<0.01,P<0.05).Conclusion:Dynamic warm sparse-dense wave can be effect of down-regulating the expression of p53 mRNA,up-regulating the expression of p21、Bcl-2 mRNA,inhibit chondrocyte apoptosis and delay the degeneration of cartilage.

参考文献/References:

[1] Swellam M,Mahmoud MS,Samy N,et al.Potential influence of interleukin-1 receptor antagonist gene polymorphism on knee osteoarthritis risk[J].Dis Markers,2010,28(5):299.
[2] Brown DC,Boston RC,Farrar JT.Use of an activity monitor to detect response to treatment in dogs with osteoarthritis[J].J Am Vet Med Assoc,2010,237(1):66.
[3] 林木南,刘献祥,刘建华,等.OA护膝干预膝骨性关节炎的临床效应分析[J].中国中医骨伤科杂志,2007,15(11):12-15.
[4] 林木南,刘献祥,刘建华,等.治疗型关节炎护膝对实验性骨关节炎氧自由基的影响[J].中国中医骨伤科杂志,2009,17(5):19-21.
[5] 林木南,吴明霞,刘献祥,等.治疗型关节炎护膝干预膝骨性关节炎患者滑液细胞因子的研究[J].中国骨与关节损伤杂志2010,25(6):505-508.
[6] 刘献祥,李西海,周江涛.改良Hulth造模法复制膝骨性关节炎的实验研究[J].中国中西医结合杂志,2005,25(12):1104-1108.
[7] 林木南,刘献祥,李西海,等.治疗型关节炎护膝对实验性骨关节炎组织形态学的影响[J].中国组织工程研究与临床康复,2009,13(4):611-616.
[8] Little CB,Barai A,Burkhardt D,et al.Matrix metalloproteinase 13-deficient mice are resistant to osteoarthritic cartilage erosion but not chondrocyte hypertrophy or osteophyte development[J].Arthritis Rheum,2009,60(12):3723.
[9] Chen J,Gupta T,Barasz JA,et al.Analysis of microarchitectural changes in a mouse temporomandibular joint osteoarthritis model[J].Arch Oral Biol,2009,54(12):1091.
[10] Weng LH,Wang CJ,Ko JY,et al.Control of Dkk-1 ameliorates chondrocyte apoptosis,cartilage destruction,and subchondral bone deterioration in osteoarthritic knees[J].Arthritis Rheum,2010,62(5):1393.
[11] Almonte-Becerril M,Navarro-Garcia F,Gonzalez-Robles A,et al.Cell death of chondrocytes is a combination between apoptosis and autophagy during the pathogenesis of Osteoarthritis within an experimental model[J].Apoptosis,2010,15(5):631.
[12] Owen HC,Ahmed SF,Farquharson C.Chondrocyte p21(WAF1/CIP1)expression is increased by dexamethasone but does not contribute to dexamethasone-induced growth retardation in vivo[J].Calcif Tissue Int,2009,85(4):326.
[13] Sesselmann S,Süder S,Voigt R,et al.DNA methylation is not responsible for p21WAF1/CIP1 down-regulation in osteoarthritic chondrocytes[J].Osteoarthritis Cartilage,2009,17(4):507.
[14] Hashimoto S,Nishiyama T,Hayashi S,et al.Role of p53 in human chondrocyte apoptosis in response to shear strain[J].Arthritis Rheum,2009,60(8):2340.
[15] Zaman F,Menendez-Benito V,Eriksson E,et al.Proteasome inhibition up-regulates p53 and apoptosis-inducing factor in chondrocytes causing severe growth retardation in mice[J].Cancer Res,2007,67(20):10078.

备注/Memo

备注/Memo:
基金项目:中国人民解放军南京军区医学科技创新课题(09MA100)
更新日期/Last Update: 2012-08-20