[1]查朱青,赵治伟.Ⅰ型牛胶原蛋白生物膜防止肌腱修复术后黏连的实验研究[J].中医正骨,2017,29(09):23-27.
　ZHA Zhuqing,ZHAO Zhiwei.An experimental study of bovine typeⅠcollagen protein biofilms for prevention of tendon adhesions after tendon repair surgery[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(09):23-27.
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Ⅰ型牛胶原蛋白生物膜防止肌腱修复术后黏连的实验研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第29卷
期数:
2017年09期
页码:
23-27
栏目:
基础研究
出版日期:
2017-09-20

文章信息/Info

Title:
An experimental study of bovine typeⅠcollagen protein biofilms for prevention of tendon adhesions after tendon repair surgery
作者:
查朱青; 赵治伟
河南省洛阳正骨医院/河南省骨科医院,河南 郑州 450016
Author(s):
ZHA Zhuqing; ZHAO Zhiwei
Luoyang Orthopedic-Traumatological Hospital,Zhengzhou 450016,Henan,China
关键词:
组织黏连;  肌腱;  生物膜;  胶原Ⅰ型;  动物实验
Keywords:
Key words tissue adhesions;  tendon;  biofilms;  collagen typeⅠ;  animal experimentation
摘要:
目的:观察Ⅰ型牛胶原蛋白生物膜防止肌腱修复术后黏连的效果,并探讨其可能的作用机理。方法:将60只3月龄CV级三黄鸡随机分为假手术组、模型组和生物膜组,每组20只。手术暴露所有三黄鸡右足第3趾趾深屈肌肌腱,暴露肌腱后假手术组直接关闭切口,模型组和生物膜组采用第3趾趾深屈肌肌腱断裂1/2再吻合建立肌腱断裂修复模型。肌腱修复后模型组直接关闭切口,生物膜组在修复的肌腱外侧包绕Ⅰ型牛胶原蛋白生物膜后关闭切口。观察实验期间各组动物健康状况、切口愈合情况及是否发生肌腱断裂。造模后第2、4、8周分别从各组选取三黄鸡,观察肌腱组织病理变化,测定肌腱滑动距离和最大抗断裂载荷。结果:实验期间各组均无动物死亡,切口均愈合良好,未发生肌腱断裂。肌腱组织病理学观察结果显示,与模型组相比,生物膜组肌腱断端成纤维细胞及胶原纤维数量明显增加,肌腱吻合端外周成纤维细胞聚集和炎性细胞浸润明显减少。造模后第2周时,3组三黄鸡第3趾趾深屈肌肌腱在1 N时的滑动距离比较,差异有统计学意义[(0.92±0.02)cm,(0.61±0.03)cm,(0.86±0.01)cm,F=196.200,P=0.000]。模型组和生物膜组的肌腱滑动距离均小于假手术组(P=0.000; P=0.017),生物膜组的肌腱滑动距离大于模型组(P=0.001)。造模后第4周时,3组三黄鸡第3趾趾深屈肌肌腱在1 N时的滑动距离比较,差异有统计学意义[(0.91±0.01)cm,(0.59±0.01)cm,(0.87±0.05)cm,F=113.977,P=0.000]。模型组的肌腱滑动距离小于假手术组和生物膜组(P=0.000; P=0.000); 生物膜组与假手术组的肌腱滑动距离比较,差异无统计学意义(P=3.651)。造模后第8周时,3组三黄鸡第3趾趾深屈肌肌腱在1 N时的滑动距离比较,差异有统计学意义[(0.91±0.05)cm,(0.58±0.01)cm,(0.90±0.04)cm,F=114.848,P=0.000]。模型组的肌腱滑动距离小于假手术组和生物膜组(P=0.000; P=0.000); 生物膜组与假手术组的肌腱滑动距离比较,差异无统计学意义(P=4.732)。造模后第2周时,3组三黄鸡第3趾趾深屈肌肌腱最大抗断裂载荷比较,差异有统计学意义[(0.99±0.01)N,(0.33±0.01)N,(0.38±0.02)N,F=2 052.221,P=0.000]。模型组的最大抗断裂载荷小于假手术组和生物膜组(P=0.000; P=0.031); 假手术组的最大抗断裂载荷大于模型组(P=0.000)。造模后第4周时,3组三黄鸡第3趾趾深屈肌肌腱最大抗断裂载荷比较,差异有统计学意义[(0.99±0.01)N,(0.78±0.01)N,(0.81±0.02)N,F=300.318,P=0.000]。模型组的最大抗断裂载荷小于假手术组和生物膜组(P=0.000; P=0.017); 假手术组的最大抗断裂载荷大于生物膜组(P=0.000)。造模后第8周时,3组三黄鸡第3趾趾深屈肌肌腱最大抗断裂载荷比较,差异有统计学意义[(0.99±0.01)N,(0.96±0.02)N,(0.97±0.01)N,F=6.065,P=0.021]。模型组的最大抗断裂载荷小于假手术组(P=0.035); 生物膜组的最大抗断裂载荷与假手术组、模型组比较,差异均无统计学意义(P=3.513; P=7.582)。结论:Ⅰ型牛胶原蛋白生物膜可以通过促进内源性愈合、减少外源性愈合防止肌腱修复术后黏连。
Abstract:
ABSTRACT Objective:To observe the effect of bovine typeⅠcollagen protein biofilms in prevention of tendon adhesions after tendon repair surgery and to explore its possible mechanism of action.Methods:Sixty 3-month-old CV-grade three-yellow chickens were randomly divided into sham-operated group,model group and biofilm group,20 cases in each group.The surgery was performed on the 3rd toe of right foot of all chickens to expose the digitorum profundus flexor tendon(DPFT).After exposure of the tendon,the incisions were closed directly in sham-operated group and the tendon rupture-and-repair models were built in model group and biofilm group through rupture and reanastomosis of half of the DPFT of the 3rd toe.The incisions were closed directly after tendon repairing in model group,while the incisions were closed after wrapping the bovine typeⅠcollagen protein biofilms around the repaired tendons in biofilm group.The health status,incision healing and tendon rupture were observed and compared between the 3 groups during the experiment.Some three-yellow chickens were selected from each group at 2,4 and 8 weeks after modeling.The pathological changes of tendon tissues were observed and the sliding distance and maximum anti-rupture load of tendons were measured.Results:All incisions healed well and no death and tendon rupture were found in the 3 groups.The results of histopathological observation on tendon tissues showed that fibroblasts and collagenous fiber increased more obviously in broken ends of tendons while fibroblasts aggregation and inflammatory cells infiltration decreased more obviously in anastomosis ends of tendons in biofilm group compared to model group.There was statistical difference in the sliding distance of DPFT of the 3rd toe at 1 N between the 3 groups at 2 weeks after modeling(0.92+/-0.02,0.61+/-0.03,0.86+/-0.01 cm,F=196.200,P=0.000).The tendon sliding distances were less in model group and biofilm group compared to sham-operated group(P=0.000; P=0.017)and were greater in biofilm group compared to model group(P=0.001).There was statistical difference in the sliding distance of DPFT of the 3rd toe at 1 N between the 3 groups at 4 weeks after modeling(0.91+/-0.01,0.59+/-0.01,0.87+/-0.05 cm,F=113.977,P=0.000).The tendon sliding distances were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.000),and there was no statistical difference in the sliding distance between biofilm group and sham-operated group(P=3.651).There was statistical difference in the sliding distance of DPFT of the 3rd toe at 1 N between the 3 groups at 8 weeks after modeling(0.91+/-0.05,0.58+/-0.01,0.90+/-0.04 cm,F=114.848,P=0.000).The tendon sliding distances were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.000),and there was no statistical difference in the sliding distance between biofilm group and sham-operated group(P=4.732).There was statistical difference in the maximum anti-rupture loads of DPFT of the 3rd toe between the 3 groups at 2 weeks after modeling(0.99+/-0.01,0.33+/-0.01,0.38+/-0.02 N,F=2 052.221,P=0.000).The maximum anti-rupture loads were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.031),and the maximum anti-rupture loads were greater in sham-operated group compared to model group(P=0.000).There was statistical difference in the maximum anti-rupture loads of DPFT of the 3rd toe between the 3 groups at 4 weeks after modeling(0.99+/-0.01,0.78+/-0.01,0.81+/-0.02 N,F=300.318,P=0.000).The maximum anti-rupture loads were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.017),and the maximum anti-rupture loads were greater in sham-operated group compared to biofilm group(P=0.000).There was statistical difference in the maximum anti-rupture load of DPFT of the 3rd toe between the 3 groups at 8 weeks after modeling(0.99+/-0.01,0.96+/-0.02,0.97+/-0.01 N,F=6.065,P=0.021).The maximum anti-rupture loads were less in model group compared to sham-operated group(P=0.035),and there was no statistical difference in the maximum anti-rupture loads between biofilm group and sham-operated group and between biofilm group and model group(P=3.513; P=7.582).Conclusion:Bovine TypeⅠcollagen protein biofilms can prevent tendon adhesions after tendon repair surgery through promoting endogenous healing and reducing exogenous healing.

参考文献/References:

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备注/Memo

备注/Memo:
通讯作者:查朱青 E-mail:zzqing0830@126.com
更新日期/Last Update: 2018-02-23