[1]罗漫丽,黄满玉,李东升,等.多种血清肿瘤相关抗原自身抗体联合检测对骨肉瘤的诊断价值研究[J].中医正骨,2021,33(04):32-37.
 LUO Manli,HUANG Manyu,LI Dongsheng,et al.A study of the clinical value of combined detection on serum autoantibodies against tumor-associated antigens in the diagnosis of osteosarcomas[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2021,33(04):32-37.
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多种血清肿瘤相关抗原自身抗体联合检测对骨肉瘤的诊断价值研究()
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《中医正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第33卷
期数:
2021年04期
页码:
32-37
栏目:
临床研究
出版日期:
2021-04-20

文章信息/Info

Title:
A study of the clinical value of combined detection on serum autoantibodies against tumor-associated antigens in the diagnosis of osteosarcomas
作者:
罗漫丽1黄满玉2李东升2马言2张勇勇2罗亚鸽2李记天2代丽萍1
(1.郑州大学基础医学院,河南 郑州 450001; 2.河南省洛阳正骨医院/河南省骨科医院,河南 郑州 450016)
Author(s):
LUO Manli1HUANG Manyu2LI Dongsheng2MA Yan2ZHANG Yongyong2LUO Yage2LI Jitian2DAI Liping1
1.School of Basic Medical Sciences of Zhengzhou University,Zhengzhou 450001,Henan,China2.Luoyang Orthopedic-Traumatological Hospital,Zhengzhou 450016,Henan,China
关键词:
骨肉瘤 抗原肿瘤 自身抗体 诊断 免疫学试验
Keywords:
osteosarcoma antigensneoplasm autoantibodies diagnosis immunologic tests
摘要:
目的:探讨多种血清肿瘤相关抗原(tumor associated antigen,TAA)自身抗体联合检测对骨肉瘤的诊断价值。方法:收集2013年12月至2016年6月收治的51例骨肉瘤患者(骨肉瘤组)和同时期在医院体检的51例健康志愿者(健康组)的外周血血清。使用间接ELISA法检测所有受试对象血清中抗烯醇化酶1(enolase 1,ENO1)自身抗体、抗甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)自身抗体、抗热休克蛋白(heat shock protein,HSP)27自身抗体、抗HSP60自身抗体、抗核仁磷酸蛋白1(nucleophosmin 1,NPM1)自身抗体、抗人PDZ和LIM域蛋白1(PDZ and LIM domain 1,PDLIM1)自身抗体、抗重组人微管解聚蛋白1(stathmin 1,STMN1)自身抗体、抗丙糖磷酸异构酶1(triosephosphate isomerase 1,TPI1)自身抗体共8种TAA自身抗体的血清含量。按单侧估计各TAA自身抗体含量的95%参考值范围,并以此计算和评价各TAA自身抗体血清含量检测诊断骨肉瘤的价值。按诊断的灵敏度由高到低排序,从诊断骨肉瘤灵敏度最高的自身抗体开始,每次增加1种灵敏度排名次一位的自身抗体,计算和评价对应的TAA自身抗体联合检测诊断骨肉瘤的价值。结果:①2组血清TAA自身抗体含量检测及对比结果。2组受试对象血清中抗ENO1自身抗体、抗GAPDH自身抗体、抗HSP27自身抗体、抗HSP60自身抗体、抗PDLIM1自身抗体、抗STMN1自身抗体和抗TPI1自身抗体含量比较,组间差异均有统计学意义(0.269±0.101,0.202±0.113,Z=-3.758,P=0.000; 0.145±0.037,0.125±0.027,Z=-3.477,P=0.001; 0.175±0.048,0.164±0.027,Z=-2.125,P=0.034; 0.174±0.048,0.149±0.028,Z=-2.871,P=0.004; 0.305±0.079,0.288±0.068,Z=-2.249,P=0.025; 0.188±0.082,0.167±0.071,Z=-2.041,P=0.041; 0.174±0.083,0.159±0.040,Z=-2.095,P=0.036); 2组受试对象血清中抗NPM1自身抗体含量比较,差异无统计学意义(0.255±0.075,0.260±0.064,Z=-0.020,P=0.984)。②血清TAA自身抗体含量检测诊断骨肉瘤的结果。除抗NPM1自身抗体之外的7种TAA自身抗体中,抗STMN1自身抗体诊断骨肉瘤的价值最大,其灵敏度为27.45%、特异度为96.08%、Youden指数为0.235 3、Kappa值为0.24。随着联合检测的TAA自身抗体种类增加,诊断的灵敏度逐渐增大、特异度逐渐减小。当联合检测抗STMN1自身抗体、抗TPI1自身抗体、抗PDLIM1自身抗体、抗ENO1自身抗体和抗HSP27自身抗体5种血清TAA自身抗体时,诊断效果比较理想,对应的灵敏度为60.78%、特异度为80.39%、Youden指数为0.411 8、符合率为70.59%、Kappa值为0.41。结论:联合检测抗STMN1自身抗体、抗TPI1自身抗体、抗PDLIM1自身抗体、抗ENO1自身抗体和抗HSP27自身抗体5种血清TAA自身抗体,对骨肉瘤具有较高的诊断价值。
Abstract:
To explore the clinical value of combined detection on serum autoantibodies against tumor associated antigens(TAA)in the diagnosis of osteosarcomas(OSs).Methods:The peripheral serum samples were collected from 51 OSs patients(OSs group)recruited from December 2013 to June 2016 and 51 healthy volunteers(healthy group)undergoing medical examination during the same period respectively.The serum levels of autoantibodies,including anti-enolase 1(anti-ENO1),anti-glyceraldehyde-3-phosphate dehydrogenase(anti-GAPDH),anti-heat shock protein 27(anti-HSP27),anti-HSP60,anti-nucleophosmin 1(anti-NPM1),anti-PDZ and LIM domain 1(anti-PDLIM1),anti-stathmin 1(anti-STMN1)and anti-triosephosphate isomerase 1(anti-TPI1),were detected by using indirect enzyme-linked immunoadsordent assay(ELISA).The clinical value of detection on the serum level of each anti-TAA autoantibody for diagnosis of OSs was calculated and evaluated based on the 95% reference range of the serum level of each anti-TAA autoantibody estimated unilaterally.According to the sort by diagnostic sensitivity from highest to lowest,starting from the autoantibody with the highest sensitivity in the diagnosis of OSs,one autoantibody with the next highest sensitivity was added each time,and the value of combined detection on corresponding anti-TAA autoantibodies in diagnosis of OSs was calculated and evaluated.Results:There were statistical difference in the serum levels of anti-ENO1,anti-GAPDH,anti-HSP27,anti-HSP60,anti-PDLIM1,anti-STMN1 and anti-TPI1 autoantibodies between the 2 groups(0.269±0.101 vs 0.202±0.113,Z=-3.758,P=0.000; 0.145±0.037 vs 0.125±0.027,Z=-3.477,P=0.001; 0.175±0.048 vs 0.164±0.027,Z=-2.125,P=0.034; 0.174±0.048 vs 0.149±0.028,Z=-2.871,P=0.004; 0.305±0.079 vs 0.288±0.068,Z=-2.249,P=0.025; 0.188±0.082 vs 0.167±0.071,Z=-2.041,P=0.041; 0.174±0.083 vs 0.159±0.040,Z=-2.095,P=0.036).There was no statistical difference in the serum level of anti-NPM1 autoantibody between the 2 groups(0.255±0.075 vs 0.260±0.064,Z=-0.020,P=0.984).The anti-STMN1 autoantibody presented with the maximum value in diagnosis of OSs among anti-ENO1,anti-GAPDH,anti-HSP27,anti-HSP60,anti-PDLIM1,anti-STMN1 and anti-TPI1 autoantibodies,with the sensitivity,specificity,Youden index and Kappa value as 27.45%,96.08%,0.235 3 and 0.24 respectively.With the increase of the number of anti-TAA autoantibodies,the diagnostic sensitivity gradually increased and the diagnostic specificity gradually decreased.The diagnostic effects were optimal when the combined detection was performed on serum autoantibodies of STMN1,TPI1,PDLIM1,ENO1 and HSP27 with the corresponding sensitivity,specificity,Youden index,total consistent rate and Kappa value as 60.78%,80.39%,0.4118,70.59% and 0.41 respectively.Conclusion:The combined detection on serum autoantibodies of STMN1,TPI1,PDLIM1,ENO1 and HSP27 enjoys high clinical value in diagnosis of OSs.

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备注/Memo

备注/Memo:
基金项目:河南省中医药科学研究专项课题(2018ZYZD01); 2019年洛阳市科技计划医疗卫生项目(1930005A)
通讯作者:代丽萍 E-mail:lpdai@zzu.edu.cn
更新日期/Last Update: 1900-01-01