[1]戴斌,沈海滨,李道龙,等.甲基强的松龙联合益肾通痹汤 对大鼠压迫性神经根损伤的保护作用[J].中医正骨,2012,24(03):9-14.
　DAI Bin*,SHEN Hai-bin,LI Dao-long,et al.The protective effect of methylprednisolone combined with YISHEN TONGBI decoction on nerve root compressive injury in rat[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(03):9-14.
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甲基强的松龙联合益肾通痹汤 对大鼠压迫性神经根损伤的保护作用()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期数:
2012年03期
页码:
9-14
栏目:
基础研究
出版日期:
2012-03-01

文章信息/Info

Title:
The protective effect of methylprednisolone combined with YISHEN TONGBI decoction on nerve root compressive injury in rat
作者:
戴斌1; 沈海滨1; 李道龙1; 王金荣1; 周克中1; 许建安2; 陈刚2; 李志伟2
1.江苏省滨海县人民医院,江苏 滨海 224500;
2.江苏省中医院,江苏 南京 210019
Author(s):
DAI Bin*; SHEN Hai-bin; LI Dao-long; WANG Jin-rong; ZHOU Ke-zhong; XU Jian-an; CHEN Gang; LI Zhi-wei.*
The People's Hospital of Binhai county,Binhai 224500,Jiangsu,China
关键词:
神经根病 益肾通痹汤 甲泼尼龙 动物实验
Keywords:
Radiculopathy;  YISHEN TONGBI DECOCTION;  Methylprednisolone;  Animal experimentation
摘要:
目的:探讨甲基强的松龙联合益肾通痹汤对大鼠压迫性神经根损伤的保护作用。方法:将90只SD大鼠随机分为5组,即空白 组、模型组、甲基强的松龙组、中药组、甲基强的松龙+中药组,每组18只。采用王拥军等建立的腰神经根压迫造模法对模型组 、甲基强的松龙组、中药组和甲基强的松龙+中药组大鼠进行造模,空白组大鼠切开背部后立即缝合。造模成功后,甲基强的松龙 组以小剂量甲基强的松龙腹腔注射,中药组以益肾通痹汤灌胃,甲基强的松龙+中药组以小剂量甲基强的松龙腹腔注射并以益肾通 痹汤灌胃; 空白组和模型组仅以相同剂量的蒸馏水灌胃。分别于造模后第7天、第14天及第28天对各组大鼠的行为学特征和神经 根损伤区变化进行观察。结果:①行为学特征:7 d时甲基强的松龙、益肾通痹汤单独使用均能降低大鼠行为学特征评分,且二者 联合使用具有协同效应(甲基强的松龙:F=61.309,P=0.000; 益肾通痹汤:F=98.078,P=0.000; 联合应用:F=39.654,P=0.001); 14 d时甲基强的松龙、益肾通痹汤单独使用均能降低大鼠行为学特征评分,且二者联合使用具有协同效应(甲基强的松 龙:F=156.902,P=0.000; 益肾通痹汤:F=196.267,P=0.000; 联合应用:F=29.512,P=0.002); 28 d时甲基强的松龙、益肾通痹汤 单独使用均能降低大鼠行为学特征评分,且二者联合使用具有协同效应(甲基强的松龙:F=96.098,P=0.000; 益肾通痹 汤:F=78.204,P=0.000; 联合应用:F=19.090,P=0.001)。②正常神经元:7 d时各组正常神经元数量比较,差异有统计学意义(F= 42.230,P=0.001); 进一步两两比较,除模型组与中药组、甲基强的松龙组与甲基强的松龙+中药组比较差异无统计学意义外 (P=0.087; P=0.069),其余各组间比较,差异均有统计学意义。14 d时各组正常神经元数量比较,差异有统计学意义 (F=23.098,P=0.002); 进一步两两比较,除模型组与甲基强的松龙组比较差异无统计学意义外(P=0.067),其余各组间比较,差异 均有统计学意义。28 d时各组正常神经元数量比较,差异有统计学意义(F=34.543,P=0.001); 进一步两两比较,除模型组与甲基 强的松龙组比较差异无统计学意义外(P=0.098),其余各组间比较,差异均有统计学意义。③变性神经元:7 d时甲基强的松龙、益 肾通痹汤单独使用均能减少变性神经元数量,且二者联合使用具有协同效应(甲基强的松龙:F=154.521,P=0.000; 益肾通痹 汤:F=102.721,P=0.000; 联合应用:F=19.512,P=0.002); 14 d时甲基强的松龙、益肾通痹汤单独使用均能减少变性神经元数量, 且二者联合使用具有协同效应(甲基强的松龙:F=54.912,P=0.000; 益肾通痹汤:F=60.451,P=0.001; 联合应 用:F=12.720,P=0.002); 28 d时甲基强的松龙、益肾通痹汤单独使用均能减少变性神经元数量,且二者联合使用具有协同效应( 甲基强的松龙:F=67.912,P=0.001; 益肾通痹汤:F=53.109,P=0.001; 联合应用:F=9.560,P=0.002)。结论:甲基强的松龙联合益 肾通痹汤能够减轻机械压迫性神经根损伤所导致的神经功能及组织形态病理学改变,促进损伤神经元修复以及正常神经元再生, 具有改善神经功能、保护神经根的作用。
Abstract:
Objective:To explore the protective effect of methylprednisolone(MP)combined with YISHEN TONGBI decoction on nerve root compressive injury in rat.Methods:Ninety Sprague-Dawley rats were randomly divided into 5 groups as blank group,model group,MP group,traditional Chinese medicine(TCM)group and MP combined with TCM group,18 cases in each group.Rats in model group,MP group,TCM group and MP combined with TCM group were built models through the compressive molding method of lumbar nerve root formulated by Wang Yong-jun,while the others in the blank group were processed with immediately suture after incision at the back.After successful modeling,rats in MP group were administrated with intraperitoneal injection of low doses of MP,cases in TCM group were administrated with gastric perfusion of YISHEN TONGBI decoction,cases in MP combined with TCM group were administrated with intraperitoneal injection of low doses of MP combined with gastric perfusion of YISHEN TONGBI decoction,while the others in blank group and model group were administrated with gastric perfusion of same doses of distilled water only.The behavioural features and changes in nerve root injury zones of the rats were observed at the 7th,the 14th and the 28th day after modeling respectively.Results:①Behavioural features:at the 7th day after modeling,the scores of behavioural features of rats were lowered down through the separately using of MP and YISHEN TONGBI decoction,and synergistic effect was found after combined application of above methods(MP:F=61.309,P=0.000; YISHEN TONGBI decoction:F=98.078,P=0.000; combined application:F=39.654,P=0.001); and above situations were also applied to those of rats at the 14th day after modeling(F=156.902,P=0.000; YISHEN TONGBI decoction:F=196.267,P=0.000; combined application:F=29.512,P=0.002)and at the 28th day after modeling (F=96.098,P=0.000; YISHEN TONGBI decoction:F=78.204,P=0.000; combined application:F=19.090,P=0.001) respectively.②The number of normal neurons:at the 7th day after modeling,there was statistical difference in the number of normal neurons among the 5 groups(F=42.230,P=0.001); there was statistical difference between any other couple groups except between model group and TCM group(P=0.087),between MP group and MP combined with TCM group(P=0.069)according to the further paired comparison approach.At the 14th day after modeling,there was statistical difference in the number of normal neurons among the 5 groups(F=23.098,P=0.002); there was statistical difference between any other couple groups except between model group and MP group(P=0.067) according to the further paired comparison approach.At the 28th day after modeling,there was statistical difference in the number of normal neurons among the 5 groups(F=34.543,P=0.001); there was statistical difference between any other couple groups except between model group and MP group(P=0.098)according to the further paired comparison approach.③The number of degenerated neurons:at the 7th day after modeling,the number of degenerated neurons were decreased through the separately using of MP and YISHEN TONGBI decoction,and synergistic effect was found after combined application of above methods(MP:F=154.521,P=0.000; YISHEN TONGBI decoction:F=102.721,P=0.000; combined application:F=19.512,P=0.002); and above situations were also applied to those of rats at the 14th day after modeling(MP:F=54.912,P=0.000; YISHEN TONGBI decoction:F=60.451,P=0.001; combined application:F=12.720,P=0.002)and at the 28th day after modeling(MP:F=67.912,P=0.001; YISHEN TONGBI decoction:F=53.109,P=0.001; combined application:F=9.560,P=0.002)respectively.Conclusion:The application of MP combined with YISHEN TONGBI decoction has the effects of improving neural functions and protecting nerve root through relieving the neurological dysfunction and pathological changes caused by nerve root compressive injury and also promoting the reparation of the injured neurons and rebirth of the normal neurons.

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备注/Memo

备注/Memo:
2011-02-25收稿 2011-06-20修回
通迅作者:戴斌 E-mail:happydaibin@sina.com
更新日期/Last Update: 2012-03-30