[1]邹光翼,李明亮,梁伟.畲药祛邪补肾汤治疗踝关节创伤后骨关节炎作用机制的实验研究[J].中医正骨,2025,37(08):4-9.
　ZOU Guangyi,LI Mingliang,LIANG Wei.Mechanism of She drug Quxie Bushen Tang(祛邪补肾汤)in treatment of post-traumatic osteoarthritis of ankle:an experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2025,37(08):4-9.
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畲药祛邪补肾汤治疗踝关节创伤后骨关节炎作用机制的实验研究()

《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第37卷
期数:
2025年08期
页码:
4-9
栏目:
基础研究
出版日期:
2025-08-20

文章信息/Info

Title:
Mechanism of She drug Quxie Bushen Tang(祛邪补肾汤)in treatment of post-traumatic osteoarthritis of ankle:an experimental study
作者:
邹光翼; 李明亮; 梁伟
丽水市人民医院,浙江 丽水 323000
Author(s):
ZOU Guangyi; LI Mingliang; LIANG Wei
Lishui People's Hospital,Lishui 323000,Zhejiang,China
关键词:
骨关节炎;  踝损伤;  软骨; 关节;  畲药;  胶原Ⅱ型;  肿瘤坏死因子-α;  动物实验;  小鼠;  祛邪补肾汤
Keywords:
osteoarthritis;  ankle injuries;  cartilage; articular;  She drugs;  collagen typeⅡ;  tumor necrosis factor-alpha;  animal experimentation;  mice;  Quxie Bushen Tang
摘要:
目的:探讨畲药祛邪补肾汤治疗踝关节创伤后骨关节炎(post-traumatic osteoarthritis,PTOA)的作用机制。方法:将18只健康的12周龄雄性C57BL/6J小鼠随机分为假手术组、模型组和祛邪补肾汤组,每组6只。模型组与祛邪补肾汤组通过横断右后肢踝关节距腓前韧带构建踝关节PTOA模型; 假手术组仅在相应位置行皮肤切口,不切韧带。造模术后24 h开始灌胃干预,每日1次,共干预12周。祛邪补肾汤组每次灌胃祛邪补肾汤药液0.4 mL(每毫升含生药1.63 g),模型组和假手术组每次灌胃等体积的生理盐水。灌胃结束后第2天,脱颈处死各组小鼠,切取小鼠右后肢踝关节制成标本。采用Micro-CT检测,分析小鼠踝关节软骨下骨的骨体积分数和骨小梁分离度; 采用阿尔辛蓝-苏木素-橙黄G染色和甲苯胺蓝染色观察小鼠踝关节软骨退变情况,采用骨形态计量分析测量系统测量小鼠踝关节软骨面积,采用国际骨关节炎研究学会(Osteoarthritis Research Society International,OARSI)半定量评分对小鼠踝关节软骨退变程度进行评估; 采用免疫组织化学染色法检测踝关节软骨中Ⅱ型胶原蛋白(collagen typeⅡ,COL2)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达水平。结果:①踝关节软骨下骨微结构分析结果。与模型组相比,祛邪补肾汤组骨体积分数大(P=0.000),骨小梁分离度小(P=0.002); 与假手术组相比,祛邪补肾汤组骨小梁分离度大(P=0.000),但2组骨体积分数的差异无统计学意义(P=0.284)。②踝关节软骨退变情况观察结果。与模型组相比,祛邪补肾汤组踝关节软骨面积大(P=0.000),软骨OARSI评分低(P=0.000); 祛邪补肾汤组与假手术组相比,踝关节软骨面积和软骨OARSI评分的组间差异均无统计学意义(P=0.542,P=0.401)。③踝关节软骨组织中COL2和TNF-α表达水平检测结果。与模型组相比,祛邪补肾汤组小鼠踝关节软骨组织中COL2阳性表达面积大(P=0.000),TNF-α阳性表达软骨细胞占比低(P=0.001); 与假手术组相比,祛邪补肾汤组踝关节软骨组织中TNF-α阳性表达软骨细胞占比高(P=0.000),但2组COL2阳性表达面积的差异无统计学意义(P=0.216)。结论:畲药祛邪补肾汤能改善踝关节PTOA模型小鼠踝关节软骨下骨微结构,延缓踝关节软骨退变,其作用机制可能与提高软骨组织中COL2水平、降低TNF-α水平有关。
Abstract:
Objective:To investigate the mechanism of She drug Quxie Bushen Tang(祛邪补肾汤,QXBST)in treatment of post-traumatic osteoarthritis(PTOA)of ankle.Methods:Eighteen 12-week-old healthy male C57BL/6J mice were selected and randomized into sham-operated group,model group,and QXBST group,with 6 ones in each group.All mice but the ones in sham-operated group were subjected to surgeries for building PTOA models by transecting the anterior talofibular ligament(ATFL)of the right hindlimb ankle,while the ones in sham-operated group were merely incised the skin at the corresponding site,without transecting ATFL.Twenty-four hours after the modeling surgery,the mice in QXBST group were intervened by intragastric administration with 0.4 mL QXBST decoction(crude drug dosage:1.63 g/mL),while the ones in model group and sham-operated group with an equal volume of normal saline,once a day for consecutive 12 weeks.On day 2 after the end of intragastric administration,all mice were sacrificed by cervical dislocation,and their right hindlimb ankle joints were harvested to make specimens for analysis.The ankle joint specimens were scanned by using a Micro-CT scanner for analyzing the subchondral bone microarchitecture such as bone volume/tissue volume(BV/TV)and trabecular separation(Tb.Sp),and the ankle joint cartilage degeneration was assessed using alcian blue-hematoxylin-orange G(ABH-OG)staining,and toluidine blue staining,respectively.Moreover,the ankle articular cartilage area was measured using a bone histomorphometry analysis and measurement system,and the cartilage degeneration severity was graded using the Osteoarthritis Research Society International(OARSI)semi-quantitative scoring system.Furthermore,the expression levels of collagen typeⅡ(COL2)and tumor necrosis factor-α(TNF-α)in the ankle cartilage tissues were detected by using immunohistochemical staining.Results:①Subchondral bone microarchitecture.The QXBST group exhibited greater BV/TV,and lower Tb.Sp compared to model group(P=0.000,P=0.002); while,compared to sham-operated group,the QXBST group exhibited greater BV/TV,with no significant difference observed in Tb.Sp(P=0.000,P=0.284).②Cartilage degeneration.The QXBST group showed larger cartilage area and lower OARSI score compared to model group(P=0.000,P=0.000),while the QXBST group was comparable to the sham-operated group in both cartilage area and OARSI score(P=0.542,P=0.401).③Expression levels of COL2 and TNF-α in the ankle cartilage tissues.The QXBST group demonstrated increased COL2-positive expression area and a lower proportion of TNF-α-positive chondrocytes compared to model group(P=0.000,P=0.001); while,compared to the sham-operated group,the QXBST group exhibited a higher proportion of TNF-α-positive chondrocytes,with no significant difference observed in COL2-positive expression area(P=0.000,P=0.216).Conclusion:She drug QXBST can improve the ankle subchondral bone microarchitecture,and attenuate ankle cartilage degeneration in the ankle PTOA model mice.It may work by up-regulating the level of COL2 and down-regulating the level of TNF-α in the ankle cartilage tissues.

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备注/Memo

备注/Memo:
基金项目:浙江省中医药优秀青年人才基金项目(2021ZQ091); 浙江省医药卫生科技计划项目(2021PY032)
通讯作者:梁伟 E-mail:doclw88@163.com
更新日期/Last Update: 1900-01-01